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Search for A-factor-Dependent Variants in Actinomycete Populations

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Abstract

A study of 28 nocardia-like, asporogenous, and oligosporous spontaneous morphological variants belonging to 23 species of streptomycetes revealed five strains producing regulators of the A-factor group. Streptomyces griseus 1439, which forms aerial mycelium and spores only in the presence of exogenous A-factor, was used as the test strain. Among the 28 spontaneous variants, three new A-factor-dependent strains were revealed, which represented the species Streptomyces griseus, “S. citreofluorescens,” and “S. viridovulgaris subsp. albomarinus.” These weakly differentiated variants did not produce A-factor and behaved as its recipients, responding by changes in their morphological characteristics at a concentration of this regulator in the medium of 0.01 μg/ml or higher. The original collection strains in whose populations the variants were selected produced substances of the A-factor group. The A-factor-dependent variants differed in the level of the regulator required for maximal expression of the morphological characteristics: it was necessary to introduce the A-factor at a concentration of 1 μg/ml for “S. citreofluorescens” and “S. viridovulgaris subsp. albomarinus” and at 10 μg/ml for S. griseus.

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Authors and Affiliations

  1. Gause Institute of New Antibiotics, Russian Academy of Medical Sciences, ul. Bol'shaya Pirogovskaya 11, Moscow, 119021, Russia

    O. V. Efremenkova, V. D. Gruzina & I. G. Sumarukova

  2. Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117312, Russia

    V. D. Kuznetsov

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  1. O. V. Efremenkova
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  2. V. D. Gruzina
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  3. I. G. Sumarukova
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  4. V. D. Kuznetsov
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Efremenkova, O.V., Gruzina, V.D., Sumarukova, I.G. et al. Search for A-factor-Dependent Variants in Actinomycete Populations. Microbiology 72, 678–681 (2003). https://doi.org/10.1023/B:MICI.0000008368.76532.1f

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  • DOI: https://doi.org/10.1023/B:MICI.0000008368.76532.1f

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