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Effects of acidosis on ventricular myocyte shortening and intracellular Ca2+ in streptozotocin-induced diabetic rats

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Abstract

We have investigated the effects of acute acidosis on ventricular myocyte shortening and intracellular Ca2+ in streptozotocin (STZ)-induced diabetic rat. Shortening and intracellular Ca2+ were measured in electrically stimulated myocytes superfused with either normal Tyrode solution pH adjusted to either 7.4 (control solution) or 6.4 (acid solution). Experiments were performed at 35–36°C. At 8–12 weeks after treatment, the rats that received STZ had lower body and heart weights compared to controls, and blood glucose was characteristically increased. Contractile defects in myocytes from diabetic rat were characterized by prolonged time to peak shortening. Superfusion of myocytes from control and diabetic rats with acid solution caused a significant reduction in the amplitude of shortening; however, the magnitude of the response was not altered by STZ treatment. Acid solution also caused significant and quantitatively similar reductions in the amplitude of Ca2+ transients in myocytes from control and diabetic rats. Effects of acute acidosis on amplitude of myocyte contraction and Ca2+ transient were not significantly altered by STZ treatment. Altered myofilament sensitivity to Ca2+ and altered mechanisms of sarcoplasmic reticulum Ca2+ transport might partly underlie the acidosis-evoked reduction in amplitude of shortening in myocytes from control and STZ-induced diabetic rat. (Mol Cell Biochem 261: 227–233, 2004)

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References

  1. Dhalla NS, Pierce GN, Innes IR, Beamish RE: Pathogenesis of cardiac dysfunction in diabetes mellitus. Can J Cardiol 1: 263–281, 1985

    CAS  PubMed  Google Scholar 

  2. Choi KM, Zhong Y, Hoit BD, Grupp IL, Hahn H, Dilly KW, Guatimosim S, Lederer WJ, Matlib MA: Defective intracellular Ca(2+) signaling contributes to cardiomyopathy in type 1 diabetic rats. Am J Physiol 283: H1398–H1408, 2002

    CAS  Google Scholar 

  3. Howarth FC, Qureshi MA, Bracken NK, Winlow W, Singh J: Time-dependent effects of streptozotocin-induced diabetes on contraction of ventricular myocytes from rat heart. Em Med J 19: 35–41, 2001

    Google Scholar 

  4. Yu Z, Tibbits GF, Mcneill JH: Cellular functions of diabetic cardiomyocytes: Contractility, rapid-cooling contracture, and ryanodine binding. Am J Physiol 266: H2082–H2089, 1994

    CAS  PubMed  Google Scholar 

  5. Ren J, Davidoff AJ: Diabetes rapidly induces contractile dysfunctions in isolated ventricular myocytes. Am J Physiol 41: H148–H158, 1997

    Google Scholar 

  6. Hattori Y, Matsuda N, Kimura J, Ishitani T, Tamada A, Gando S, Kemmotsu O, Kanno M: Diminished function and expression of the cardiac Na+-Ca2+ exchanger in diabetic rats: Implication in Ca2+ overload. J Physiol 527: 85–94, 2002

    Google Scholar 

  7. Hulme JT, Orchard CH: Effect of acidosis on Ca2+ uptake and release by sarcoplasmic reticulum of intact rat ventricular myocytes. Am J Physiol 275: H977–H987, 1998

    CAS  PubMed  Google Scholar 

  8. Bountra C, Vaughan-Jones RD: Effect of intracellular and extracellular pH on contraction in isolated, mammalian cardiac muscle. J Physiol 418: 163–87, 1989

    CAS  PubMed  Google Scholar 

  9. Harrison SM, Frampton JE, McCall E, Boyett MR, Orchard CH: Contraction and intracellular Ca2+, Na+, and H+ during acidosis in rat ventricular myocytes. Am J Physiol 262: C348–C357, 1992

    CAS  PubMed  Google Scholar 

  10. Orchard CH, Kentish JC: Effects of changes of pH on the contractile function of cardiac muscle. Am J Physiol 258: C967–C981, 1990

    CAS  PubMed  Google Scholar 

  11. Lagadic-Gossmann D, Le Prigent K, Baut GL, Caignard DH, Renard P, Scalbert E, Feuvray D: Effects of S20787 on pHi-regulating mechanisms in isolated rat ventricular myocytes. J Cardiovasc Pharmacol 28: 547–552, 1996

    Article  CAS  PubMed  Google Scholar 

  12. Wallert MA, Frohlich O: Na+-H+exchange in isolated myocytes from adult rat heart. Am J Physiol 257: C207–C213, 1989

    CAS  PubMed  Google Scholar 

  13. Lagadic-Gossmann D, Chesnais JM, Feuvray D: Intracellular pH regulation in papillary muscle cells from streptozotocin diabetic rats: An ion-sensitive microelectrode study. Pflügers Arch 412: 613–617, 1988

    Article  CAS  PubMed  Google Scholar 

  14. Pierce GN, Ramjiawan B, Dhalla NS, Ferrari R: Na(+)-H+exchange in cardiac sarcolemmal vesicles isolated from diabetic rats. Am J Physiol 258: H255–H261, 1990

    CAS  PubMed  Google Scholar 

  15. Howarth FC, Qureshi MA, White E: Effects of hyperosmotic shrinking on ventricular myocyte shortening and intracellular Ca(2+) in streptozotocin-induced diabetic rats. Pflügers Arch 444: 446–451, 2002

