Abstract
Dendrimers are globular, hyperbranched polymers which possess a high concentration of surface functional groups and internal cavities. These unique features make them very useful to many biomedical applications, especially as carrier molecules. This study presents results of estimation of polyamidoamine (PAMAM) dendrimers and human serum albumin (HSA) binding capacity of fluorescent probe 1-anilinonaphthalene-8-sulfonic acid (ANS). It has been shown that fluorescent probes can be used for quantitative analysis of dendrimers' binding capacity.
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D. A. Tomalia, A. M. Naylor, and W. A. Goddard III (1990). Starburst dendrimers: Molecular-level control of size, shape, surface chemistry, topology, and flexibility from atoms to macroscopic matter. Angew. Chem. Int. Ed. 29, 138-175.
M. Fisher and F. Vögtle (1999). Dendrimers: From design to application—A progress report. Angew. Chem. Int. Ed. 38, 884-905.
M. J. Cloninger (2002). Biological applications of dendrimers. Curr. Opin. Chem. Biol. 6, 742-748.
D. Zanini and R. Roy (1998). Practical synthesis of Starburst PAMAM α-thiosialodendrimers for probing multivalent carbohydrate-lectin binding properties. J. Org. Chem. 63, 3486-3491.
C. Kojima, K. Kono, K. Maruyama, and T. Takagishi (2000). Synthesis of polyamidoamine dendrimers having poly(ethylene glycol) grafts and their ability to encapsulate anticancer drugs. Bioconjugate Chem. 11, 910-917.
V. B. Gavrilov (2002). in I. D. Volotovski (Ed.), Biophysics of Living Systems: From Molecule to Organism, BelSens, Minsk, Belarus, pp. 134-147.
M. S. Highley and E. A. De Bruijn (1996). Erythrocytes and the transport of drugs and endogenous compounds. Pharm. Res. 13, 186-195.
U. Kragh-Hansen (1990). sStructure and ligand binding properties of human serum albumin. Med. Bull. 37,57-84.
Yu. A. Vladimirov and G. E. Dobretsov (1980). Fluorescent Probes in Studying Biological Membranes, Nauka, Moscow, Russia.
G. E. Dobretsov (1994). in Yu. A. Grizunov and G. E. Dobretsov (Eds.), Blood Serum Albumin in Clinical Medicine, Irius, Moscow, Russia.
M. A. Kiselev, Yu. A. Grizunov, G. E. Dobretsov, and M. N. Komarov (2001). The size of human serum albumin molecules in solution. Biophysics 46, 423-427.
G. Sudlow, D. J. Birkett, and D. N. Wade (1975). The characterization of two specific drug binding sites on human serum albumin. Mol. Pharmacol. 11, 824-832.
M. A. Landau (1985). Molecular Nature of Some Physiological Processes, Nauka, Moscow, Russia.
G. Scatchard (1949). The attractions of proteins for small molecules and ions. Ann. N. Y. Acad. Sci. 51, 660-672.
J. Slavik (1982). Anilinonaphthalene sulfonate as a probe of membrane composition and function. Biochim. Biophys. Acta 694,1-25.
B. Klajnert, L. Stanislawska, M. Bryszewska, and B. Palecz (2003). Interactions between PAMAM dendrimers and bovine serum albumin. Biochim. Biophys. Acta 1648, 115-126.
B. Klajnert and M. Bryszewska (2002). The interaction of tryptophan and ANS with PAMAM dendrimers. Cell. Mol. Biol. Lett. 7, 1087-1094.
A. M. Naylor and W. A. Goddard III (1989). Starburst dendrimers. 5. Molecular shape control. J. Am. Chem. Soc. 111, 2339-2341.
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Shcharbin, D., Klajnert, B., Mazhul, V. et al. Estimation of PAMAM Dendrimers' Binding Capacity by Fluorescent Probe ANS. Journal of Fluorescence 13, 519–524 (2003). https://doi.org/10.1023/B:JOFL.0000008063.28420.45
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DOI: https://doi.org/10.1023/B:JOFL.0000008063.28420.45