Skip to main content
SpringerLink
Log in

Preparation and Characterization of Reconstructed Small Intestinal Brush Border Membranes for Surface Plasmon Resonance Analysis

  • Published:
Pharmaceutical Research Aims and scope Submit manuscript

Abstract

Purpose. To prepare the surface generated by small intestinal brush border membrane vesicles (BBMVs) for the surface plasmon resonance (SPR) analysis, which allows the real-time measurement of binding events occurring on the intestinal membrane.

Methods. BBMVs were isolated from Sprague-Dawley rats, suspended in HEPES-buffered saline, and flowed over the surface of a SPR sensor chip composed of dextran derivatives modified with lipophilic residues. The surface coverage was determined from binding of bovine serum albumin to BBMV-immobilized sensor chip. The performance of BBMVs immobilized was evaluated by their interaction with otilonium bromide and bile salts.

Results. The stable BBMV surface was achieved when BBMV suspension was flowed over the sensor chip for 8 h at a rate of 2 μl/min. The flow of otilonium bromide resulted in an increased SPR signal because of its binding to calcium channel, which is known to be distributed over the gastrointestinal tact. When bile salts were flowed over ileal and duodenal BBMV surfaces, respectively, a slightly higher SPR signal was observed in the ileal BBMV surface, indicating the specific interaction of bile salts with bile acid transporters.

Conclusions. BBMV surfaces may be useful for the estimation of binding events on the intestinal membrane by SPR analysis, especially for the drugs that are orally administrated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. S. Terrettaz, T. Stora, C. Duschl, and H. Vogel. Protein binding to supported lipid membranes: investigation of the cholera toxin-ganglioside interaction by simultaneous impedance spectroscopy and surface plasmon resonance. Langmuir. 9:1361-1369 (1993).

    Google Scholar 

  2. K. Glasmästar, C. Larsson, F. Höök, and B. Kasemo. Protein adsorption on supported phospholipid bilayers. J. Colloid Interface Sci. 246:40-47 (2002).

    Google Scholar 

  3. D. G. Myszka and R. L. Rich. Implementing surface plasmon resonance biosensors in drug discovery. Pharm. Sci. Technol. Today 3:310-317 (2000).

    Google Scholar 

  4. R. J. Green, R. A. Frazier, K. M. Shakesheff, M. C. Davies, C. J. Roberts, and S. J. B. Tendler. Surface plasmon resonance analysis of dynamic biological interactions with biomaterials. Biomaterials 21:1823-1835.

  5. E. Sckmann. Supported membranes: scientific and practical applications. Science 271:43-48 (1996).

    Google Scholar 

  6. M. A. Cooper, A. C. Try, J. Carroll, D. J. Ellar, and D. H. Williams. Surface plasmon resonance analysis at a supported lipid monolayer. Biochim. Biophy. Acta 1373:101-111 (1998).

    Google Scholar 

  7. E. Sackmann and M. Tanaka. Supported membranes on soft polymer cushions: fabrication, characterization and applications. Trends Biotechnol. 18:58-64 (2000).

    Google Scholar 

  8. P. Artursson. and J. Karlsson. Correlation between oral drug absorption in humans and apparent drug permeability coefficients in human intestinal epithelial (Caco-2) cells. Biochem. Biophs. Res. Commun. 175:880-885 (1991).

    Google Scholar 

  9. D. M. Heuman, R. S. Bajaj, and Q. Lin. Adsorption of mixtures of bile salt taurine conjugates to lecithin-cholesterol membranes: implications for bile salt toxicity and cytoprotection. J. Lipid Res. 37:562-573 (1996).

    Google Scholar 

  10. K. Balon, B. U. Riebesehl, and B. W. Müller. Drug liposome partitioning as a tool for the prediction of human passive intestinal absorption. Pharm. Res. 16:882-888 (1999).

    Google Scholar 

  11. W. Kramer. G. Wess, G. Neckermann, G. Schubert, J. Fink, F. Girbig, U. Gutjahr, S. Kowalewski, K. H. Baringhaus, and G. Bor. Intestinal absorption of peptides by coupling to bile acids. J. Biol. Chem. 269:10621-10627 (1994).

    Google Scholar 

  12. M. J. Nowicki, B. L. Shneider, J. M. Paul, and J. E. Heubi. Glucocorticoids upregulate taurocholate transport by ileal brush-border membrane. Am. J. Physiol. 273:G197-G203 (1997).

    Google Scholar 

  13. S. A. Ibrahim and K. A. Balasubramanian. Comparative study on brush border membranes prepared from rat and monky small intestine by Ca2+ and Mg2+ precipitation. Comp. Biochem. Physiol. 112B:65-69 (1995).

    Google Scholar 

  14. R. Prabhu and K. A. Balasubramanian. A novel method of preparation of small intestinal brush border membrane vesicles by polyethylene glycol precipitation. Anal. Biochem. 289:157-161 (2001).

    Google Scholar 

  15. M. Kessler, O. Acuto, C. Storelli, H. Murer, M. Müller, and G. Semenza. A modified procedure for the rapid preparation of efficiently transporting vesicles from small intestinal brush border membranes: Their use in investigating some properties of D-glucouse and choline transport systems. Biochim. Biophy. Acta 506:136-154 (1978).

