Abstract
Greenhouse experiments have been carried out to study the genotypic variation among 35 bean (Phaseolus vulgaris L.) genotypes with regards to tolerance to zinc (Zn) deficiency (Zn efficiency). Plants were grown for 45 days in Zn deficient soil supplemented with 0 or 5 μg Zn g−1 soil) and analyzed for Zn efficiency, plant Zn concentration and content, and the distribution of Zn between old and young parts of the shoot. Zn efficiency (ZE) was defined as the ratio of dry matter production at low and high Zn supply and was calculated for the whole shoot as well as for young and old parts of the shoot. There were marked differences in ZE among the bean genotypes. Genotypes G4449 and G11360 were about 2-fold and 10-fold more Zn-efficient than G11229 and G3871 in whole shoot and young-part based ZE, respectively. Interestingly, the older portions of the shoot for most genotypes had higher dry matter production under Zn deficiency than under sufficient Zn supply, suggesting that there was a significant inhibition of new shoot growth and transport of photosynthates from source to sink organs under low-Zn conditions. Zinc concentrations of both old and young portions of the shoot did not correlate with ZE, but shoot Zn content was found to be significantly correlated with ZE. Furthermore, Zn-efficient genotypes distributed more Zn into young parts of the shoot under Zn-deficient conditions than did the inefficient lines. Variation in seed Zn content did not significantly influence the determination of ZE. We concluded that there is a substantial variation in Zn efficiency in the bean genome, and ZE based on analysis of the young shoot tissues represents a suitable screening technique for the evaluation of ZE in low-Zn soils.
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Cakmak I, Marschner H and Bangerth F 1989 Effect of zinc nutritional status on growth, protein metabolism and levels of indole-3-acetic acid and other phytohormones in bean (Phaseolus vulgaris L.). J. Exp. Bot. 40, 405–412.
Cakmak I, Yilmaz A, Ekiz H, Torun B, Erenoglu B and Braun H J 1996 Zinc Deficiency as a critical nutritional problem in wheat production in Central Anatolia. Plant Soil 180, 165–172.
Cakmak I, Ekiz H, Yilmaz A, Torun B, Koleli N, Gultekin I, Alkan A and Eker S 1997 Differential response of rye, triticale, bread and durum wheat to Zn deficiency in calcareous soils. Plant Soil 188, 1–10.
Cakmak I, Torun, B, Erenoglu B, Oztürk L, Marschner H, Kalayci M, Ekiz H and Yilmaz A 1998 Morphological and physiological differences in cereals in response to zinc deficiency. Euphytica, 100, 349–357.
Cakmak I, Kalayci M, Ekiz H, Braun H J, Kilinc Y and Yilmaz A 1999 Zn deficiency as a practical problem in plant and human nutrition in Turkey: A NATO-Science for stability project. Field Crop Res. 60, 175–188.
Cakmak I 2000 Possible roles of zinc in protecting plant cells from damage by reactive oxygen species. New Phytol. 146, 185–205.
Cakmak I 2002 Plant nutrition research: Priorities to meet human needs for food in sustainable ways. Plant Soil 247, 3–24.
Erenoglu B, Nikolic M, Römheld V and Cakmak I 2002 Uptake and transport of foliar applied zinc (65Zn) in bread and durum wheat cultivars differing in zinc effcieny. Plant Soil 241, 251–257.
Fageria, N K and Baligar V C 1999 Phosphorus-Use efficiency in wheat genotypes. J. Plant Nutr. 22, 331–340.
Genc Y, McDonald G K and Graham R D 2000 Effect of seed zinc content on early growth of barley (Hordeum vulgare L.) under low and adequate soil zinc supply. Austr. J. Agric. Res. 51, 37–45.
Gourley C J P, Allan D L and Ruselle M P 1994 Plant nutrient efficiency. A comparison of definitions and suggested improvement. Plant Soil 15, 29–37.
Graham R D and Rengel Z 1993 Genotypic variation in Zn uptake and utilization by plants. In Zinc in Soils and Plants. Ed. A D Robson. pp. 107–114. Kluwer Academic Publishers, Dordrecht, The Netherlands.
Grotz N, Fox T, Connolly E, Park W, Guerinot M L and Eide D 1998 Identification of a family of zinc transporter genes from Arabidopsis that respond to zinc deficiency. Proc. Natl. Acad. Sci. USA 95, 7220–7224.
