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Global Analysis of Steady-State Energy Transfer Measurements in Membranes: Resolution of Structural and Binding Parameters

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Abstract

A method has been developed allowing structural and binding parameters to be recovered by global analysis of two-dimensional array of steady-state RET data in the special case where energy acceptors distribute between aqueous and lipid phases while donors are embedded in the membrane at a known depth. To test the validity of this approach, correlation and error analyses have been performed using simulated data. To exemplify the method application to the membrane studies, energy transfer from anthrylvinyl-labeled phosphatidylcholine incorporated into mixed phosphatidylcholine/cardiolipin unilamellar vesicles to heme group of cytochrome c is analyzed.

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REFERENCES

  1. J. Szälläsi, S. Damjanovich, S. A. Mulhern, and L. Troón (1987). Fluorescence energy transfer and membrane potential measurements monitoring dynamic properties of cell membranes: A critical review. Prog. Biophys. Mol. Biol. 49, 65-87.

    PubMed  Google Scholar 

  2. P. R. Selvin (1995). Fluorescence resonance energy transfer. Meth. Enzymol. 246, 300-334.

    PubMed  Google Scholar 

  3. J. Matko, M. Edidin (1997). Energy transfer methods in detecting molecular clusters on cell surfaces. Meth. Enzymol. 278, 444-462.

    PubMed  Google Scholar 

  4. S. Pedersen, K. Jærgensen, T. R. Bkmark, and O. G. Mouritsen (1996). Indirect evidence for lipid-domain formation in the transition region of phospholipid bilayers by two-probe fluorescence energy transfer. Biophys. J. 71, 554-560.

    PubMed  Google Scholar 

  5. L. M. S. Loura, A. Fedorov, and M. Prieto (2001). Fluid-fluid membrane microheterogeneity: A fluorescence resonance energy transfer study. Biophys. J. 80, 776-788.

    PubMed  Google Scholar 

  6. M. Subramanian, A. Jutila, and P. K. Kinnunen (1998). Binding and dissociation of cytochrome c to and from membranes containing acidic phospholipids. Biochemistry 37, 1394-1402.

    PubMed  Google Scholar 

  7. S. D. Zakharov, M. Lindeberg, and W. A. Cramer (1999). Kinetic description of structural changes linked to membrane import of the colicin E1 channel protein. Biochemistry 38, 11325-11332.

    PubMed  Google Scholar 

  8. J. B. Heymann, S. D. Zakharov, Y.-L. Zhang, and W. A. Cramer (1996). Characterization of electrostatic and nonelectrostatic components of protein-membrane binding interactions. Biochemistry 35, 2717-2725.

    PubMed  Google Scholar 

  9. T. J. T. Pinheiro, G. A. Ö, A. Watts, and H. Roder (1997). Structural and kinetic description of cytochrome c unfolding induced by the interaction with lipid vesicles. Biochemistry 36, 13122-13132.

    PubMed  Google Scholar 

  10. J. Eisinger, J. Flores, and R. M. Bookchin (1984). The cytosol-membrane interface of normal and sickle erythrocytes. Effect of hemoglobin deoxygenation and sickling. J. Biol. Chem. 259, 7169-7177.

    PubMed  Google Scholar 

  11. P. Wolber, B. Hudson (1979). An analytic solution to the FÖrster energy transfer problem in two dimensions. Biophys. J. 28, 197-210.

    PubMed  Google Scholar 

  12. G. P. Gorbenko and Ye. A. Domanov (2002). Energy transfer method in membrane studies: Some theoretical and practical aspects. J. Biochem. Biophys. Methods 52, 45-58.

    PubMed  Google Scholar 

  13. Ye. A. Domanov and G. P. Gorbenko (2002). Analysis of resonance energy transfer in model membranes: Role of orientational effects. Biophys. Chem. 99, 143-154.

    PubMed  Google Scholar 

  14. M. L. Johnson (1983). Evaluation and propagation of confidence intervals in nonlinear, asymmetrical variance spaces. Biophys. J. 44, 101-106.

    PubMed  Google Scholar 

  15. M. L. Johnson (2000). Parameter correlations while curve fitting. Meth. Enzymol. 321, 424-446.

    PubMed  Google Scholar 

  16. J. R. Lakowicz (1999). Principles of Fluorescence Spectroscopy, 2nd ed., Kluwer Academic, New York.

    Google Scholar 

  17. J. M. Beechem and E. Haas (1989). Simultaneous determination of intramolecular distance distribution and conformational dynamics by global analysis of energy transfer measurements. Biophys. J. 55, 1225-1236.

    PubMed  Google Scholar 

  18. J. M. Beechem (1992). Global analysis of biochemical and biophysical data. Meth. Enzymol. 210, 37-54.

    PubMed  Google Scholar 

  19. Yu. V. Kholin, D. S. Konyaev, and S. A. Mernyi (1999). Construction of complexation models: From measurements to final verdict. Kharkov Uni. Bull. No. 437, Chem. Ser. 26, 17-35.

    Google Scholar 

  20. L. Zekany and I. Nagypal (1985). Computational Methods for the Determination of Formation Constants, Plenum, New York.

    Google Scholar 

  21. J. G. Molotkovsky, P. I. Dmitriev, L. F. Nikulina, and L. D. Bergelson (1979). Synthesis of new fluorescently labeled phosphatidylcholines. Bioorg. Khim. 5, 588-594.

    Google Scholar 

  22. Z. Gryczynsky, J. Lubkowski, and E. Bucci (1995). Heme-protein interactions in horse heart myoglobin at neutral pH and exposed to acid investigated by time-resolved fluorescence in the pico-to nanosecond time range. J. Biol. Chem. 270, 19232-19237.

    PubMed  Google Scholar 

  23. L. M. S. Loura, A. Fedorov, and M. Prieto (1996). Resonance energy transfer in a model system of membranes: Application to gel and liquid crystalline phases. Biophys. J. 71, 1823-1836.

    PubMed  Google Scholar 

  24. Q. Chen and B. R. Lentz (1997). Fluorescence resonance energy transfer study of shape changes in membrane-bound bovine prothrombin and meizothrombin. Biochemistry 36, 4701-4711.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Biological and Medical Physics, V. N. Karazin Kharkiv National University, Kharkiv, Ukraine

    Yegor A. Domanov & Galina P. Gorbenko

  2. Laboratory of Lipid Chemistry, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia

    Julian G. Molotkovsky

Authors
  1. Yegor A. Domanov
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  2. Galina P. Gorbenko
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  3. Julian G. Molotkovsky
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Correspondence to Yegor A. Domanov.

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Domanov, Y.A., Gorbenko, G.P. & Molotkovsky, J.G. Global Analysis of Steady-State Energy Transfer Measurements in Membranes: Resolution of Structural and Binding Parameters. Journal of Fluorescence 14, 49–55 (2004). https://doi.org/10.1023/B:JOFL.0000014659.14875.47

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  • DOI: https://doi.org/10.1023/B:JOFL.0000014659.14875.47

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