Abstract
Four grapevine cultivars, i.e. Cabernet Sauvignon (a member of the Western Europe cultivar group), Rizamat (a member of the East cultivar group), Red Double Taste (a hybridized cultivar from Vitis vinifera L. and V. labrusca L.), and 1103Paulsen (a hybridized rootstock), were treated by three severity orders of drought stress for 25 d. Then net photosynthetic rate (P N), maximal photochemical efficiency (Fv/Fm), actual photochemical efficiency (ΦPS2) of photosystem 2, total electron transport rate (JT), and electron transport flows used in carboxylation (JC) and in oxygenation (JO) reactions catalysed by ribulose-1,5-bisphosphate carboxylase/oxygenase were determined. P N was determined again after re-watering for 2 d by gas exchange measurement. Along with the increase in severity of drought stress, P N, Fv/Fm, ΦPS2, JT, and JC in all four cultivars decreased. The range of decrease differed among cultivars. JO expressed various trends from cultivar to cultivar. In Rizamat that received slight and moderate drought stress, P N evidently decreased, but JO markedly increased, thus maintaining high values of JT and ΦPS2. Prior to the moderate drought stress, the Fv/Fm was high in Rizamat, indicating that the photodamage had not happened ahead of the moderate drought stress given. Under the severe drought stress, the photorespiration rate in Rizamat decreased by 70 %, and JT, ΦPS2, and Fv/Fm also dropped to very low values, i.e. the photodamage of photosynthetic apparatus has taken place. This suggested that the photorespiration has consumed the excessive assimilatory power and the photo-protective function of photorespiration is very important for Rizamat. When Cabernet Sauvignon grew under drought stress, its JO decreased in a small range, thus maintaining higher values of JC, JT, ΦPS2, and Fv/Fm; hence no serious photodamage occurred. Despite of the fact that P N of cv. Red Double Taste decreased markedly under the slight drought stress, JO still increased under the severe drought stress. This suggests that photorespiration is important in photoprotection under drought stress. JO in cv. 1103Paulsen markedly decreased under slight stress. Accordingly, P N, Fv/Fm, ΦPS2, JT, and JC decreased to extremely low values. Thus photorespiration effectively protects the photosynthetic apparatus from photo-damage under drought, assists in maintaining a relatively high ΦPS2, and helps P N to be rapidly recovered after re-watering.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Biehler, K., Fock, H.: Evidence for the contribution of the Mehler-peroxidase reaction in dissipating excess electrons in drought-stressed wheat.–Plant Physiol. 112: 265-272, 1996.
Brestic, M., Conic, G., Fryer, M.J., Baker, N.R.: Does photorespiration protect the photosynthetic apparatus in French bean leaves from photoinhibition during drought stress?–Planta 196: 450-457, 1995.
Cornic, G., Ghashghaie, J., Genty, B., Briantais, J.-M.: Leaf photosynthesis is resistant to a mild drought stress.-Photo-synthetica 27: 295-309, 1992.
Decker, J.P.: A rapid post-illumination deceleration of respira-tion in green leaves.–Plant Physiol. 30: 82-84, 1955.
Demmig, B., Winter, K., Krüger, A., Czygan, F.-C.: Zeaxanthin and the heat dissipation of excess light energy in Nerium oleander exposed to a combination of high light and water stress.–Plant Physiol. 87: 17-24, 1988.
Farquhar, G.D., Sharkey, T.D.: Stomatal conductance and pho-tosynthesis. – Annu. Rev. Plant Physiol. 33: 317-345, 1982.
Flexas, J., Badger, M., Chow, W.S., Medrano, J., Osmond, C.B.: Analysis of the relative increase in photosynthetic O2 uptake when photosynthesis in grapevine leaves is inhibited following low night temperatures and/or water stress.–Plant Physiol. 121: 675-684, 1999.
Gerbaud, A., Andrè, M.: Effect of CO2, O2, and light on photo-synthesis and photorespiration in wheat.–Plant Physiol. 66: 1032-1036, 1980.
Hartman, F.C., Harpel, M.R.: Structure, function, regulation, and assembly of D-ribulose-1,5-bisphosphate carboxylase/ oxygenase.-Annu. Rev. Biochem. 63: 197-234, 1994.
