Skip to main content
SpringerLink
Log in

Accumulation and within-seed distribution of iron in common bean and soybean

  • Published:
Plant and Soil Aims and scope Submit manuscript

Abstract

The influence of times of applying FeEDDHA on seed yield and Fe accumulation by four common bean (Phaseolus vulgaris L.) and two soybean (Glycine max L.) genotypes grown on a calcareous soil was studied under greenhouse conditions. The soybean genotypes, unlike the common bean genotypes, developed Fe-deficiency chlorosis and responded to application of the chelate. A preplant application of FeEDDHA was more efficacious than a flowering application in increasing seed yield of soybean. In contrast, the flowering application was much more effective than the preplant application for increasing seed Fe concentration [Fe] of both species. Percentage of seed Fe located in the seed coat of the common bean genotypes ranged from approximately 5 to 40% and was little affected by FeEDDHA. This within-seed distribution of Fe was correlated with methanol-extractable seed-coat pigments absorbing at 500 nm, presumably anthocyanins, but not with condensed tannins (proanthocyanidins). The soybean genotypes did not accumulate anthocyanins or tannins in the seed coat. Seed of Fe-deficient soybean plants without FeEDDHA had appreciably lower [Fe] and had a lower percentage of seed Fe in the seed coat than treated plants. Within-seed distribution of Fe should be considered in plant breeding because of concerns about both human nutrition and early seedling growth.

Abbreviations: DTPA – diethylenetrinitrilopentaacetic acid; EDDHA – ethylenediamine di(o-hydroxyphenylacetic acid) acid; SPAD – single photon avalanche diode

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Anonymous 1988 Recommended chemical soil test procedures for the North Central Region. Bull. 499 (rev.), North Dakota Agric. Exp. Sta., Fargo, ND, USA.

    Google Scholar 

  • Beebe S, Gonzalez A V and Rengifo J 2000 Research on trace minerals in the common bean. Food Nutr. Bull. 21, 387–391.

    Google Scholar 

  • Beninger C W and Hosfield G L 1999 Flavonal glycosides from Montcalm dark red kidney bean: Implications for the genetics of seed coat color in Phaseolus vulgaris L. J. Agric. Food Chem. 47, 4079–4082.

    CAS  PubMed  Google Scholar 

  • Beninger C W, Hosfield G L and Bassett M J 1999 Flavonoid composition of three genotypes of dry bean (Phaseolus vulgaris) differing in seedcoat color. J. Amer. Soc. Hort. Sci. 124, 514–518.

    CAS  Google Scholar 

  • Brick M A and Shanahan J F 1996 Classification and development. In Dry Bean Production and Pest Management. <nt>Eds.</nt> H F Schwartz, M A Brick, D S Nuland and G D Franc. pp. 3–11. Regional Bull. 562 A, Colorado State University, Fort Collins, CO, USA.

    Google Scholar 

  • Cakmak I 2002 Plant nutrition research: Priorities to meet human needs for food in sustainable ways. Plant Soil 247, 3–24.

    Article  CAS  Google Scholar 

  • Deshpande S S and Cheryan M 1985 Evaluation of vanillin assay for tannin analysis of dry beans. J. Food Sci. 50, 905–910.

    CAS  Google Scholar 

  • Deshpande S S and Cheryan M 1987 Determination of phenolic compounds of dry beans using vanillin, redox and precipitation assays. J. Food Sci. 57, 332–334.

    Google Scholar 

  • Ellsworth J W, Jolley V D, Nuland D S and Blaylock A D 1997 Screening for resistance to iron deficiency chlorosis in dry bean using iron reduction capacity. J. Plant Nutr. 20, 1489–1502.

    CAS  Google Scholar 

  • Frossard E, Bucher M, Mächler F, Mozafar A and Hurrell R 2000 Potential for increasing the content and bioavailability of Fe, Zn and Ca in plants for human nutrition. J. Sci. Food Agric. 80, 861–879.

    Article  CAS  Google Scholar 

  • Gomez M I, Obilana A B, Martin D F, Madzvamuse M and Monyo E S 1997 Manual of laboratory procedures for quality evaluation of sorghum and pearl millet. Tech. Manual 2, International Crops Research Institute for the Semi-Arid Tropics, Patancheru, Andra Pradesh, India.

    Google Scholar 

  • Goos R J and Johnson B E 2000 A comparison of three methods of reducing iron-deficiency in soybean. Agron. J. 92, 1135–1139.

    CAS  Google Scholar 

  • Grusak M A 2002 Enhancing mineral content in plant food products. J. Am. Coll. Nutr. 21, 178S–183S.

    Google Scholar 

  • Guzmán-Maldanado H, Castellanos J and González de Mejía E 1996 Relationship between theoretical and experimentally detected tannin content of common beans (Phaseolus vulgaris L.). Food Chem. 55, 333–335.

    Google Scholar 

  • Hale K L, Tufan H A, Pickering I J, George G N, Terry N, Pilon M and Pilon-Smits E A H 2002 Anthocyanins facilitate tungsten accumulation in Brassica. Physiol. Plant. 116, 351–358.

    Article  CAS  Google Scholar 

  • Hocking P J and Pate J S 1977 Mobilization of minerals to developing seeds of legumes. Ann. Bot. 41, 1259–1278.

    CAS  Google Scholar 

  • Johnson C D, Berry M F and Weaver C M 1985 Soybean hulls as an iron source for bread enrichment. J. Food Sci. 50, 1275–1305.

    CAS  Google Scholar 

  • Jolley V D, Cook K A, Hansen N C and Stevens W B 1996 Plant physiological responses for genotypic evaluation of iron efficiency in Strategy 1 and Strategy II plants-A review. J. Plant Nutr. 19, 1241–1255.