    Google Scholar 

  16. K. Walter, C. Schütt, and H. U. Bergmeyer. Methods of Enzymatic Analysis, Academic Press, New York, 1974.

    Google Scholar 

  17. M. R. Webb. A continuous spectrophotometric assay for inorganic phosphate and for measuring phosphate release kinetics in biological systems. Proc. Natl. Acad. Sci. USA 89:4884-4887 (1992).

    Google Scholar 

  18. E. Erb, X. Chen, S. Allen, C. J. Roberts, S. J. B. Tendler, M. C. Davies, and S. Forséen. Characterization of the surfaces generated by liposome binding to the modified dextran matrix of a surface plasmon resonance sensor chip. Anal. Biochem. 280:29-35 (2000).

    Google Scholar 

  19. M. A. Cooper, A. Hansson, S. Löfås, and D. H. Williams. A vesicle capture sensor chip for kinetic analysis of interactions with membrane-bound receptors. Anal. Biochem. 277:196-205 (2000).

    Google Scholar 

  20. D. J. Bjorkman, C. H. Allan, S. J. Hagen, and J. S. Trier. Structural features of absorptive cell and microvillus membrane preparations from rat small intestine. Gastroenterology 91:1401-1414 (1986).

    Google Scholar 

  21. S. Okumura, T. Akao, E. Mizuki, M. Ohba, and K. Inouye. Screening of the Bacillus thuringiensis Cry1Ac δ-endotoxin on the artificial phospholipid monolayer incorporated with brush border membrane vesicles of Plutella xylostella by optical biosensor technology. J. Biochem. Biophys. Methods 47:177-188 (2001).

    Google Scholar 

  22. M. Camilleri, and M. G. Choi Irritable bowel syndrome. Aliment. Pharmacol. Ther. 11:3-15 (1997).

    Google Scholar 

  23. A. M. Accarino, F. Azpiroz, and J. Malagelada. Selective dysfunction of mechanosensitive intestinal afferents in irritable bowel syndrome. Gastroenterology 108:636-643 (1995).

    Google Scholar 

  24. S. Evangelista, A. Giachetti, B. Chapelain, G. Neliat, and C. A. Maggi. Receptor binding profile of otilonium bromide. Pharmacol. Res. 38:111-117 (1998).

    Google Scholar 

  25. P. Santicioli, V. Zagorodnyuk, A. R. Renzetti, and C. A. Maggi. Antimuscarinic, calcium channel blocker and tachykinin NK2 receptor antagonist actions of otilonium bromide in the circular muscle of guinea-pig colon. Naunyn-Schmiedeberg's Arch. Pharmacol. 359:420-427 (1999).

    Google Scholar 

  26. A. Enhsen, W. Kramer, and G. Wess. Bile acids in drug discovery. Drug Discov. Today 3:409-418 (1998).

    Google Scholar 

  27. P. W. Swaan. F. C. Zsoka, Jr., and S. Øie. Use of the intestinal and hepatic bile acid transporters for drug delivery. Adv. Drug Deliv. Rev. 20:59-82 (1996).

    Google Scholar 

  28. W. Kramer, G. Wess, G. Schubert, M. Bickel, F. Gibig, U. Gutjahr, S. Kowalewski, K. H. Baringhaus, A. Enhsen, H. Glombik, S. Müllner, G. Neckermann, S. Schulz, and E. Petzinger. Liver-specific drug targeting by coupling to bile acids. J. Biol. Chem. 267:18598-18604 (1992).

    Google Scholar 

  29. J. L. Dupas and A. F. Hofmann. Passive jejunal absorption of bile acids in vivo: structure-activity relationships and rate limiting steps. Gastroenterology 86:A1067(1984).

    Google Scholar 

  30. G. J. Russell-Jones. Carrier-mediated transport, oral drug delivery. In E. Mathiowitz (eds), Encyclopedia of Controlled Drug Delivery, John Wiley & Sons, Inc., New York, 1999, pp. 173-184.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biomedical Research Center, Korea Institute of Science and Technology, 39-1 Haweolgog-dong, Sungbook-gu, Seoul, 136-791, Korea

    Sungpil Cho, Jae Hyung Park, Hesson Chung, Ick Chan Kwon & Seo Young Jeong

  2. Life Sciences Division, Korea Institute of Science and Technology, 39-1 Haweolgog-dong, Sungbook-gu, Seoul, 136-791, Korea

    Jaehoon Yu

  3. Department of Materials Science and Engineering, Kwangju Institute of Science and Technology, 1 Oryong-dong, Puk-gu, Kwangju, 500-712, Korea

    Yong-Kyu Lee & Youngro Byun

Authors
  1. Sungpil Cho
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jae Hyung Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jaehoon Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yong-Kyu Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Youngro Byun
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Hesson Chung
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Ick Chan Kwon
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Seo Young Jeong
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ick Chan Kwon.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cho, S., Park, J.H., Yu, J. et al. Preparation and Characterization of Reconstructed Small Intestinal Brush Border Membranes for Surface Plasmon Resonance Analysis. Pharm Res 21, 55–60 (2004). https://doi.org/10.1023/B:PHAM.0000012152.86004.aa

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/B:PHAM.0000012152.86004.aa

Search

Navigation