Haupt-Herting, S., Fock, H.P.: Exchange of oxygen and its role in energy dissipation during drought stress in tomato plants.–Physiol. Plant. 110: 489-495, 2000.
Jia, H.S.: [Relationship Between Photoinhibition and Active Oxygen Species Metabolism in Apple Leaves Under Drought Stress.]–Ph.D. Dissertation. Shandong Agricultural Univer-sity, Shandong 2001. [In Chin.]
Kozaki, A., Takeka, G.: Photorespiration protects C3 plants from photooxidation.–Nature 384: 557-560, 1999.
Long, S.P., Humphries, S., Falkowski, P.G.: Photoinhibition of photosynthesis in nature.–Annu. Rev. Plant Physiol. Plant mol. Biol. 45: 633-662, 1994.
Noctor, G., Arisi, A.C.M., Jouanin, L., Foyer, C.H.: Photorespi-ratory glycine enhances glutathione accumulation in both chloroplastic and cytosolic compartments.–J. exp. Bot. 50: 1157-1167, 1999.
Nogués, S., Alegre, L.: An increase in water deficit has no impact on the photosynthetic capacity of field-grown Mediter-ranean plants.–Funct. Plant Biol. 29: 621-630, 2002.
Orea, A., Pajuelo, P., Pajuelo, E., Quidiello, C., Romero, J.M., Márquez, A.J.: Isolation of photorespiratory mutants from Lotus japonicus deficient in glutamine synthetase.–Physiol. Plant. 115: 352-361, 2002.
Osmond, C.B., Björkman, O.: Simultaneous measurements of oxygen effects on net photosynthesis and glycolate metabo-lism in C3 and C4 species of Atriplex.–Carnegie Inst. Washington Year Book 71: 141-148, 1972.
Portis, A.R., Jr., Salvucci, M.E., Ogren, W.L.: Activation of ribulosebisphosphate carboxylase/oxygenase at physiological CO2 and ribulosebisphosphate concentrations by rubisco activase.-Plant Physiol. 82: 967-971, 1986.
Takeba, G., Kozaki, A.: Photorespiration is an essential mecha-nism for the protection of C3 plants from photooxidation.-In: Satoh, K., Murata, N. (ed.): Stress Responses of Photosynthe-tic Organisms: Molecular Mechanisms and Molecular Re-gulations. Pp. 15-36. Elsevier Science, Amsterdam 1998.
Tourneux, C., Peltier, G.: Effect of water deficit on photo-synthetic oxygen exchange measured using 18 O2 and mass spectra in plant stress physiology.–Photosynth. Res. 25: 147-150, 1990.
Valentini, R., Epron, D., de Angelis, P., Matteucci, G., Dreyer, E.: In situ estimation of net CO2 assimilation, photosynthetic electron flow and photorespiration in Turkey oak (Q. cerris L.) leaves: diurnal cycles under different levels of water supply.–Plant Cell Environ. 18: 631-640, 1995.
Wingler, A., Quick, W.P., Bungard, R.A., Bailey, K.J., Lea, P.J., Leegood, R.C.: The role of photorespiration during drought stress: an analysis utilizing barley mutants with reduced activities of photorespiratory enzymes.–Plant Cell Environ. 22: 361-373, 1999.
Zelitch, I.: Increased rate of net photosynthetic carbon dioxide uptake caused by the inhibition of glycolate oxidase.–Plant Physiol. 41: 1623-1631, 1966.
Zelitch, I.: Selection and characterization of tobacco plants with novel O2-resistant photosynthesis.–Plant Physiol. 90: 1457-1464, 1989.
Zelitch, I.: Control of plant productivity by regulation of photorespiration. Regulation of photorespiration can have beneficial effects on net photosynthesis.–BioSciences 42: 510-516, 1992.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Guan, X., Zhao, S., Li, D. et al. Photoprotective Function of Photorespiration in Several Grapevine Cultivars Under Drought Stress. Photosynthetica 42, 31–36 (2004). https://doi.org/10.1023/B:PHOT.0000040566.55149.52
Issue Date:
DOI: https://doi.org/10.1023/B:PHOT.0000040566.55149.52