Skip to main content
SpringerLink
Log in

Uptake of Liposomally Entrapped Adenosine-3′-5′-Cyclic Monophosphate in Mouse Brain

  • Published:
Neurochemical Research Aims and scope Submit manuscript

Abstract

[3H] Adenosine-3′,5′-cyclic monophosphate (cAMP) could be entrapped efficiently into small unilamellar vesicles when bound to cAMP-dependent protein kinase. The leakage of [3H]cAMP protein kinase complex from liposomes was reduced by more than 60% as compared to free [3H]cAMP. Hyperosmolar mannitol increased the delivery of liposomally entrapped [3H]cAMP protein kinase to the brain with maximum uptake occurring at 10 min after mannitol administration. Optimal delivery to the brain was observed when vesicles composed of total brain lipids or phosphatidylcholine:cholesterol:sulfatides (7:2:1) were used. A slower clearance of liposomally entrapped material from brain tissue was seen under hyperosmolar conditions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

references

  1. Naoi, M. and Yagi, K. 1980. Incorporation of enzyme through blood-brain barrier into the brain by means of liposomes. Biochem. Int. 1:591–596.

    Google Scholar 

  2. Yagi, K., Naoi, M., Sakai, H., Abe, H., Konishi, H., and Arichi, S. 1982. Incorporation of enzyme into the brain by means of liposomes of novel composition. J. Appl. Biochem. 4:121–125.

    Google Scholar 

  3. Umezawa, F. and Eto, Y. 1988. Liposome targeting to mouse brain: Mannose as a recognition marker. Biochem. Biophys. Res. Commun. 153:1038–1044.

    Google Scholar 

  4. Kobayashi, K., Han, M., Watarai, S, and Yasuda, T. 1996. Availability of liposomes as drug carriers to the brain. Acta Med. Okayama 50:67–72.

    Google Scholar 

  5. Kakinuma, K., Tanaka, R., Takahashi, H., Watanabe, M., Nakagawa, T., and Kuroki, M. 1996. J. Neurosurg. 84:180–184.

    Google Scholar 

  6. Fredericks, W. R. and Rapoport, S. I. 1988. Reversible osmotic opening of blood-brain barrier in mice. Stroke 19:266–268.

    Google Scholar 

  7. Hasegawa, H. Allen, J. C., Mehta, B. M., Shapiro, W. R., and Posner, J. B. 1979. Enhancement of CNS penetration of methotrexate by hyperosmolar intracarotid mannitol in carcinomatous meningitis. Neurology 29:1280–1286.

    Google Scholar 

  8. Neuwelt, E. A., Pagel, M. A., and Dix, R. D. 1991. Delivery of ultraviolet-inactivated <sup>35</sup>S-herpes virus across an osmotically modified blood-brain barrier. J. Neurosurg. 74:475–479.

    Google Scholar 

  9. Gulati, A., Agarwal, S. K., Shukla, R., Srimal, R. C., and Dhawan, B. N. 1985. The mechanism of opening of the blood-brain barrier by hypertonic saline. Neuropharmacology 24:909–913.

    Google Scholar 

  10. Norman, A. B., Bertram, K. J., Thomas, S. R., Pratt, R. G., Samaratunga, R. C., and Sanberg, P. R. 1991. Magnetic resonance imaging of rat brain following in vivo disruption of the cerebral vasculature. Brain Res. Bull. 26:593–597.

    Google Scholar 

  11. Preparation of liposomes. Pages 45–46, in New, R. R. C. (ed.), Liposomes: A Practical Approach 1990. IRL Press, Oxford University Press, New York.

  12. McClare, C. W. F. 1971. An accurate and convenient organic phosphorous assay. Anal. Biochem. 39:527.

    Google Scholar 

  13. Frokjaer, S., Hjorth, E. L., and Worts, O. 1986. Stability testing of liposomes during storage. Pages 238–239, in Gregoriadis, G. (ed.), Liposome Technology, Vol. 1, CRC Press, Boca Raton, Fla.

    Google Scholar 

  14. Folch, J., Lees, M., and Sloane-Stanley, G. H. 1957. A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem. 226:497–509.

    Google Scholar 

  15. Tyrell, D. A., Richardson, V. J., and Ryman, B. E. 1977. The effect of serum protein fractions on liposome-cell interaction in cultured cells and the perfused rat liver. Biochem. Biophys. Acta 497:469–480.

    Google Scholar 

  16. Naoi, M. and Yagi, K. 1984. Effect of glycolipids contained in liposomes on the incorporation of β-galactosidase into specific organs. Biochem. Int. 9:267–272.

    Google Scholar 

  17. Kato, K., Yoshida, J., Kageyama, N., Kojima, N., and Yagi, K. 1988. Liposome-entrapped human interferon-β: Its pharmacokinetics and antitumor activity against human brain tumor cells. J. Clin. Biochem. Nutr. 4:139–147.

    Google Scholar 

  18. Gennuso, R., Spigelman, M. K., Chinol, M., Zappulla, R. A., Nieves, J., Vallabhajosula, S., Alberto-Paciucci, P., Goldsmith, S. J., and Holland, J. F. 1993. Effect of blood-brain barrier and blood-tumor barrier modification on central nervous system liposomal uptake. Cancer Invest. 11:118–128.

    Google Scholar 

  19. Sakamoto, A. and Ido, T. 1993. Liposome targeting to rat brain: Effect of osmotic opening of the blood-brain barrier. Brain Res. 629:171–175.

    Google Scholar 

  20. Lesage, D., Cao, A., Briane, D., Lievere, N., Coudert, R., Raphael, M., Salzmann, J., and Taillandier, E. 2002. Evaluation and optimization of DNA delivery into glioblastoma 9L cells by a cholesterol-based cationic liposome. Biochim. Biophys. Acta 1564:393–302.

    Google Scholar 

  21. Geisert, E. E. Jr., Del-Mar, N. A., Owens, J. I., and Holmberg, E. G. 1995. Transfecting neurons and glia in the rat using pH-sensitive immunoliposomes. Neurosci. Lett. 184,40–43.

    Google Scholar 

  22. Iwamoto, Y., Yang, K., Clifton, G. L., and Hayes, R. L. 1995. Liposome mediated BDNF cDNA transfer in intact and injured rat brain. Neuroreport 7:609–612.

    Google Scholar 

  23. Cao, Y. J., Shibata, T., and Rainov, N. G., 2002. Liposome-mediated transfer of the bcl-2 gene resulted in neuroprotection after in-vivo transient focal cerebral ischemia in an animal model. Gene Therap. 9:415–419.

    Google Scholar 

Download references

Author information

Author notes

  1. Shail K. Sharma

    Present address: Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, One Gustave Levy Place, New York, New York, 10029

Authors and Affiliations

  1. Department of Biochemistry, All India Institute of Medical Sciences, New Delhi-, 110029, India

    Ivone Gomes & Shail K. Sharma

Authors
  1. Ivone Gomes
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shail K. Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shail K. Sharma.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gomes, I., Sharma, S.K. Uptake of Liposomally Entrapped Adenosine-3′-5′-Cyclic Monophosphate in Mouse Brain. Neurochem Res 29, 441–446 (2004). https://doi.org/10.1023/B:NERE.0000013749.65266.a2

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/B:NERE.0000013749.65266.a2

Search

Navigation