Skip to main content
SpringerLink
Log in

A New Regulatory Function of A-Factor: Stimulation of the Germination of Streptomycete Spores

  • Published:
Microbiology Aims and scope Submit manuscript

Abstract

Spore germination in streptomycetes was shown to be stimulated by exogenously added A-factor. Agar medium either containing or not containing A-factor was inoculated with spore suspensions of three strains differing in their ability to produce regulators of the A-factor group: Streptomyces griseus 773, which produces A-factor and two its lower homologs; S. coelicolor A3(2), which forms six Acl-factors (A-factor analogues); and S. avermitilis JCM5070, which fails to form regulators of this group. A count of the grown colonies showed that exogenous A-factor stimulated spore germination in strains that were themselves able to synthesize regulators of the A-factor group. In S. griseus 773, the number of germinated spores increased by 67% on average after the addition of A-factor to the medium in an amount of 10 μg/ml. In strain S. coelicolor A3 (2), the number of germinated spores increased by 75% after the addition of 1 μg/ml of A-factor. During germination of the S. avermitilis JCM5070 spores, no changes in the CFU number was observed after the addition of A-factor.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. Khokhlov, A.S., Nizkomolekulyarnye mikrobnye autoregulyatory (Low-Molecular-Weight Microbial Autoregulators), Moscow: Nauka, 1988.

    Google Scholar 

  2. Khokhlov, A.S., Microbial Autoregulators, Harwood: Academic, 1991.

    Google Scholar 

  3. Khokhlov, A.S., Tovarova, I.N., Borisova, L.N., Pliner, S.A., Shevchenko, L.A., Kornitskaya, E.Ya., Ivkina, N.S., and Ropoport, I.A., A-Factor Promoting Streptomycin Biosynthesis by a Mutant Strain of Actinomyces streptomycini, Dokl. Akad. Nauk SSSR, 1967, vol. 177,no. 1, pp. 232-235.

    Google Scholar 

  4. Efremenkova, O.V., Anisova, L.N., and Bartoshevich, Yu.E., Regulators of the Differentiation in Actinomycetes (A Review), Antibiot. Med. Biotekhnol., 1985, no. 9, pp. 687-707.

  5. Anisova, L.N., Blinova, I.N., Efremenkova, O.V., Smirnova, G.M., and Khokhlov, A.S., Differentiation Regulators in Streptomyces cyaneofuscatus, Mikrobiologiya, 1984, vol. 53, pp. 890-895.

    Google Scholar 

  6. Kornitskaya, E.Ya., Tovarova, I.I., and Khokhlov, A.S., A Study of the Production of A-Factor by Various Actinomycetes, Mikrobiologiya, 1976, vol. 45, pp. 302-305.

    Google Scholar 

  7. Efremenkova, O.V., Anisova, L.N., and Khokhlov, A.S., Excretion by Various Actinomycetes of Compounds Inducing Sporulation in an Asporogenous Mutant of Streptomyces griseus, Mikrobiologiya, 1979, vol. 48, pp. 999-1003.

    Google Scholar 

  8. Eritt, I., Gräfe, U., and Fleck, W.F., A Screening Method for Autoregulators of Anthracycline-Producing Streptomycetes, Z. Allg. Microbiol., 1982, vol. 22, pp. 91-96.

    Google Scholar 

  9. Efremenkova, O.V., Anisova, L.N., Kamzolkina, O.V., Dmitrieva, S.V., Gorin, S.E., and Bartoshevich, Yu.E., Production of A-Factor-Type Regulators by representatives of the genus Micromonospora, Antibiot. Med. Biotekhnol., 1987, vol. 32,no. 9, pp. 643-648.

    Google Scholar 

  10. Bushueva, O.A., Efremenkova, O.V., Gorin, S.E., and Bartoshevich, Yu.E., The Effect of Low-Molecular-Weight Regulators on the Biosynthesis of Rifamycin B by Amycolatopsis mediterranei Strains, Antibiot. Khimioter., 1991, vol. 36,no. 3, pp. 11-15.

    Google Scholar 

  11. Borisova, L.N., Ivkina, N.S., and Gomenyuk, L.A., A Study of Complementary Combinations of Secondary Biosynthetic Mutants of Actinomyces streptomycini, Dokl. Akad. Nauk SSSR, 1970, vol. 192, pp. 199-202.

    Google Scholar 

  12. Lakin, G.F., Biometriya (Biometrics), Moscow: Nauchnaya Shkola, 1990.

    Google Scholar 

  13. Kleiner, E.N., Pliner, S.A., Soifer, V.S., Onoprienko, V.V., Balashova, T.A., Rozynov, B.V., and Khokhlov, A.S., Structure of A-Factor, a Bioregulator from Streptomyces griseus, Bioorg. Khim., 1976, vol. 2, pp. 1142-1147.

    Google Scholar 

  14. Anisova, L.N., Blinova, I.N., Efremenkova, O.V., Koz'min, Yu.P., Onoprienko, V.V., Smirnova, G.N., and Khokhlov, A.S., Regulators of the Development of Streptomyces coelicolor A3(2), Izv. Akad. Nauk SSSR, Ser. Biol., 1984, pp. 98-108.

  15. Efremenkova, O.V., Gruzina, V.D., Sumarukova, I.G., and Kuznetsov, V.D., Search for A-Factor-Dependent Variants in Actinomycete Populations, Mikrobiologiya, 2003, vol. 72,no. 6, pp. 766-779.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Gause Institute of New Antibiotics, Russian Academy of Medical Sciences, ul. Bol'shaya Pirogovskaya 11, Moscow, 119021, Russia

    V. D. Gruzina, O. V. Efremenkova & Yu. V. Dudnik

  2. Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117312, Russia

    E. V. Gorbatyuk, S. N. Filippova & G. I. El'-Registan

Authors
  1. V. D. Gruzina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. V. Gorbatyuk
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. O. V. Efremenkova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. N. Filippova
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. G. I. El'-Registan
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yu. V. Dudnik
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gruzina, V.D., Gorbatyuk, E.V., Efremenkova, O.V. et al. A New Regulatory Function of A-Factor: Stimulation of the Germination of Streptomycete Spores. Microbiology 72, 682–685 (2003). https://doi.org/10.1023/B:MICI.0000008369.13070.3c

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/B:MICI.0000008369.13070.3c

Search

Navigation