Abstract
Mechanical forces related to pressure and flow are important for cell hypertrophy and proliferation. There are still a few studies that examine responses of human vascular smooth muscle cells to pure pressure stress (transmural pressure). It is unclear as to which mechanisms are involved in cellular responses to pressure elevation. On the other hand, although the involvement of the local renin-angiotensin system (RAS) in pressure-induced responses was reported, the results were contradictory. It still remains to be determined whether RAS in human vascular smooth muscle cells is activated by pure pressure stress. We studied the upstream signal transduction events of extracellular signal kinase (ERK) in response to atmospheric pressure stress and involvement of angiotensin II in pressure-induced cell proliferation in human aortic smooth muscle cells (HASMC). A pressure-loading apparatus was set up to examine the effects of atmospheric pressure on human aortic smooth muscle cells. Pressure application of 160 mmHg for 3 h produced cell proliferation and activated ERK and c-JUN N-terminal kinase (JNK). ACE inhibitor suppressed all of them. ERK kinase (MEK) inhibitor also suppressed cell proliferation stimulated by pure pressure. The phosphorylated c-Src was increased by pure pressure stress. The treatment with c-Src kinase inhibitor suppressed pressure-induced proliferative response. In summary, our study found that ERK activation mediated pure pressure-induced proliferative response of HASMC. This activation was partly mediated by c-Src. (Mol Cell Biochem 262: 71–78, 2004)
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Malek AM, Alper SL, Izumo S: Hemodynamic shear stress and its role in atherosclerosis. JAMA 282: 2035–2042, 1999
Iizuka K, Murakami T, Kawaguchi H: Pure atmospheric pressure promotes an expression of osteopontin in human aortic smooth muscle cells. Biochem Biophys Res Commun 283: 493–498, 2001
Yasunari K, Maeda K, Nakamura M, Yoshikawa J: Pressure promotes angiotensin II-mediated migration of human coronary smooth muscle cells through increase in oxidative stress. Hypertension 39: 433–437, 2002
Ozaki T, Iizuka K, Suzuki M, Murakami T, KitabatakeA, Kawaguchi H: Threshold-dependent DNA synthesis by pure pressure in human aortic smooth muscle cells: Gi alpha-dependent and-independent pathways. Biochem Biophys Res Commun 256: 212–217, 1999
Tsuda Y, Okazaki M, Uezono Y, Osajima A, Kato H, Okuda H, Oishi Y, Yashiro A, Nakashima Y: Activation of extracellular signal-regulated kinases is essential for pressure-induced proliferation of vascular smooth muscle cells. Eur J Pharmacol 446: 15–24, 2002
Hishikawa K, Nakaki T, Marumo T, Hayashi M, Suzuki H, Kato R, Saruta T: Pressure promotes DNA synthesis in rat cultured vascular smooth muscle cells. J Clin Invest 93: 1975–1980, 1994
Kawata Y, Mizukami Y, Fujii Z, Sakumura T, Yoda K, Matsuzaki M: Applied pressure enhances cell proliferation through mitogen-activated protein kinase activation in mesangial cells. J Biol Chem 273: 16905–16912, 1998
Hishikawa K, Nakaki T, Marumo T, Suzuki H, Kato R, Saruta T: Pressure enhances endothelin-1 release from cultured human endothelial cells. Hypertension 25: 449–452, 1995
Kubo T, Hosokawa H, Kambe T, Fukumori R: Angiotensin II mediates pressure loading-induced mitogen-activated protein kinase activation in isolated rat aorta. Eur J Pharmacol 391: 281–287, 2000
Kageyama K, Murakami T, Iizuka K, Sato K, Ichihara K, Tokumitsu Y, Kitabatake A, Kawaguchi H: Translocation of G protein β3 subunit from the cytosol pool to the membrane pool by β1-adrenergic receptor stimulation in perfused rat hearts. Biochem Pharmacol 58: 149–1500, 1999
Urasawa K, Yoshida I, Takagi C, Onozuka H, Mikami T, Kawaguchi H, KitabatakeA: Enhanced Expression of β-adrenergic receptor kinase 1 in the hearts of cardiomyopathic hamsters, BIO 53.58. Biochem Biophys Res Commun 219: 26–30, 1996
Dudley DT, Pang L, Decker SJ, Bridges AJ, Saltiel AR: A synthetic inhibitor of the mitogen-activated protein kinase cascade. Proc Natl Acad Sci USA 92: 7686–7689, 1995
