Abstract
Glutaryl-7-amino cephalosporanic acid acylase is a member of the N-terminal nucleophilic hydrolase family of enzymes. The crystal structure of the acylase reveals there is a Ser-His-Glu motif composed of Ser1β, His23β, and Glu455βnear the active site. This mimics the catalytic triad of Ser-His-Asp in serine proteases. Experiments prove that maturation of this enzyme involves autoproteolysis. It has been shown that Ser1βis the catalytic residue for the autoproteolysis and catalytic reaction. Our works on site-directed mutagenesis followed by the characterization of mutant enzymes demonstrated that His23βis essential for autoproteolysis whereas Glu455βis responsible for the efficiency of the process. Neither His23βnor Glu455βis essential for the acylase activity, although they affect the catalytic efficiency.
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Mao, X., Wang, W., Jiang, W. et al. His23β and Glu455β of the Pseudomonas sp. 130 Glutaryl-7-Amino Cephalosporanic Acid Acylase Are Crucially Important for Efficient Autoproteolysis and Enzymatic Catalysis. J Protein Chem 23, 197–204 (2004). https://doi.org/10.1023/B:JOPC.0000026415.96041.27
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DOI: https://doi.org/10.1023/B:JOPC.0000026415.96041.27