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Ventricular but not atrial electro-mechanical delay of the embryonic heart is altered by anoxia-reoxygenation and improved by nitric oxide

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Abstract

Background/Aim. Excitation–contraction coupling is modulated by nitric oxide (NO) which otherwise has either beneficial or detrimental effects on myocardial function during hypoxia-reoxygenation. This work aimed at characterizing the variations of electromechanical delay (EMD) induced by anoxia-reoxygenation within the developing heart and determining whether atrial and ventricular EMD are modulated by NO to the same extent. Methods. Hearts of 4 or 4.5-day-old chick embryos were excised and submitted in vitro to normoxia (45 min), anoxia (30 min) and reoxygenation (60 min). Electrocardiogram and atrial and ventricular contractions were simultaneously recorded throughout experiment. Anoxia-reoxygenation-induced chrono-, dromo- and inotropic disturbances and changes in EMD in atrium (EMDa) and ventricle (EMDv) were investigated in control hearts and in hearts exposed to 0.1, 1, 10, 50 and 100 μM of DETA-NONOate (a NO donating agent) or to 50 μM of L-NAME (a NOS inhibitor). Results. Under normoxia, heart rate, PR interval, ventricular shortening velocity, EMDa and EMDv were similar in control, L-NAME-treated and DETA-NONOate-treated hearts. Under anoxia, cardiac activity became markedly erratic within less than 10 min in all groups. At the onset of reoxygenation, EMDv was increased by about 300% with respect to the preanoxic value while EMDa did not vary significatively. Compared to control conditions, L-NAME or DETA-NONOate had no influence on the negative chrono-, dromo- and inotropic effects induced by anoxia-reoxygenation. However, L-NAME prolonged EMDv during anoxia and delayed EMDv recovery during reoxygenation while 100 μM DETA-NONOate had the opposite effects. EMDa was neither affected by NOS inhibitor nor NO donor. At the end of reoxygenation, all the investigated parameters returned to their basal values. Conclusion. This work provides evidence that a NO-dependent pathway is involved in regulation of the ventricular excitation-contraction coupling in the anoxic-reoxygenated developing heart. (Mol Cell Biochem 265: 141–149, 2004)

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Authors and Affiliations

  1. Division of Cardiology, University Hospital, Lausanne, Switzerland; Institute of Physiology, Faculty of Medicine, Lausanne, Switzerland

    Philippe Maury, Antonio Rosa & Lukas Kappenberger

  2. Faculty of Medicine, Institute of Physiology, Lausanne, Switzerland

    Alexandre Sarre, Jérôme Terrand, Pavel Kucera & Eric Raddatz

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Maury, P., Sarre, A., Terrand, J. et al. Ventricular but not atrial electro-mechanical delay of the embryonic heart is altered by anoxia-reoxygenation and improved by nitric oxide. Mol Cell Biochem 265, 141–149 (2004). https://doi.org/10.1023/B:MCBI.0000044391.97857.4d

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