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Identification and analysis of expressed resistance gene sequences in wheat

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Abstract

Forty-eight resistance (R) genes conferring resistance to various types of pests have been cloned from 12 plant species. Irrespective of the host or the pest type, most R genes share a strong protein sequence similarity especially for domains and motifs. The objective of this study was to identify expressed R genes of wheat, the fraction of which is expected to be very low in the genome. Using modified RNA fingerprinting and data mining approaches we identified 220 expressed R-gene candidates. Of these, 125 sequences structurally resembled known R genes. In addition to 25–87% protein sequence similarity with the known R genes, the sequence, order, and distribution of the domains and motifs were also the same. Among the remaining 95, 17 were probable R-related, 21 were a new class of nucleotide-binding kinases, 21 were probable kinases, and 36 were p-loop-containing unknown sequences. About 76% were rare including 73 novel sequences. Three new R-gene specific motifs were also identified. Physical mapping of the 164 best R-gene candidates on 339 deletion lines localized 121 mappable R-gene candidates to 26 small chromosomal regions encompassing about 16% of the genome. About 90 of the 110 phenotypically characterized wheat R genes corresponding to 18 different pests also mapped in these regions.

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Authors and Affiliations

  1. Department of Crop and Soil Sciences, Washington State University, 227 Johnson Hall, P.O. Box 646420, Pullman, WA, 99164, USA

    Muharrem Dilbirligi & Kulvinder S. Gill

  2. Department of Agronomy and Horticulture, University of Nebraska-Lincoln, NE, 68583, USA

    Muharrem Dilbirligi & Kulvinder S. Gill

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  1. Muharrem Dilbirligi
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  2. Kulvinder S. Gill
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Correspondence to Kulvinder S. Gill.

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Dilbirligi, M., Gill, K.S. Identification and analysis of expressed resistance gene sequences in wheat. Plant Mol Biol 53, 771–787 (2003). https://doi.org/10.1023/B:PLAN.0000023663.55701.5f

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