Abstract
Eleven geographical isolates of the entomopathogenic fungusNomuraea rileyi (Farlow) Samson of Helicoverpa armigera (Hubner)/Spodoptera litura(Fabricius) origin were studied for efficacy against the two host insects. Laboratory bioassays at a concentrationof 2 × 108 conidiaml-1 indicated that N. rileyi isolates of S. lituraorigin were better in terms of time taken for mycosis and mortality in both the test larvae: S. litura(77–80% mortality in 7 days) and H. armigera (79–85% in 8 days). Among the isolates of S. litura origin, geographical isolates from Hyderabad and Karimnagar were superior in terms of high percentkill as well as 100% germination of conidia within 48 h. Fastest germination was observed withKarimnagar isolate followed by Hyderabad isolate. Conidial yield was highest on barley-carrotextract-yeast extract medium. However in terms of material cost, barley-yeast extract medium was the lowest.The Karimnagar isolate of S. litura origin gave the highest conidial yield on barley-yeast extract medium.Chitinolytic enzyme profiles of different isolates revealed polymorphism in all the isolates from S. lituraorigin. Overall among the parameters studied the best traits were found in the Karimnagar isolate ofS. litura origin.
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Vimala Devi, P., Prasad, Y., Anitha Chowdary, D. et al. Identification of Virulent Isolates of the Entomopathogenic Fungus Nomuraea Rileyi (F) Samson for the Management of Helicoverpa Armigera and Spodoptera Litura . Mycopathologia 156, 365–373 (2003). https://doi.org/10.1023/B:MYCO.0000003578.38483.34
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DOI: https://doi.org/10.1023/B:MYCO.0000003578.38483.34