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Mycopathologia

, Volume 158, Issue 1, pp 33–37 | Cite as

Inhibitory Effect of Aspergillus fumigatus Extract on matrix metalloproteinases Expression

  • Farshid Saadat
  • Kamiar Zomorodian
  • Mohammad Pezeshki
  • Sassan Rezaie
  • Mohammad Reza Khorramizadeh
Article

Abstract

Background: Matrix metalloproteinases (MMPs) play a role in several physiologic and pathologic events. There are some evidence indicating the involvement of MMPs inthe pathophysiology of fungal infections. Methods: Here we study somatic extract of Aspergillus fumigatus. The influence of Aspergillus vs. two other fungal extracts on MMPs production by Fibrosarcoma cell line was investigated using an in vitro cytotoxicity assay, sodium dodecyl sulfate-polyacrylamide and gelatin zymography. Results: Comparative dose-dependent inhibitory effects on MMPs were seen by A. fumigatus extract and compared with some steroidal and non-steroidal drugs. Cytotoxicity analysis of our extract revealed much lower cell death than other examined agents. Conclusions: Since inhibition of MMPs activity has been employed in modality therapy in such diseases as cancer, this extract might be promising in the preparation of anti-MMP therapeutic derivatives.

matrix metalloproteinase Aspergillus fumigatus cancer cytotoxicity zymoanalysis 

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References

  1. 1.
    Nagase H, Woessner JF. Matrix metalloproteinases. J Biol Chem 1999; 274: 21491–21494.Google Scholar
  2. 2.
    Edwards, DR, Beaudry PP et al. The roles of tissue inhibitors of metalloproteinases in tissue remodelling and cell growth. Int J Obes 1996; 20: S9–S15.Google Scholar
  3. 3.
    Cunnane G, Fitzgerald O et al. Early joint erosions and serum levels of matrix metalloproteinase 1, matrix metalloproteinase 3 and tissue inhibitor of metalloproteinase 1 in rheumatoid arthritis. Arthritis Rheum 2001; 44(10): 2263–2274.Google Scholar
  4. 4.
    Katrib A, Tak PP et al. Expression of chemokines matrix metalloproteinases in early rheumatoid arthritis. Rheumatology (Oxford) 2001; 40(9): 988–994Google Scholar
  5. 5.
    Cunnane G, Fitzgerald O et al. Synovial tissue protease gene expression and joint erosions in early rheumatoid arthritis. Arthritis Rheum 2001; 44(8): 1744-1753.Google Scholar
  6. 6.
    Bluteau G, Conrozier T et al. Matrix metalloproteinase-1, -3,-13 and aggrecanase-1 and -2 are differentially expressed in experimental osteoarthritis. Biochim Biophys Acta 2001; 1526(2): 147–158.Google Scholar
  7. 7.
    Massova I, Kotra LP et al. Matrix metalloproteinases: Structures, evolution, and diversification. FASEB J 1998; 12: 1075–1095.Google Scholar
  8. 8.
    Sanchez-Lopez R, Alexander CM et al. Role of zinc-bindingand hemopexin domain-encoded sequences in the substrate specificity of collagenase and stromelysin-2 as revealed by chimeric proteins. J Biol Chem 1993; 268: 7238–7247.Google Scholar
  9. 9.
    Gross, J, Lapiere CM. Collagenolytic activity in amphibian tissues: a tissue culture assay. Proc Natl Acad Sci U. S. A. 1962; 48: 1014–1022.Google Scholar
  10. 10.
    Imbert C, Kauffmann-Lacroix C, Daniault G, Jacquemin JL, Rodier MH. Effect of matrix metalloprotease inhibitors on the 95 kDa metallopeptidase of Candida albicans. J Antimicrob Chemother. 2002 June; 49(6): 1007–1010.Google Scholar
  11. 11.
    Gopinathan U, Ramakrishna T, Willcox M, Rao CM, Balasubramanian D, Kulkarni A, Vemuganti GK, Rao GN. Enzymatic, clinical and histologic evaluation of corneal tissues in experimental fungal keratitis in rabbits. Exp Eye Res. 2001 April; 72(4): 433–442.Google Scholar
  12. 12.
    Lovejoy B, Cleasby A et al. Structure of the catalytic domain of fibroblast collagenase complexed with an inhibitor. Science 1994; 263: 375–377.Google Scholar