Alessi DR, Cuenda A, Cohen P, Dudley DT, Saltiel AR: PD 098059 is a specific inhibitor of the activation of mitogen-activated protein kinase kinase in vitro and in vivo. J Biol Chem 270: 27489–27494, 1995
Li C, Xu Q: Mechanical stress-initiated signal transductions in vascular smooth muscle cells. Cell Signal 12: 435–445, 2000
Murasawa S, Matsubara H, Mori Y, Masaki H, Tsutsumi Y, Shibasaki Y, Kitabayashi I, Tanaka Y, Fujiyama S, Koyama Y, Fujiyama A, Iba S, Iwasaka T: Angiotensin II initiates tyrosine kinase Pyk2-dependent signalings leading to activation of Rac1-mediated c-Jun NH2-terminal kinase. J Biol Chem 275: 26856–26863, 2000
Rocic P, Govindarajan G, Sabri A, Lucchesi PA: A role for PYK2 in regulation of ERK1/2 MAP kinases and PI 3-kinase by ANG II in vascular smooth muscle. Am J Physiol Cell Physiol 280: C90–C99, 2000
Ishigai Y, Mori T, Ikeda T, Fukuzawa A, Shibano T: Role of bradykinin-NO pathway in prevention of cardiac hypertrophy by ACE inhibitor in rat cardiomyocytes. Am J Physiol 273: H2659–H2663, 1997
Saito S, Frank GD, Mifune M, Ohba M, Utsunomiya H, Motley ED, Inagami T, Eguchi S:Ligand-independent trans-activation of the platelet-derived growth factor receptor by reactive oxygen species requires protein kinase C-delta and SRC. J Biol Chem 277: 44695–44700, 2000
Robin P, Boulven I, Desmyter C, Harbon S, Leiber D: ET-1 stimulates ERK signaling pathway through sequential activation of PKC and Src in rat myometrial cells. Am J Physiol Cell Physiol 283: C251–C260, 2002
Seshiah PN, Weber DS, Rocic P, Lalppu V, Taniyama Y, Griendling K: Angiotensin II stimulation of NAD(P)H oxidase activity: Upstream mediators. Circ Res 91: 406–413, 2002
Oeckler RA, Kaminski PM, Wolin MS: Stretch enhances contraction of bovine coronary arteries via an NAD(P)H oxidase-mediated activation of the extracellular signal-regulated kinase mitogen-activated protein kinase cascade. Circ Res 92: 23–31, 2003
Davis ME, Cai H, Drummond GR, Harrison DG: Shear stress regulates endothelial nitric oxide synthase expression through c-Src by divergent signaling pathways. Circ Res 89: 1073–1080, 2001
Yamazaki T, Komuro I, Zou Y, Yazaki Y: Hypertrophic responses of cardiomyocytes induced by endothelin-1 through the protein kinase Cdependent but Src and Ras-independent pathways. Hypertens Res 22: 113–119, 1999
Rosenblum K, Futter M, Jones M, Hulme EC, Bliss TV: ERKI/II regulation by the muscarinic acetylcholine receptors in neurons. J Neurosci 20: 977–985, 2000
Sano H, Okamoto H, Kitabatake A, Iizuka K, Murakami T, Kawaguchi H: Increased mRNA expression of cardiac renin-angiotensin system and collagen synthesis in spontaneously hypertensive rats. Mol Cell Biochem 178: 51–58, 1998
Watanabe M, Kawaguchi H, Onozuka H, Mikami T, Urasawa K, Okamoto H, Watanabe S, Abe K, Kitabatake A: The chronic effect of enalapril and amlodipine on cardiac remodeling in cardiomyopathic hamster hearts: Ca2+ antagonist and ACEI in Cardiomyopathy. J Cardiovasc Pharmacol 32: 248–259, 1998
StanleyAG, Patel H, Knight AL, Williams B: Mechanical strain-induced human vascular matrix synthesis: The role of angiotensin II. J Renin Angiotensin Aldosterone Syst 1: 32–35, 2000
Tamura K, Chen YE, Chen Q, Nyui N, Horiuchi M, Takasaki I, Tamura N, Pratt RE, Dzau VJ, Umemura S: Expression of renin-angiotensin system and extracellular matrix genes in cardiovascular cells and its regulation through AT1 receptor. Mol Cell Biochem 212: 203–209, 2000
Gosgnach W, Challah M, Coulet F, Michel JB, Battle T: Shear stress induces angiotensin converting enzyme expression in cultured smooth muscle cells: Possible involvement of bFGF. Cardiovasc Res 45: 486–492, 2000
Paul M, Wagner J, Dzau VJ: Gene expression of the renin-angiotensin system in human tissues. Quantitative analysis by the polymerase chain reaction. J Clin Invest 91: 2058–2064, 1993
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Morita, N., Iizuka, K., Murakami, T. et al. N-terminal kinase, and c-Src are activated in human aortic smooth muscle cells by pressure stress. Mol Cell Biochem 262, 71–78 (2004). https://doi.org/10.1023/B:MCBI.0000038218.09259.1c
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DOI: https://doi.org/10.1023/B:MCBI.0000038218.09259.1c