Skip to main content
SpringerLink
Log in

Differential Participation of Central L-Glutamate Receptors of non-NMDA-Subtype in Associative Learning of the Honeybee Apis mellifera

  • Published:
Journal of Evolutionary Biochemistry and Physiology Aims and scope Submit manuscript

Abstract

Participation of non-NMDA-receptors in associative learning in the honeybee was analyzed using a complex approach. There were studied effects of systemic and intracerebral AMPA injections on retention of the elaborated conditional reflex in short-term memory, on dynamics of the bioelectrical activity of neurons of calyx of the mushroom bodies, and on the limb locomotor activity. It has been shown that the systemic and intracerebral AMPA injections stimulate processes of short-term memory and activate bioelectrical activity of neurons of the mushroom body medial calyx. Antagonist of AMPA-receptors NS-257 HCl suppressed spontaneous activity of these neurons. The observed processes did not depend on the character of locomotor activity. These results confirm the hypothesis proposed earlier about involvement of non-NMDA-receptors in the process of associative learning.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. Lopatina, N.G., Ryzhova, I.V., Chesnokova, E.G., and Dmitrieva, L.A., L-Glutamate Receptors in the Central Nervous System of the Honeybee Apis mellifera and their Role in the Process of Elaboration of Conditional Reflex and of Memory Traces, Zh. Evol. Biokhim. Fiziol., 1997, vol. 33, pp. 506–514.

    Google Scholar 

  2. Lopatina, N.G., Ryzhova, I.V., Chesnokova, E.G., The Role of non-NMDA-Receptors in the Process of Associative Learning in the Honeybee Apis mellifera, Zh. Evol. Biokhim. Fiziol., 2002, vol. 38, pp. 163–168.

    Google Scholar 

  3. Lopatina, N.G., Ryzhova, I.V., Chesnokova, E.G., and Dmitrieva, L.A., N-Methyl-D-Aspartate Receptors in Formation of the Short-Term Memory of the Honeybee Apis mellifera, Zh. Evol. Biokhim. Fiziol., 2000, vol. 36, pp. 223–228.

    Google Scholar 

  4. Kucharski, R., Ball, E., Hayward, D., and Maleszka, R., Molecular Cloning and Expression Analysis of a cDNA Encoding a Glutamate Transporter in the Honeybee Brain, Gene, 2000, vol. 242, pp. 399–405.

    Google Scholar 

  5. Neuhaus, B. and Muller, U., Involvement of Glutamate Metabolism in Honeybee Olfactory Learning, Proceedings of the 2001 Berlin Meeting of the European Section of IUSSI, 2001, p. 222.

  6. Osborn, R.H., Insect Neurotransmission: Neurotransmitters and their Receptors, Pharmacol. Ther., 1996, vol. 69, pp. 117–142.

    Google Scholar 

  7. Menzel, R., Memory Dynamics in the Honeybees, J. Comp. Physiol., 1999, vol. 185, pp. 323–340.

    Google Scholar 

  8. 8._ Menzel, R., Heyne, A., Kinzel, C., Gerber, B., and Fiala, A., Pharmacological Dissociation between the Brain Forcing Sensitizing, and Response-Releasing Function of Reward in Honeybee Classical Conditioning, Behav. Neurosci., 1999, vol. 113, pp. 744–754.

    Google Scholar 

  9. Menzel, R., Durst, C., and Erber, J., The Mushroom Bodies in the Honeybee: From Molecules to Behavior, Fortschr. Zool., 1994, vol. 39, pp. 82–102.

    Google Scholar 

  10. Magazanik, L.G., Bolshakov, K.V., Buldakova, S.L., Gmiro, V.E., Dorofeeva, N.A., Lukomskaya, N.Ya., Potalieva, N.N., Samoilova, M.V., Tikhonov, D.B., and Fedorova, E.V., Structural Peculiarities of the Ionotropic Glutamate Receptors Detected by Channel Blockade, Ross. Fiziol. Zh. RAN, 2000, vol. 86, pp. 1138–1151.

    Google Scholar 

  11. Porter, R.H.P. and Greenmyre, J.T., Regional Variations in the Pharmacology of AMPA Receptors as Revealed by Receptor Autoradiography, Brain Res., 1994, vol. 664, pp. 202–206.

    Google Scholar 

  12. Magazanik, L.G., Ion Channel Blockade as an Approach to the Study of AMPA-Receptor Subtype, Ross. Fiziol. Zh. RAN, 1988, vol. 84, pp. 994–1005.

    Google Scholar 

  13. 13._ Betz, H., Schuster, Ch., Ultsch, A., and Schmitt, B., Molecular Biology of Ionotropic Glutamate Receptors in Drosophila melanogaster, TiPS, 1993, vol. 14, pp. 428–430.

    Google Scholar 

  14. Antonio, A.Di, Peterson, S.A., Heckmann, M., and Goodman, C.S., Glutamate Receptors Expression Regulates Quantal Size and Quantal Content at the Drosophila Neuromuscular Junction, J. Neurosci., 1999, vol. 19, pp. 3023–3032.

    Google Scholar 

  15. 15._ Bicker, G., Histochemistry of Classical Neurotransmitters in Antennal Lobes and Mushroom Bodies of the Honeybee, Microsc. Res. Tech., 1999, vol. 45, pp. 174–183.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, Russia

    N. N. Lopatina, T. G. Zachepilo, I. V. Ryzhova, V. B. Smirnov & E. G. Chesnokova

Authors
  1. N. N. Lopatina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. G. Zachepilo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. I. V. Ryzhova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. V. B. Smirnov
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. E. G. Chesnokova
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lopatina, N.N., Zachepilo, T.G., Ryzhova, I.V. et al. Differential Participation of Central L-Glutamate Receptors of non-NMDA-Subtype in Associative Learning of the Honeybee Apis mellifera . Journal of Evolutionary Biochemistry and Physiology 40, 271–276 (2004). https://doi.org/10.1023/B:JOEY.0000042630.34824.ae

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/B:JOEY.0000042630.34824.ae

Keywords

Search

Navigation