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Various stressors rapidly activate the p38-MAPK signaling pathway in Mytilus galloprovincialis (Lam.)

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Abstract

The stimulation of p38-MAPK signal transduction pathway by various stressful stimuli was investigated in the marine bivalve M. galloprovincialis. Oxidative stress (5 μM H2O2) induced a biphasic pattern of p38-MAPK phosphorylation with maximal values attained at 15 min (8.1-fold) and 1 h (8.0-fold) of treatment respectively. Furthermore, 1 μM SB203580 abolished the p38-MAPK phosphorylation induced by oxidative stress. Aerial exposure also induced a biphasic pattern of p38-MAPK phosphorylation, with maximal values attained at 1 h (6.8-fold) and 8 h (4.9-fold) respectively. Re-oxygenation following a 15 min of aerial exposure resulted in the progressive dephosphorylation of the kinase. Treatment with 0.5 M sorbitol (in normal seawater) induced the rapid kinase phosphorylation (9.2-fold) and this effect was reversible. Seawater salinities varying between 100–60% had no effect, whereas a salinity of 50% induced a significant p38-MAPK phosphorylation. Furthermore, hypertonicity (120% seawater) resulted in a moderate kinase phosphorylation. All the above results demonstrate for the first time in a marine invertebrate imposed to environmental and other forms of stress as an intact, living organism, that the p38-MAPK pathway is specifically activated by various stressful stimuli which this animal can often face and sustain in vivo.

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Authors and Affiliations

  1. Department of Animal and Human Physiology, School of Biology, Faculty of Sciences, University of Athens, Panepistimioupolis, Athens, 157 84, Greece

    Catherine Gaitanaki, Erene Kefaloyianni, Athina Marmari & Isidoros Beis

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  1. Catherine Gaitanaki
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  2. Erene Kefaloyianni
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  3. Athina Marmari
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  4. Isidoros Beis
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Gaitanaki, C., Kefaloyianni, E., Marmari, A. et al. Various stressors rapidly activate the p38-MAPK signaling pathway in Mytilus galloprovincialis (Lam.). Mol Cell Biochem 260, 119–127 (2004). https://doi.org/10.1023/B:MCBI.0000026064.73671.91

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  • DOI: https://doi.org/10.1023/B:MCBI.0000026064.73671.91

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