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Moderately Elevated Temperature Eliminates Resistance of Rice Plants with Enhanced Expression of Glutathione Reductase to Intensive Photooxidative Stress

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Photosynthetica

Abstract

We developed transgenic rice plants (Oryza sativa L. cv. Daeribbyeo) overproducing cytosolic glutathione reductase (GR) using a GR gene from Brassica campestris and studied their response to photo-oxidative stress in the presence of methyl viologen (MV, 10 and 50 μM concentrations) under room (25 °C) and moderately elevated (35 °C) temperature by analysis of chlorophyll (Chl) a fluorescence parameters (FV/FM, qN, and qP) and of Chl content. Elevated temperature enhanced and accelerated the photo-oxidative damage to photosynthetic apparatus expressed mainly by a fast decrease of qN. Higher temperature supported the protective reaction in transformed rice plants for lower MV concentration (10 μM) and eliminated the enhanced tolerance of photosystem 2 photochemistry to photooxidative stress for higher (50 μM) MV concentration. Different mechanisms and temperature dependence of oxidative and protective reactions explain the results.

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Authors and Affiliations

  1. Laboratory of Biophysics, Faculty of Science, Palacký University, tř, Svobody 26, 771 46, Olomouc, Czech Republic

    R. Kouřil, D. Lazár & J. Nauš

  2. Department of Animal Science, Kyungpook National University, Daegu, 702701, South Korea

    H. Lee & J. Jo

Authors
  1. R. Kouřil
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  2. D. Lazár
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  3. H. Lee
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  4. J. Jo
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  5. J. Nauš
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Correspondence to J. Jo.

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Kouřil, R., Lazár, D., Lee, H. et al. Moderately Elevated Temperature Eliminates Resistance of Rice Plants with Enhanced Expression of Glutathione Reductase to Intensive Photooxidative Stress. Photosynthetica 41, 571–578 (2003). https://doi.org/10.1023/B:PHOT.0000027522.35486.f6

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  • DOI: https://doi.org/10.1023/B:PHOT.0000027522.35486.f6

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