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Enzyme Production by the Mycoparasite Verticillium biguttatum against Rhizoctonia solani

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Abstract

Verticillium biguttatum, a mycoparasite of the ubiquitous soil-borne plant pathogen Rhizoctonia solani, excreted chitinase and β-1,3-glucanase into liquid medium when grown on laminarin and chitin, respectively. Neither chitinase nor β-1,3-glucanase was produced by the mycoparasite when grown on cell walls of two isolates of R. solani representing anastomosis groups (AG)-3 and AG-8. Extracellular protease was induced by growth on cell walls of the pathogen, whereas β-1,3-glucanase and chitinase were produced bound to the cell wall of V. biguttatum. This is the first report of chitinase, β-1,3-glucanase and protease production by V. biguttatum. These enzymes may play a previously unforeseen role in dissolving and penetrating the cell walls of R. solani.

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Authors and Affiliations

  1. Department of Plant Biology, Scottish Agricultural College, Ayr Campus, Auchincruive Estate, Ayr, KA6 5HW, UK

    Mark P. McQuilken & Jacqueline Gemmell

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  1. Mark P. McQuilken
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  2. Jacqueline Gemmell
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Correspondence to Mark P. McQuilken.

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McQuilken, M.P., Gemmell, J. Enzyme Production by the Mycoparasite Verticillium biguttatum against Rhizoctonia solani . Mycopathologia 157, 201–205 (2004). https://doi.org/10.1023/B:MYCO.0000020590.20040.4a

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  • DOI: https://doi.org/10.1023/B:MYCO.0000020590.20040.4a

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