Abstract
Ultraviolet-B (UV-B) irradiation in the range of 280–320nm has shown to be a promising immunomodulatory tool in xenogenic hepatocyte transplantation. Most of the studies documenting the effect(s) of UV-B irradiation on hepatic transplantation have been carried out in small model systems with very little information available in larger animals. The aim of the present investigation was to study in vitro the effect(s) of UV-B irradiation (302 nm) at 0, 250, 500, 1250 and 2500 J/m2 on the viability and cellular responses in the isolated goat hepatocytes. The results showed that the cells irradiated at 0, 250, 500, 1250 and 2500 J/m2 demonstrated a viability of 90–95%. However, intracellular [Ca2+]i influx as quantitated by Flu 3-acetete showed a significant increase with irradiation as observed in confocal microscope. The intracellular pH (quantitated by the flourescence of BCCEF) although tend to show an increase with UV-B irradiation was not statistically significant. The present observations suggest that there is a modulation in the intracellular [Ca2+]i concentration within the hepatocytes at higher dose of UV-B irradiation without altering the viability of hepatocytes. These observations are significant for the xenotransplantation of cells (Mol Cell Biochem 266: 161–166, 2004)
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Auchincloss Jr H, Sachs DH: Xenogeneic transplantation. Annu Rev Immunol 16: 433–470, 1998
Bach FH, Winkler H, Ferran C, Hancock WW, Robson SC: Delayed xenograft rejection Immunol Today 17: 379–384, 1996
Galili U: The β-gal epitope (Galβ 1–3Galβ 1–4GlcNAc-R) in xenotrans-plantation. Biochemie 83: 557–563, 2000
Deeg HJ: Ultraviolet irradiation in transplantation biology. Manipulation of immunity and immunogenecity Transplantation 45: 845–851 1988
Tze WJ, Cheung S, Tai J, Tsang A: Xenotransplantation of adult porcine islets in diabetic mice. A study of UV-B irradiation, cryopreservation and immunosuppresion on graft survival time. Horm Metab Res 30: 509–513, 1988
Kripke ML: Immunogical unresponsiveness induced by ultraviolet irradiation. Immunol Rev 80: 87–93, 1984
Lahiri S, Habibullah CM, Ayesha Q, Khan AA, Srinivas VK, Naithani R: Effect of UV-B (302 nm) irradiation on isolated rat hepatocytes. Liver 15: 149–152, 1995
Habibullah CM, Ayesha Q, Khan AA, Naithani R, Lahiri S. Xeno-transplantation of UV-B irradiated hepatocytes-survival and immune response. Transplantation 59: 1495–1497, 1995
Fulton KL, Clarke, Farris Jr EH: Farm animals in biomedical research Part 2. The goat as a model for biomedical research and teaching. ILAR News, Institute of Laboratory Animal Resources, 1994, pp 21–29
Patience C, Takeuchi Y, Weiss KA: Infection of human cells by an endogenous retrovirus of pigs. Nat Med 3: 282–286, 1997
Lincicome P, Hall A: The pygmy. In: F.W. George, Haenlein, L. Donald, Ace (eds). Extention Goat Handbook. Coperative Extension Service, University of Deaware, Newark, Deaware, 1984, pp 1–4
Habibullah CM, Hassan SI, Ayesha Q, Zaman T, Mukarram BM, Devi G. Fetal liver perfusion, a new approach. Surg Res Commun 8: 87–90 1990
Jauregui HO, Hayner NT, Driscoll JL, Williams-Holland R, Lipsky JL, Galletti PM: Trypan blue dye uptake and lactate dehydrogenase in adult rat hepatocytes; freshly isolated cells, cell suspensions, and primary monolayer. In Vitro 17: 1100–1110, 1981
Mossmann T: Rapid colorimetric assay for cellular growth and survival; application to proliferation and cytotoxicity assays. J Immunol Methods 65: 55–63, 1983
Sawas AH, Gilbert JC: Effect of adrenergic, agonists and antogonists and the catechol nucleus on the Na + K+ ATPase and Mg+ ATPase activities of synaptosomes. Biochem Pharmacol 30: 1799–1803, 1981
Raghuramulu N, Madhavan NK, Kalyanasundaram N. In: N. Raghu-ramulu, N.K. Madhavan, S. Kalyanasundaram (eds). Manual of Laboratory Techniques. National Institute of Nutrition, Indian Council of Medical Research, India, 1983, pp 141–142
Haugland RP. In: T.Z. Michelle, L.D. Spence, L.D. Johnson (eds). Handbook of Fluorescent Probes and Research Chemicals, 6th edn. Molecular Probes Inc., 1996, pp 511–512.
Haugland RP. In: T.Z. Michelle, L.D. Spence, L.D. Johnson (eds). Handbook of Fluorescent Probes and Research Chemicals, 6th edn. Molecular Probes Inc., 1996, pp 553–555
Lanza RP, Cooper DKC: Xenotransplantation of cells and tissues; application to a range of diseases from diabetes to Alzheimers Mol Med Today 4: 39–40, 1998
Habibullah CM: An overviewof hepatocyte transplantation. In: M. Mito, M. Sawas (eds). Hepatocyte Transplantation. Karger Landes Systems, Japan, 1997, pp 312–324
Pamphillon DH, Alnaqdy AA, Wallington TB: Immunomodulation by ultraviolet light. Clinical studies and biological effects. Immunol Today 12: 119–123, 1991
Sun J, Wang L, Waring MA, Wang C, Woodman KK, Sheil AG: Simple and reliable methods to assess hepatocyte viability in bio-artificial liver support system matrices. Artif Organs 21: 408–413, 1997
Bay BH: Extracellular ATP induces cytosolic calcium oscillations associated with an increase in intracellular pH in human chang liver cells.Cell Mol Biol 43:521–528, 1997
Grinstein S, Rotin D, Mason MJ: Na+/H+ exchange and growth factor induced cytosolic pH changes. Role in cellular proliferation. Biochem Biophys Acta 988: 73–97, 1989
Sit KH, Bay BH, Wong KP: Extracellular ATP induces rapid cell rounding in cultured Chang liver cells. Jpn J Physiol 42: 355–362, 1992
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Venkateshan, V., Shakeel, N.B., Rao, N.M. et al. Differential responses of UV-B irradiation on the viability and intracellular calcium influx in goat hepatocytes–in vitro effect. Mol Cell Biochem 266, 161–166 (2004). https://doi.org/10.1023/B:MCBI.0000049155.32426.10
Issue Date:
DOI: https://doi.org/10.1023/B:MCBI.0000049155.32426.10