    CAS  Google Scholar 

  • Laszlo J A 1990 Mineral content of soybean seed coats and embryos during development. J. Plant Nutr. 13, 231–248.

    CAS  Google Scholar 

  • Lombardi-Boccia G, De Santis N, Di Lullo G and Carovale E 1995 Impact of processing on Fe dialysability from bean (Phaseolus vulgaris L.). Food Chem. 53, 191–195.

    Article  CAS  Google Scholar 

  • Marentes E and Grusak M A 1998 Iron transport and storage within the seed coat and embryo of developing seeds of pea (Pisum sativum L.). Seed Sci. Res. 8, 367–375.

    CAS  Google Scholar 

  • Marschner H 1995 Mineral Nutrition of Higher Plants, 2nd edn. Academic Press Inc., San Diego, CA, USA. 889 pp.

    Google Scholar 

  • Moraghan J T and Grafton K 1999 Seed-zinc concentration and the zinc-efficiency trait in navy bean. Soil Sci. Soc. Am. J. 63, 918–922.

    CAS  Google Scholar 

  • Moraghan J T and Grafton K 2002 Distribution of selected elements between the seed coat and embryo of two black bean cultivars. J. Plant Nutr. 25, 169–176.

    Article  CAS  Google Scholar 

  • Moraghan J T, Padilla J, Etchevers J D, Grafton K and Acosta-Gallegos J A 2002 Iron accumulation in seed of common bean. Plant Soil 246, 175–183.

    Article  CAS  Google Scholar 

  • Morel I, Cillard P and Cillard J 1998 Flavonoid-metal interactions in biological systems. In Flavonoids in Health and Disease. <nt>Eds.</nt> C A Rice-Evans and L Packer. pp. 163–177. Marcel Dekker Inc., New York, USA.

    Google Scholar 

  • Price M L, Van Scoyoc S and Butler L G 1978 A critical evaluation of the vanillin reaction as an assay for tannin in sorghum grain. J. Agric. Food Chem. 26, 1214–1218.

    Article  CAS  Google Scholar 

  • Schmidt W 1999 Mechanisms and regulation of reduction-based iron uptake in plants. New Phytol. 141, 1–26.

    Article  CAS  Google Scholar 

  • Schoonhoeven A van and Pastor Corrales M A 1987 Standard System for the Evaluation of Bean Germplasm. Centro Internactional de Agricultura Tropical (CIAT), Cali, Colombia. 53 pp.

    Google Scholar 

  • Schwartz S J 1998 Pigment analysis. In Food Analysis, 2nd ed. <nt>Ed.</nt> S S Nielsen, pp. 293–304. Aspen Publishers, Inc., Gaitersburg, MD, USA.

    Google Scholar 

  • Shen J, Zhang F, Chen Q, Rengel Z, Tang C and Song C 2002 Genotypic difference in seed iron content and early responses to iron deficiency in wheat. J. Plant Nutr. 25, 1631–1643.

    CAS  Google Scholar 

  • Shirley B W 1998 Flavonoids in seeds and grains: Physiological function, agronomic importance and the genetics of biosynthesis. Seed Sci. Res. 8, 415–422.

    CAS  Google Scholar 

  • Slabbert N 1992 Complexation of condensed tannins with metal ions. In Plant Polyphenols. <nt>Eds.</nt> R W Hemingway and P E Laks. pp. 421–436. Plenum Press, New York, USA.

    Google Scholar 

  • Subcommittee on Feed Composition 1982 United States-Canadian Tables of Feed Composition, 3rd rev. National Academy Press, Washington, DC. 148 pp.

    Google Scholar 

  • Sun B, Ricardo-da-Silva J M and Spranger I 1998 Critical factors of vanillin assay for catechins and proanthocyanidins. J. Agric. Food Chem. 46, 4267–4274.

    CAS  Google Scholar 

  • Takeoka G R, Dao L T, Full G H, Wong R Y, Haden L A, Edwards R H and Berrios J D J 1997 Characterization of black bean (Phaseolus vulgaris L.) anthocyanins. J. Agric. Food Chem. 45, 3395–3400.

    CAS  Google Scholar 

  • Todd J J and Vodkin L O 1993 Pigmented soybean (Glycine max) seed coats accumulate proanthocyanidins during development. Plant Physiol. 102, 663–670.

    CAS  PubMed  Google Scholar 

  • Welch R M, House WA, Beebe S and Cheng Z 2000 Genetic selection for enhanced bioavailable levels of iron in bean (Phaseolus vulgaris L.) seeds. J. Agric. Food Chem. 48, 3576–3580.

    Article  CAS  PubMed  Google Scholar 

  • Wikoff L R and Moraghan J T 1986 Determination of plant iron, manganese and zinc by wet ashing procedures. J. Sci. Food Agric. 37, 839–844.

    CAS  Google Scholar 

  • Wolswinkel P 1992 Transport of nutrients into developing seeds: a review of physiological mechanisms. Seed Sci. Res. 2, 59–73.

    CAS  Google Scholar 

  • Zaiter H Z, Coyne D P, Clark R B, Lindgren D T, Nordquist P T, Stroup WW and Pavlish L A 1992 Leaf chlorosis and seed yield of dry beans grown on high-pH calcareous soil following foliar iron sprays. HortSci. 27, 983–985.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Soil Science, North Dakota State University, Fargo, ND, 58105, USA

    J.T. Moraghan

Authors
  1. J.T. Moraghan
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Moraghan, J. Accumulation and within-seed distribution of iron in common bean and soybean. Plant Soil 264, 287–297 (2004). https://doi.org/10.1023/B:PLSO.0000047762.32990.6b

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/B:PLSO.0000047762.32990.6b

Search

Navigation