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Three-Dimensional Porous Network Structure Developed in Hydroxyapatite-Based Nanocomposites Containing Enzyme Pretreated Silk Fibroin

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Abstract

Chemically modified silk fibroin (SF) with an enzyme, Proteinase K, has been incorporated into hydroxyapatite (HAp)-based nanocomposite attempting to strengthen the interfacial bonding between the mineral phase and the organic matrix. Particular emphasis is laid on the microstructure and microhardness of the composite along with the crystallographic properties of HAp. The whisker-like HAp crystallites of nanometer size show the preferential self-assembly and anisotropic crystal growth along c-axis. There appears porous microstructure with 70% of open porosity and pore size distribution of 10–115 um in the composite. Attributed to the enzyme modification, the crosslinkage between HAp clusters and SF matrix is improved to form an enhanced three-dimensional network extending throughout the composites and an increase of 35% in microhardness of the composite is achieved as well.

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Authors and Affiliations

  1. Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Yokohama, 223-8522, Japan

    Li Wang, Rei Nemoto & Mamoru Senna

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  1. Li Wang
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  2. Rei Nemoto
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  3. Mamoru Senna
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Correspondence to Mamoru Senna.

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Wang, L., Nemoto, R. & Senna, M. Three-Dimensional Porous Network Structure Developed in Hydroxyapatite-Based Nanocomposites Containing Enzyme Pretreated Silk Fibroin. Journal of Nanoparticle Research 6, 91–98 (2004). https://doi.org/10.1023/B:NANO.0000023228.49670.86

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  • DOI: https://doi.org/10.1023/B:NANO.0000023228.49670.86

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