Abstract
The presence of Malassezia spp. yeasts was investigated in dermatological specimens of 224 dogs, 164 dermatologically diseased and 60 normal dogs. Subjects included in the study were of different breed, age, sex and habitat. Malassezia spp. positive cultures were obtained in 142 (63.4%) specimens: 67.6% from dermatologically diseased subjects and 51.6% from healthy dogs. Malassezia pachydermatis, either as a pure culture or in association with lipid-dependent species, was identified in 138 (97%) specimens. Malassezia furfur was identified in 69 (48.6%) specimens and was associated with other Malassezia species in 68 dogs, as a pure culture in one subject: at the best of our knowledge, this species was identified before as the sole species from canine dermatitis. Malassezia sympodialis was identified in 11 (7.7%) specimens, always in association with other species: it was never isolated from kennel dogs. Statistical analysis of data showed a very significant difference (P < 0.01) in the prevalence of isolation of Malassezia spp. between animals with and without dermatological signs, and in the distribution of cultural burden between diseased and healthy dogs. A statistically significant difference (P<0.05) was also detected in the group of animals between 1- and 5-years of age. No significant difference was found between male and female dogs.
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References
Bond R, Wren L, Lloyd DH. Adherence of Malassezia pachydermatis and Malassezia sympodialis to canine, feline and human corneocytes in vitro. Vet Rec 2000; 147: 454–455.
Bond R, Howell SA, Haywood PJ, Lloyd DH. Isolation of Malassezia sympodialis and Malassezia globosa from healthy pet cats. Vet Rec 1997; 141(8): 200–201.
Bond R, Lloyd DH. Skin and mucosal populations of Malassezia pachydermatis in healthy and seborrhoeic Basset Hounds. Vet Dermatol 1997; 8: 101–106.
Bond R, Anthony RM, Dodd M, Lloyd DH. Isolation of Malassezia sympodialis from feline skin. J Med Vet Mycol 1996; 34(2): 145–147.
Bond R, Anthony RM. Characterization of markedly lipiddependent Malassezia pachydermatis isolates from healthy dogs. J Appl Bacteriol 1995; 78 (59): 537–542.
Bond R, Collins N S, Lloyd DH. Use of contact plates for the quantitative culture of Malassezia pachydermatis from canine skin. J Small Anim Pract 1994; 35: 68–72.
Carlotti DN, Taillieu-Le Roy S. L'otite externe chez le chien: étiologie et clinique, revue bibliographyque et étude retrospective portant sur 752 cas. PratMéd Chir Anim Comp 1997; 32: 243–257.
Crespo MJ, Abarca ML, Cabanes FJ. Occurrence of Malassezia spp. in the external ear canals of dogs and cats with and without otitis externa. Med Mycol 2002; 40: 115–121.
Crespo MJ, Abarca ML, Cabanes FJ. Atypical lipid-dependent Malassezia species isolated from dogs with otitis externa. J Clin Microbiol 2000; 38(6): 2383–2385.
Duarte EP, Melo MM, Hahn RC, Hamdan JS. Prevalence of Malassezia spp. in the ears of asymptomatic cattle and cattle with otitis in Brazil. Med Mycol 1999; 37(3): 159–162.
Guého E, Boekhout T, Ashbee HR, Guillot J, Van Belkum A. The role of Malassezia species in the ecology of human skin and as pathogens. Med Mycol 1998; 36 Suppl 1: 220–229.
Guého E, Midgley G, Guillot J. The genus Malassezia with description of four new species. Antonie van Leeuwenhoek 1996; 69: 337–355.
Guého E, Simmons RB, Pruitt WR, Meyer SA, Ahearn DG. Association of Malassezia pachydermatis with systemic infections of humans. J Clin Microbiol 1987; 25: 1789–1790.
Guillot J, Bond R. Malassezia pachydermatis: a review. Med Mycol 1999; 37: 295–306.
Guillot J, Guého E, Mialot M, Chermette R. Importance des levures du genre Malassezia en dermatologie vétérinarie. Le Point Vétérinaire 1998; 29 (193): 691–701.
Guillot J, Guého E, Lesourd M, Midgley G, Chévrier G, Dupont B. Identification of Malassezia species. A practical approach. J Mycol Méd 1996; 6: 103–110.
Guillot J, Chermette R. Guého E. Prevalence du genre Malassezia chez les Mammiféres. J Mycol Méd 1994; 4: 72–79.
Kennis PA, Rosser, EJ Jr, Olivier NB, Walker RW. Quantity and distribution ofMalassezia organisms on the skin of clinically normal dogs. J Am Vet Med Assoc 1996; 208 (7): 1048–1051.
Mauldin ED, Scott DW, Miller WH, Smith CA. Malassezia dermatitis in the dog: a retrospective histopathological and immunopathological study of 86 cases (1990–1995) Vet Dermatol 1997; 8: 191–202.
Mayser P, Haze P, Papavassilis C, Pickel M, Gruender K. Guého E. Differentiation of Malassezia species: selectivity of Cremophor EL, castor oil and ricinoleic acid for Malassezia furfur. Br J Dermatol 1997; 137(2): 208–213.
Mayser P, Wille G, Imkampe A, Thoma W, Arnold N, Monsees T. Synthesis of fluorochromes and pigments in Malassezia furfur by use of tryptophan as the single nitrogen source. Mycoses 1998; 41: 265–271.
Morris DO. Malassezia dermatitis and otitis. Vet Clin North Am Small Anim Pract. 1999; 29(6): 1303–1310.
Raabe P, Mayser P, Weiβ R. Demonstration ofMalassezia furfur and M. sympodialis together with M. pachydermatis in veterinary specimens. Mycoses 1998; 41: 493–500.
Reberg-Bruner S, Blakemore JC. Malassezia dermatitis in dogs. Vet Med 1999; July: 613–622.
Rum A, Mancianti F, Corazza M, Nardoni S. Prevalenza di specie di Malassezia nel cane e nel gatto. Proceedings of the FIMUA National Meeting, 5th ed. Bari, 7–9 September 2000: 14.
Sharma VD, Rhoades HE. The occurrence and microbiology of otitis externa in the dog. Small An Pract 1975; Apr 16(4): 241–247.
Wagner R, Schadler S. Qualitative study of Malassezia species colonisation in young puppies. Vet Rec 2000; 147: 192–194.
Willemse T. Atopic skin disease: a review and reconsideration of diagnostic criteria. J Small An Pract 1986; 27: 771–778.
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Nardoni, S., Mancianti, F., Corazza, M. et al. Occurrence of Malassezia species in healthy and dermatologically diseased dogs. Mycopathologia 157, 383–388 (2004). https://doi.org/10.1023/B:MYCO.0000030416.36743.dd
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DOI: https://doi.org/10.1023/B:MYCO.0000030416.36743.dd