    CAS  PubMed  Google Scholar 

  16. White E, Boyett MR, Orchard CH: The effects of mechanical loading and changes of length on single guinea-pig ventricular myocytes. J Physiol 482: 93–107, 1995

    CAS  PubMed  Google Scholar 

  17. Baandrup U, Ledet T, Rasch R: Experimental diabetic cardiopathy preventable by insulin treatment. Lab Invest 45: 169–173, 1981

    CAS  PubMed  Google Scholar 

  18. Vanheel B, de Hemptinne A, Leusen I: Influence of surface pH on intracellular pH regulation in cardiac and skeletal muscle. Am J Physiol 250: C748–C760, 1986

    CAS  PubMed  Google Scholar 

  19. Wu ML, Vaughan-Jones RD: Inhibition of Na+/H+ exchange in the sheep Purkinje fibre. J Mol Cell Cardiol 20(suppl IV): S.47, 1988

    Google Scholar 

  20. Allen DG, Orchard CH: The effects of changes of pH on intracellular calcium transients in mammalian cardiac muscle. J Physiol 335: 555–567, 1983

    CAS  PubMed  Google Scholar 

  21. Sham JSK, Cleemann L, Morad M: Role of Na+ channel and Na+-Ca2+ exchanger in the release of Ca2+ in cardiomyocytes. FASEB J 6: A26, 1992

    Google Scholar 

  22. Lipp P, Niggli E: Sodium current-induced calcium signals in isolated guinea-pig ventricular myocytes. J Physiol 474: 439–446, 1994

    CAS  PubMed  Google Scholar 

  23. Sham JSK, Cleemann L, Morad M: Gating of the cardiac Ca2+ release channel: The role of Na+ current and Na+-Ca+ exchange. Science 255: 850–853, 1992

    CAS  PubMed  Google Scholar 

  24. Levi AJ, Brooksby P, Hancox JC: One hump or two?—The triggering of calcium release from the sarcoplasmic reticulum and the voltage-dependence of contraction in mammalian cardiac muscle. Cardiovasc Res 27: 1743–1757, 1993

    CAS  PubMed  Google Scholar 

  25. Fabiato A: Calcium-induced release of calcium from the cardiac sarcoplasmic reticulum. Am J Physiol 14: C1–C14, 1983

    Google Scholar 

  26. Cannell MB, Berlin JR, Lederer WJ: Intracellular calcium in cardiac myocytes: Calcium transients measured using fluorescence imaging. In: L.J. Mandel, D.C. Eaton (eds). Cell Calcium and the Control of Membrane Transport. The Rockefeller University Press, New York, 1987, pp 201–214

    Google Scholar 

  27. Fry CH, Poole-Wilson PA: Effects of acid-base changes on excitation-contraction coupling in guinea-pig and rabbit cardiac ventricular muscle. J Physiol 313: 141–60, 1981

    CAS  PubMed  Google Scholar 

  28. Kohlhardt M, Haap K, Figulla HR: Influence of low extracellular pH upon the Ca inward current and isometric contractile force in mammalian ventricular myocardium. Pflügers Arch 366: 31–38, 1976

    CAS  PubMed  Google Scholar 

  29. Vogel S, Sperelakis N: Blockade of myocardial slow inward current at low pH. Am J Physiol 233: C99–C103, 1977

    CAS  PubMed  Google Scholar 

  30. Earm YE, Irisawa H: Effects of pH on the Na-Ca exchange current in single ventricular cells of the guinea pig. Jpn Heart J 27(suppl 1): 153–158, 1986

    PubMed  Google Scholar 

  31. Philipson KD, Bersohn MM, Nishimoto AY: Effects of pH on Na+-Ca2+ exchange in canine cardiac sarcolemmal vesicles. Circ Res 50: 287–293, 1982

    CAS  PubMed  Google Scholar 

  32. Rousseau E, Pinkos J: pH modulates conducting and gating behaviour of single calcium release channels. Pflügers Arch 415: 645–647, 1990

    CAS  PubMed  Google Scholar 

  33. Fabiato A: Use of aequorin for the appraisal of the hypothesis of the release of calcium from the sarcoplasmic reticulum induced by a change of pH in skinned cardiac cells. Cell Calcium 6: 95–108, 1985

    Article  CAS  PubMed  Google Scholar 

  34. Lagadic-Gossmann D, Feuvray D: Intracellular sodium activity in papillary muscle from diabetic rat hearts. Exp Physiol 76: 147–149, 1991

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. United Arab Emirates University, Al Ain, UAE

    Frank Christopher Howarth & Anwar Qureshi

  2. University of Central Lancashire, Preston, Lancashire, UK

    Jaipaul Singh

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  1. Frank Christopher Howarth
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  2. Anwar Qureshi
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  3. Jaipaul Singh
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Howarth, F.C., Qureshi, A. & Singh, J. Effects of acidosis on ventricular myocyte shortening and intracellular Ca2+ in streptozotocin-induced diabetic rats. Mol Cell Biochem 261, 227–233 (2004). https://doi.org/10.1023/B:MCBI.0000028760.81889.98

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  • DOI: https://doi.org/10.1023/B:MCBI.0000028760.81889.98

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