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Regulation of protein kinase C isozymes in volume overload cardiac hypertrophy

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Abstract

Protein kinase C (PKC) is a family of at least 11 isozymes and known to play a crucial role in myocardial growth. The present study was performed to investigate whether PKC-isozymes are differentially regulated during the development of volume-overload cardiac hypertrophy. After 2, 7 and 30 days of sham or aortocaval shunt operation in male Wistar rats, PKC-activity and the expression of cardiac PKC-isozymes (PKC-α, δ and ε) were determined at the protein and at the mRNA-level in the left and the right ventricle separately. Myocardial hypertrophy after 2, 7 and 30 days of aortocaval shunt was more pronounced in the right than in the left ventricle. Right ventricular hypertrophy was associated with an increased PKC-enzyme activity, a selectively enhanced protein expression of cytosolic PKC-δ (day 7:+83 ± 12%, day 30:+94 ± 14%) and PKC-α (day 7:+48 ± 11%, day 30:+62 ± 16%) and a transcriptional upregulation of the absolute mRNA-levels of these PKC-isozymes in the aortocaval shunt group as compared to controls. In contrast, the expression of PKC-ε was unchanged. A significant upregulation of PKC-δ both on the protein and on the mRNA-level was also noted in volume-overload induced left ventricular hypertrophy, whereas the expression of PKC-α and PKC-ε were not altered. Furthermore, the expression of calcineurin in both ventricles was not significantly changed in response to volume-overload. This study characterizes in the left and right ventricle a differential regulation of the dominant PKC-isozymes in volume-overload cardiac hypertrophy both at the protein and at the mRNA-level. (Mol Cell Biochem 262: 135–143, 2004)

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Authors and Affiliations

  1. Department of Cardiology, Medical Clinic II, University of Technology Dresden, Germany

    Martin U. Braun

  2. Department of Cardiology, Angiology and Pulmonology, Medical Clinic III, University of Heidelberg, Germany

    Paul LaRosée & Mathias M. Borst

  3. Department of Cardiology, Medical Clinic II, University of Technology Dresden, Germany

    Gregor Simonis & Ruth H. Strasser

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  1. Martin U. Braun
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  2. Paul LaRosée
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  3. Gregor Simonis
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  4. Mathias M. Borst
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  5. Ruth H. Strasser
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Braun, M.U., LaRosée, P., Simonis, G. et al. Regulation of protein kinase C isozymes in volume overload cardiac hypertrophy. Mol Cell Biochem 262, 135–143 (2004). https://doi.org/10.1023/B:MCBI.0000038229.23132.9f

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  • DOI: https://doi.org/10.1023/B:MCBI.0000038229.23132.9f

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