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Caveolae: biochemical analysis

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Abstract

aveolae appear in a multitude of processes encompassing growth regulation andtrafficking. We demonstrate the abundant presence of ESA/reggie-1/flotillin-2,ATP synthase β subunit and annexin V in endothelial caveolae byimmunopurification of caveolae from vascular endothelial membrane. Fiveproteins are abundant in a caveolin-1 protein complex, analyzed by sucrosegradient velocity sedimentation following octyl-β-D-glucopyranosideextraction. Caveolin-1 α interacts with caveolin-1 β, caveolin-2, actin,the microsomal form of NADH cytochrome B5 reductase and ESA/reggie-1/flotillin-2as shown by co-immunoprecipitation. We propose the concept that ATP biosynthesisin caveolae regulates mechanosignaling and is induced by membrane depolarizationand a proton gradient. Pressure stimuli and metabolic changes may trigger generegulation in endothelial cells, involving a nuclear conformer of caveolin-1,shown here with an epitope-specific caveolin-1 antibody, and immediate responseof ion channel activity, regulated by ESA/reggie-1/flotillin-2.

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  1. Christian Chatenay-Rivauday

    Present address: Novartis Ophtalmics, Research, CH-4002, Basel, Switzerland

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  1. Division of Biochemistry, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056, Basel, Switzerland

    Christian Chatenay-Rivauday, Z. Petek Cakar, Paul Jenö, Elena S. Kuzmenko & Klaus Fiedler

  2. Department of Molecular Biology and Genetics, Istanbul Technical University, T-80626, Maslak, Turkey

    Z. Petek Cakar

  3. Pediatric Surgery, Kantonsspital Basel, CH-4031, Basel, Switzerland

    Elena S. Kuzmenko

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Chatenay-Rivauday, C., Cakar, Z.P., Jenö, P. et al. Caveolae: biochemical analysis. Mol Biol Rep 31, 67–84 (2004). https://doi.org/10.1023/B:MOLE.0000031352.51910.e9

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