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Inactivation of Human Liver Arginase by Woodward's Reagent K: Evidence for Reaction with His141

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Abstract

Human liver arginase (EC 3.5.3.1) was totally inactivated by incubation with Woodward's reagent K (WRK). The inactivation followed pseudo–first-order kinetics, and the order of the inactivation was close to 1, consistent with reaction of one molecule of WRK with one subunit molecule for inactivation. The effect was totally reversed by 0.5 M hydroxylamine, and reactivated species were inactivated again by a second incubation with WRK. The pH dependence of the pseudo–first-order rate constants of inactivation indicated the participation of a ionizable residue with a pK a of 6.3 at 25°C. Replacement of His141 with phenylalanine rendered the enzyme totally resistant to the inactivation. We conclude that His141 is the residue whose chemical modification with WRK inactivates the enzyme.

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Authors
  1. Nelson Carvajal
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  2. Elena Uribe
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  3. Vasthi López
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  4. Mónica Salas
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Carvajal, N., Uribe, E., López, V. et al. Inactivation of Human Liver Arginase by Woodward's Reagent K: Evidence for Reaction with His141. J Protein Chem 23, 179–183 (2004). https://doi.org/10.1023/B:JOPC.0000026413.68088.e0

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  • DOI: https://doi.org/10.1023/B:JOPC.0000026413.68088.e0

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