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Effects of the Combined Treatment of Naloxone and Indomethacin on Catecholamines and Behavior After Intranigral Lipopolysaccharide Injection

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Abstract

The present study examined effects of the combined administration of naloxone (NX) and indomethacin (IM) on nigrostriatal catecholamines and locomotor activity after intranigral lipopolysaccharide (LPS) injection in Sprague-Dawley rats. NX plus IM was given 3 days after LPS injection; it significantly (P < .05) reversed LPS inflammation on nigrostriatal dopamine (DA) and nigral serotonin (5-HT) and nigral homovanillic acid (HVA)/DA ratio and nigrostriatal 5-hydroxyindoleacetic acid (5-HIAA)/5-HT ratio. It also tended to ameliorate the locomotor hyperactivity. However, NX plus IM given 30 min before LPS could not satisfactorily protect against LPS's damage both biochemically and behaviorally. These results reveal that NX plus IM may protect against LPS on DA, 5-HT, and motor function after LPS injection but not before. Thus it suggests that the combined treatment of NX and IM gives a potent therapy, but not prevention, of LPS-induced inflammation and also protect nigrostriatal dopaminergic and serotoninergic systems against LPS in rats.

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Authors and Affiliations

  1. Section of Neurology, Cardinal Tien Hospital, Taipei, Taiwan, Republic of China

    Vinchi Wang & Lie-Gan Chia

  2. Department of Medical Research, Taichung Veterans General Hospital, Taichung, (407), Taiwan, Republic of China

    Fu-Chou Cheng

  3. Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, (114), Taiwan, Republic of China

    Lie-Gan Chia

  4. Neuropharmacology Section Laboratory Pharmacology and Chemistry, NIEHS/NIH, Research Triangle Park, NC, (27709)

    Vinchi Wang & Jau-Shyong Hong

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  1. Vinchi Wang
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  4. Lee-Ju Cheng
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Correspondence to Lie-Gan Chia.

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Wang, V., Chia, LG., Ni, DR. et al. Effects of the Combined Treatment of Naloxone and Indomethacin on Catecholamines and Behavior After Intranigral Lipopolysaccharide Injection. Neurochem Res 29, 341–346 (2004). https://doi.org/10.1023/B:NERE.0000013736.80749.4b

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  • DOI: https://doi.org/10.1023/B:NERE.0000013736.80749.4b

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