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Chemosensitivity Assessed by Collagen Gel Droplet Embedded Culture Drug Sensitivity Test, and MDR1, MRP1, and MRP2 mRNA Expression in Human Colorectal Adenocarcinomas

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Abstract

Purpose. To evaluate chemosensitivity and its correlation with expression levels of the multidrug resistant transporter (MDR1) and the multidrug resistance-associated proteins 1 and 2 (MRP1, MRP2) mRNA in human colorectal adenocarcinomas.

Methods. Colorectal adenocarcinomas were obtained as surgical samples from 25 patients. The chemosensitivity of 12 anticancer drugs was assessed by the collagen gel droplet embedded culture drug sensitivity test (CD-DST). The expression levels of MDR1, MRP1, and MRP2 mRNA in colorectal adenocarcinomas were also evaluated by real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR).

Results. The chemosensitivity was successfully evaluated for 16 of 25 patients, and the anticancer drugs were effective against the samples showing a relatively high growth rate. Gemcitabine hydrochloride was found to be more promising than those often prescribed for the treatment of colorectal adenocarcinoma. There was no correlation of the mRNA expression levels of MDR1 and MRP1 with the chemosensitivity of any anticancer drugs tested, but mitomycin C was found to be more effective for the colorectal adenocarcinoma with relatively high expression of MRP2 mRNA.

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Authors and Affiliations

  1. Division of Diabetes, Digestive, and Kidney Diseases, epartment of Clinical Molecular Medicine, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-cho, Chuo-ku, Kobe, 650-0017, Japan

    Takako Nakahara, Takao Tamura, Chiharu Nishioka & Masato Kasuga

  2. Department of Hospital Pharmacy, School of Medicine, Kobe University, Chuo-ku, Kobe, 650-0017, Japan

    Toshiyuki Sakaeda, Tsutomu Nakamura, Noboru Okamura & Katsuhiko Okumura

  3. Department of Endoscopy, School of Medicine, Kobe University, 7-5-2 Kusunoki-cho, Chuo-ku, Kobe, 650-0017, Japan

    Nobuo Aoyama

  4. Division of Urology, Department of Organs Therapeutics, Faculty of Medicine, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-cho, Chuo-ku, Kobe, 650-0017, Japan

    Toshiro Shirakawa & Akinobu Gotoh

  5. Division of Gene Diagnosis & Gene Therapy, Department of Clinical Genetics and International Center for Medical Research, School of Medicine, Kobe University, 7-5-2 Kusunoki-cho, Chuo-ku, Kobe, 650-0017, Japan

    Akinobu Gotoh & Masafumi Matsuo

  6. Division of Gastroenterological Surgery, Department of Clinical Molecular Medicine, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-cho, Chuo-ku, Kobe, 650-0017, Japan

    Takashi Kamigaki, Masakazu Ohno & Yoshikazu Kuroda

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  1. Takako Nakahara
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  2. Toshiyuki Sakaeda
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  13. Masafumi Matsuo
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Correspondence to Katsuhiko Okumura.

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Nakahara, T., Sakaeda, T., Nakamura, T. et al. Chemosensitivity Assessed by Collagen Gel Droplet Embedded Culture Drug Sensitivity Test, and MDR1, MRP1, and MRP2 mRNA Expression in Human Colorectal Adenocarcinomas. Pharm Res 21, 406–412 (2004). https://doi.org/10.1023/B:PHAM.0000019292.03875.3e

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  • DOI: https://doi.org/10.1023/B:PHAM.0000019292.03875.3e

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