Hacisalihoglu G, Hart J J and Kochian L V 2001 High-and Low-Affinity Zinc Transport Systems and Their Possible Role in Zinc Efficiency in Bread Wheat. Plant Physiol. 125, 456–463.
Hacisalihoglu G and Kochian L V 2003 How do some plants tolerate low levels of soil zinc? Mechanisms of zinc efficiency in crop plants. New Phytolo. 159: 341–350.
Hacisalihoglu G, Hart J J, Wang Y, Cakmak I and Kochian L V 2003a Zinc Efficiency is Correlated with Enhanced Expression and Activity of Cu/Zn Superoxide Dismutase and Carbonic Anhydrase in Wheat. Plant Physiol. 131, 595–602.
Hacisalihoglu G, Hart J J, Vallejos C E, Kochian L V 2003b The Role of Shoot-Localized Processes in the Mechanism of Zn Efficiency in Common Bean. Planta (in press).
Islam F M A, Basford K E, Jara C, Redden R J and Beebe S 2002 Seed compositional and disease resistance differences among gene pools in cultivated common bean. Genetic Res. Crop Evol. 49, 285–293.
Judy W, Melton J, Lessmann G, Ellis B and Davis J 1965 Zinc fertilization of pea, beans, corn and sugarbeet in 1964. Mich. Agric. Exp. Stn. Farm Sci. Res. Rep. 33, 1–8.
Khan H R, McDonald G K and Rengel Z 1998 Chickpea genotypes differ in their sensitivity to Zn deficiency. Plant Soil 198, 11–18.
Kochian L V 1991 Mechanism of micronutrient uptake and translocation in plants. In Micronutrients in Agriculture 2nd ed. Eds. Martwedt et al. pp. 229–296. Soil Sci. Soc. Am. Madison, WI.
Marschner H and Cakmak I 1989 High light intensity enhances chlorosis and necrosis in leaves of zinc potassium, and magnesium deficient bean plant. J. Plant Physiol. 134, 308–315.
Moraghan J T and Grafton K 1999 Seed-Zinc Concentration and Zinc-Efficiency Trait in navy bean. Soil Sci. Soc. Am. J. 63, 918–922.
Pearson J N and Rengel Z 1995 Uptake and distribution of 65Zn and 54Mn in wheat grown at sufficient and deficient levels of Zn and Mn. I. During Vegetative Growth. J. Exp. Bot. 46, 833–39.
Polson D E 1968 A physiologic-genetic study of differential response of navy beans to zinc. Diss. Abst. 29, 450B–451B.
Rengel Z and Graham R D 1995a Wheat genotypes differ in zinc efficiency when grown in the chelate-buffered nutrient solution. I. Growth. Plant Soil 176, 307–316.
Rengel Z and Graham R D 1995b Wheat genotypes differ in zinc efficiency when grown in the chelate-buffered nutrient solution. II. Nutrient uptake. Plant Soil 176, 317–324.
Rengel Z and Römheld V 2000 Differential tolerance to Fe and Zn deficiencies in wheat germplasm. Euphytica 113, 219–225.
Rengel Z 2001 Genotypic differences in micronutrient use efficiency in crops. Comm. Soil Sci. Pl. Anal. 32, 1163–1186.
Singh S P and Westermann D T 2002 A single dominant gene controlling resistance to soil zinc deficiency in common bean. Crop Sci. 42, 1071–1074.
Streeter T C, Rengel Z, Graham R D 2001 Genotypic differences in Zn efficiency of Medicago species. Euphytica 120, 281–290.
Torun B, Bozbay G, Gultekin I, Braun H J, Ekiz H and Cakmak I 2000 Differences in shoot growth and zinc concentration of 164 bread wheat genotypes in a zinc-deficient calcareous soil. J. Plant Nutrit. 23, 1251–1265.
Viets F G, Boawn L C and Crawford C L 1953 Zinc deficiency in corn in central Washington. Agronomy J. 45, 559–565.
Welch R M 1995 Micronutrient nutrition of plants. Crit. Rev. Plant Sci. 14, 49–87.
Welch R M and Graham R D 2002 Breeding crops for enhanced micronutrient content. Plant Soil 245, 205–214.
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Hacisalihoglu, G., Ozturk, L., Cakmak, I. et al. Genotypic variation in common bean in response to zinc deficiency in calcareous soil. Plant and Soil 259, 71–83 (2004). https://doi.org/10.1023/B:PLSO.0000020941.90028.2c
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DOI: https://doi.org/10.1023/B:PLSO.0000020941.90028.2c