  13. 13.
    Zaini F, Moore MK et al. The antigenic composition and protein profiles of eumycetoma agents. Mycoses 1991; 34: 19–28.Google Scholar
  14. 14.
    Bradford, M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72: 248–254.Google Scholar
  15. 15.
    Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680–685. 37Google Scholar
  16. 16.
    Hudson L, Hay FC. Practical Immunology. Blackwell Scientific Publications, Oxford, 1991: 65–70.Google Scholar
  17. 17.
    Heussen C, Dowdle EB. Electrophoretic analysis of plasminogen activator in polyacrylamide gels containing sodium dodecyl sulfate and copolymerized substrates. Analytical Biochemistry 1980; 102: 196–202.Google Scholar
  18. 18.
    Thant AA, Serbulea M et al. c-Ras is required for the activation of the matrix metalloproteinases by concanavalin A in 3Y1 cells. FEBS Letters 1997; 406: 28–30.Google Scholar
  19. 19.
    Fidler IJ. Molecular biology of cancer: invasion and metastasis. In Cancer: Principles and Practice of Oncology. 5th ed. Philadelphia: JB Lippincott Co, 1997: 135–152.Google Scholar
  20. 20.
    Stetler-Stevenson, WG, Hewitt R et al. Matrix metallopro-teinases and tumor invasion: from correlation and causality to the clinic. Semin Cancer Biol 1996; 7: 147–154.Google Scholar
  21. 21.
    Jones L, Ghaneh P et al. The matrix metalloproteinases and their inhibitors in the treatment of pancreatic cancer. Ann New York Acad Sci 1999; 880: 288–307.Google Scholar
  22. 22.
    Drummond AH, Bekette P et al. Preclinical and clinical studies of MMP inhibitors in cancer. Ann New York Acad Sci 1999; 878: 228–235.Google Scholar
  23. 23.
    John A, Tuszynski G. The role of matrix metalloproteinase in tumor angiogenesis and tumor metastasis. Pathol Oncol Res 2001; 7(1): 14–23.Google Scholar
  24. 24.
    Nguyen M, Arkell J et al. Human endothelial gelatinases and angiogenesis. Int J Biochem Cell Biol 2001; 33(10): 960–970.Google Scholar
  25. 25.
    Rodier MH, el Moudni B, Kauffmann-Lacroix C, Daniault G, Jacquemin JL. A Candida albicans metallopeptidase degrades constitutive proteins of extracellular matrix. FEMS Microbiol Lett. 1999; 177(2): 205–210.Google Scholar
  26. 26.
    Inokoshi J, Shiomi K et al. Funalenone, a novel collagenase in-hibitor produced by Aspergillus niger. J Antibiot 1999; 52(12): 1095–1100.Google Scholar
  27. 27.
    Eberhardt W, Schulze M et al. Glucocorticoid-mediated suppression of Cytokine-induced matrix metalloproteinase-9 expression in Rat mesangial cells: involvment of nuclear factor-kappaB and Ets transcription factors. Mol Endocrinol 2002; 16(8): 1752–1766.Google Scholar
  28. 28.
    Pross C, Farooq MM et al. Dexamethasone inhibits vascular smooth muscle cell migration via modulation of matrix metalloproteinase activity. J Surg Res 2002; 102(2): 57–62.Google Scholar
  29. 29.
    Sadowski T, Steinmeyer J. Effects of non-steroidal antiinflammatory drugs and Dexamethasone on the activity and expression of matrix metalloproteinase-1, matrix metalloproteinase-3 and tissue inhibitor of metalloproteinases-1 by bovine articular chondrocytes. Osteoarthritis Cartilage 2001; 9(5): 407–415.Google Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Farshid Saadat
    • 1
  • Kamiar Zomorodian
    • 2
  • Mohammad Pezeshki
    • 1
  • Sassan Rezaie
    • 2
  • Mohammad Reza Khorramizadeh
    • 1
  1. 1.Division of Biotechnology, Department of PathobiologyTehran University of Medical SciencesTehranIran
  2. 2.Division of Molecular Biology, Department of Medical Mycology & Parasitology, School of Public Health and Institute of Public Health ResearchTehran University of Medical SciencesTehranIran

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