Abstract
The adaptation of the methylotrophic bacterium Methylophilus sp. B-7741 to growth in highly deuterated media was studied. For the first time, we showed the cross adaptation of bacterial cells to deuterated media and oxidative and osmotic stresses. The activity of catalase in deuterated cells was higher than in the control cells. Deuterated cell-free culture liquids showed protective effects on the growth of Methylophilus sp. B-7741 in deuterated media, which was manifested as an increase in the deuterated biomass yield. These data and the data available in the literature suggest that the mechanisms of bacterial cell adaptation to heavy water and to oxidative and osmotic stresses are similar.
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REFERENCES
Lobyshev, V.I. and Kalinichenko, L.P., Izotopnye effekty D 2 O v biologicheskikh sistemakh (The Isotope Effects of D2O in Biological Systems), Moscow: Nauka, 1978.
Mosin, O.V., Skladnev, D.A., and Shvets, V.I., Biosynthesis of 2H-Labeled Phenylalanine by a New Mutant of RuMP Facultative Methylotroph Brevibacterium methylicum, Biosci. Biotechnol. Biochem., 1998, vol. 62,no. 2, pp. 225–229.
Kushner, D.J., Baker, A., and Dunstall, T.G., Pharmacological Uses and Perspectives of Heavy Water and Deuterated Compounds, Can. J. Physiol. Pharmacol., 1999, vol. 77, pp. 79–88.
Rastogi, R.P., Skukla, P.C., and Yadava, B., Membrane Permeability of Heavy Water, Biochim. Biophys. Acta, 1971, vol. 249,no. 2, pp. 454–461.
Andjus, P.R. and Vucelic, D., D2O-Induced Cell Excitation, J. Membr. Biol., 1990, vol. 115, pp. 123–127.
Yokogaki, S., Unno, K., Oku, N., and Okada, S., Chaperonin-Repairable Subtle Incompleteness of Protein Assembly Induced by a Substitution of Hydrogen with Deuterium: Effect of GroE on Deuterated Ribulose 1,5-Bisphosphate Carboxylase, Plant Cell Physiol., 1995, vol. 36,no. 3, pp. 419–423.
Brooks, S.C., Osmotic Effects of Deuterium Oxide (Heavy Water) on Living Cells, Science, 1937, vol. 86,no. 2239, pp. 497–498.
Smirnova, G.V., Muzyka, N.G., Glukhovchenko, M.N., and Oktyabr'skii, O.N., Hydrogen Peroxide Modulates Intracellular Levels of Thiols and Potassium in Escherichia coli, Mikrobiologiya, 1998, vol. 67,no. 5, pp. 594–600.
Tkachenko, A.G., Pshenichnov, M.R., Salakhetdinova, O.Ya., and Nesterova, L.Yu., The Role of Putrescine and the Cellular Energy State of Escherichia coli in Regulation of DNA Topology during Adaptation to Oxidative Stress, Mikrobiologiya, 1999, vol. 68,no. 1, pp. 27–32.
Meury, J. and Robin, A., Glutathione-Gated K+ Channels of Escherichia coli Carry Out K+ Efflux Controlled by the Redox State of the Cell, Arch. Microbiol., 1990, vol. 154, pp. 475–482.
Butler, L.O. and Grist, R.W., The Effect of D2O on the Growth and Transforming Activities of Streptococcus pneumoniae, J. Gen. Microbiol., 1984, vol. 130, pp. 483–494.
Nikolaev, Yu.A., Involvement of Exometabolites in Stress Adaptation of Escherichia coli, Mikrobiologiya, 1997, vol. 66,no. 1, pp. 38–41.
Nikolaev, Yu.A., Comparative Study of Two Extracellular Protectants Secreted by Escherichia coli Cells at Elevated Temperatures, Mikrobiologiya, 1997, vol. 66,no. 6, pp. 790–795.
Beers, I.F. and Sizer, W.I., A Spectrophotometric Assay for Measuring the Breakdown of Hydrogen Peroxide by Catalase, J. Biol. Chem., 1952, vol. 196, pp. 133–140.
Smirnova, G.V., Muzyka, N.G., and Oktyabrsky, O.N., The Role of Antioxidant Enzymes in Response of Escherichia coli to Osmotic Upshift, FEMS Microbiol. Lett., 2000, vol. 186, pp. 209–213.
Laser, H. and Slater, E.C., Effect of Heavy Water on Respiratory-Chain Enzymes, Nature (London), 1960, vol. 187,no. 4743, pp. 1115–1117.
Kalinichenko, L.P., Khristova, M.L., and Shnol', S.E., The Effect of D2O, Glycerol, and Aliphatic Alcohols and the Possible Role of Hydrophobic Interactions in Electron Transport in Mitochondria, Biofizika, 1967, vol. 12,no. 5, pp. 824–828.
Siegel, B.Z. and Galston, A.W., Biosynthesis of Deuterated Isoperoxidases in Rye Plants Grown in D2O, Biochemistry, 1966, vol. 56, pp. 1040–1042.
Il'inskaya, O.N., Kolpakov, A.I., Shmidt, M.A., Doroshenko, E.V., Mulyukin, A.L., and El'-Registan, G.I., The Role of Bacterial Growth Autoregulators (Alkyl Hydroxybenzenes) in the Response of Staphylococci to Stresses, Mikrobiologiya, 2002, vol. 71,no. 1, pp. 23–29.
Loiko, N.G., Kozlova, A.N., Osipov, G.A., and El'-Registan, G.I., Low-Molecular-Weight Autoregulatory Factors in the Haloalkaliphilic Bacteria Thioalkalivibrio versutus and Thioalkalimicrobium aerophilum, Mikrobiologiya, 2002, vol. 71,no. 3, pp. 308–315.
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Newo, A.N.S., Pshenichnikova, A.B., Skladnev, D.A. et al. Deuterium Oxide as a Stress Factor for the Methylotrophic Bacterium Methylophilus sp. B-7741. Microbiology 73, 139–142 (2004). https://doi.org/10.1023/B:MICI.0000023979.20916.98
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DOI: https://doi.org/10.1023/B:MICI.0000023979.20916.98