Abstract
Potato Cyst Nematodes (PCN) currently represent a serious threat to potato cultivation. However, many sources of resistance are known amongst primitive and wild relatives of cultivated potato, Solanum tuberosum ssp. tuberosum. Currently, in the UK, the major threat is due to Globodera pallida, resistance to which has not yet been effectively deployed in potato cultivars. We have performed linkage and QTL analysis of a tetraploid potato population segregating for the ‘H3’ source of resistance to G. pallida that was introgressed into cultivated potato from the primitive species, Solanum tuberosum ssp. andigena. This source is highly effective against the most common UK pathotype of G. pallida (Pa2/3) and its deployment in breeding material is a major goal. We adopted an approach involving bulked segregant analysis (BSA) as well as genome wide linkage analysis using AFLP and SSR markers. BSA provided a concentration of markers linked in coupling to a QTL on linkage group IV, and improved the accuracy of the QTL localisation. By performing an analysis on residual scores after removal of the effects due to the major QTL, we detected a second QTL on linkage group XI.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bradshaw J.E., Meyer R.C., Milbourne D., McNicol J.W., Phillips M.S. and Waugh R. 1998. Identification of AFLP and SSR markers associated with quantitative resistance to Globodera pallida(Stone) in tetraploid potato (Solanum tuberosumsubsp. tuberosum) with a view to marker-assisted selection. Theor. Appl. Genet. 97: 202–210.
Bryan G.J., McLean K., Bradshaw J.E., Phillips M., Castelli L., De Jong W.S. and Waugh R. 2002. Mapping QTL for resistance to the cyst nematode Globodera pallidaderived from the wild potato species Solanum vernei. Theor. Appl. Genet. 105: 68–77.
Buntjer J.B. 1999.Cross Checker Fingerprint analysis software v2.9. Wageningen University and Research Centre, The Netherlands.
Cardle L., Ramsay L., Milbourne D., Macaulay M., Marshall D. and Waugh R. 2000. Computational and experimental characterization of physically clustered simple sequence repeats in plants. Genetics 156: 847–854.
Churchill G.A. and Doerge R.W. 1994. Empirical threshold values for quantitative trait mapping. Genetics 138: 963–971.
Ernst K., Kumar A., Kriseleit D., Kloos D.U., Phillips M.S., and Ganal M.W. 2002. The broad-spectrum potato cyst nematode resistance gene (Hero) from tomato is the only member of a large gene family of NBS- LRR genes with an unusual amino acid repeat in the LRR region.Plant J. 31: 127-136.
Hackett C.A., Bradshaw J.E. and McNicol J.W. 2001. Interval mapping of quantitative trait loci in autotetraploid species. Genetics 159: 1819–1832.
Hackett C.A. and Luo Z.W. 2003. TetrapbidMap: construction of a linkage map in autotetraploid species. Journal of Heredity 94: 358–359.
Hackett C.A., Pande B. and Bryan G.J. 2003. Linkage mapping in autotetraploid species using simulated annealing. Theor. Appl. Genet. 106: 1107–1115.
Isidore E., van Os H., Andrzejewski S., Bakker J., Barrena I., Bryan G.J., Caromel B., van Eck H., Ghareeb B., De Jong W., van Koert P., Lefebvre V., Milbourne D., Ritter E., Rouppe van der Voort J., Rousselle-Bourgeois F., van Vliet J. and Waugh R. 2003. Toward a marker-dense meiotic map of the potato genome: Lessons from linkage group I. Genetics 165: 2107–2116.
Jansen R.C. 1992. A general mixture model for mapping quantitative trait loci by using molecular markers. Theor. Appl. Genet. 85: 252–260.
Kanyuka K., Bendahmane A., Rouppe van der Voort J., van der Vossen E.A.G. and Baulcombe D.C. 1999. Mapping of intra locus duplications and introgressed DNA: aids to map-based cloning of genes from complex genomes illustrated by physical analysis of the Rx locus in tetraploid potato. Theor. Appl. Genet. 98: 679–689.
Luo Z.W., Hackett C.A., Bradshaw J.E., McNicol J.W. and Milbourne D. 2000. Predicting parental genotypes and gene segregation for tetrasomic inheritance. Theor. Appl. Genet. 100: 1067–1073.
Luo Z.W., Hackett C.A., Bradshaw J.E., McNicol J.W. and Milbourne D. 2001. Construction of a genetic linkage map in tetraploid species using molecular markers. Genetics 157: 1369–1385.
Mackay I.J. and Caligari P.D.S. 2000. Efficiencies of F-2 and backcross generations for bulked segregant analysis using dominant markers. Crop Science 40: 626–630.
Milbourne D., Meyer R.C., Collins A.J., Ramsay L.D., Gebhardt C. and Waugh R. 1998. Isolation, characterisation and mapping of simple sequence repeat loci in potato. Mol. Gen. Genet. 259: 233–245.
Pande B. 2002. The genetic analysis of traits of economic importance in the principal cultivated potato, Solanum tuberosumssp. tuberosum. PhD thesis, University of Dundee, UK.
Phillips M.S. 1980. Screening for resistance to potato cyst nematode using closed containers. Annals of Applied Biology 96: 317–322.
Rouppe van der Voort J.R., van der Vossen E., Bakker E., Overmars H., van Zandroort P., Hutten R., Lankhorst R.K. and Bakker J. 2000. Two additive QTL conferring broad-spectrum resistance in potato to Globodera pallidaare localized on resistance gene clusters. Theor. Appl. Genet. 101: 1122–1130.
Stam P. 1993. Construction of integrated genetic linkage maps by means of a new computer package: JOINMAP. Plant J. 3: 739–744.
Abstract #324_ Keystone Symposium}, La Coeur d'Alene, Idaho, USA, 8-14 February 1999.
Voorrips R.E. 2002. MapChart: Software for the graphical presentation of linkage maps and QTL. The Journal of Heredity 93: 77–78.
Vos P., Hogers R., Bleeker M., Reijans M., Van de Lee T., Hornes M., Frijters A., Pot J., Peleman J., Kuiper M. and Zabeau M. 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 23: 4407–4414.
Wang G.L. and Paterson A.H. 1994. Assessment of DNA Pooling Strategies for Mapping of QTLs. Theor. Appl. Genet. 88: 355–361.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bryan, G.J., McLean, K., Pande, B. et al. Genetical dissection of H3-mediated polygenic PCN resistance in a heterozygous autotetraploid potato population. Molecular Breeding 14, 105–116 (2004). https://doi.org/10.1023/B:MOLB.0000037999.13581.9c
Issue Date:
DOI: https://doi.org/10.1023/B:MOLB.0000037999.13581.9c