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Altered Mg2+ transport across liver plasma membrane from streptozotocin-treated rats

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Abstract

Type-I diabetes is associated with a decrease in magnesium content in various tissues, including liver. We have reported that hepatocytes from streptozotocin-injected rats have lost the ability to accumulate Mg2+ following hormonal stimulation. To assess whether the defect is inherent to the Mg2+ transport mechanism located in the hepatocyte cell membrane, plasma membrane vesicles were purified from diabetic livers. Diabetic plasma membranes do not retain intravesicular Mg2+as tightly as vesicles purified from livers of age-matched non-diabetic rats. In addition, the amount of intravesicular Mg2+ these vesicles exchange for extravesicular Na+ or Ca2+ is 2–3-fold larger than in non-diabetic vesicles. The partition of Ca2+/Mg2+ and Na+/Mg2+ exchange mechanisms in the apical and basolateral domains of liver plasma membrane is maintained under diabetic conditions, although the Na+/Mg2+ exchanger in diabetic basolateral membranes has lost the ability to operate in reverse and favor an accumulation of extravesicular Mg2+ within the vesicles in exchange for entrapped Na+. These data indicate the occurrence of a major alteration in Mg2+ transport across the hepatocyte membrane, which can explain, at least in part, the decrease in liver magnesium content observed in diabetic animals and patients. (Mol Cell Biochem 262: 145–154, 2004)

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Authors and Affiliations

  1. Department of Physiology and Biophysics, School of Medicine, Case Western Reserve University, Cleveland, OH, USA

    Christie Cefaratti & Amy McKinnis

  2. Department of Physiology and Biophysics, School of Medicine, Case Western Reserve University, Cleveland, OH, USA

    Andrea Romani

Authors
  1. Christie Cefaratti
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  2. Amy McKinnis
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  3. Andrea Romani
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Cefaratti, C., McKinnis, A. & Romani, A. Altered Mg2+ transport across liver plasma membrane from streptozotocin-treated rats. Mol Cell Biochem 262, 145–154 (2004). https://doi.org/10.1023/B:MCBI.0000038230.86485.52

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  • DOI: https://doi.org/10.1023/B:MCBI.0000038230.86485.52

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