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NF-κB prevents cells from undergoing Cr(VI)-induced apoptosis

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Abstract

The transcription factor NF-κB has been reported to prevent cells from undergoing apoptosis as well as promote cell apoptosis. To investigate the role of NF-κB in Cr(VI)-induced apoptosis, two cell lines were developed from human bronchial epithelial BEAS-2B cells: IKK cells, which were stably transfected with Iκ-Bα expression vector, that have normal NF-κB activity, and KM cells, which were stably transfected with mutated Iκ-Bα kinase expression vector, that exhibit very little NF-κB activity. With Cr(VI) stimulation, KM cells, but not IKK cells, exhibited substantial cell death. Cell morphological and TUNEL analyses indicated that the KM cells showed apoptotic features. These results suggest that NF-κB activation is required to prevent the cells from undergoing Cr(VI)-induced apoptosis.

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Authors and Affiliations

  1. Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV

    Suwei Wang, Fei Chen, Zhuo Zhang & Xianglin Shi

  2. Department of Basic Pharmaceutical Sciences, West Virginia University, Morgantown, WV, USA

    Suwei Wang, Zhuo Zhang & Xianglin Shi

  3. Mary Babb Randolph Cancer Center, Department of Microbiology, Immunology and Cell Biology, West Virginia University, Morgantown, WV, USA

    Bing-hua Jiang & Luo Jia

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  1. Suwei Wang
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Wang, S., Chen, F., Zhang, Z. et al. NF-κB prevents cells from undergoing Cr(VI)-induced apoptosis. Mol Cell Biochem 255, 129–137 (2004). https://doi.org/10.1023/B:MCBI.0000007269.74532.98

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