Journal of Chemical Ecology

, Volume 23, Issue 3, pp 759–777 | Cite as

Tall Larkspur Ingestion: Can Cattle Regulate Intake Below Toxic Levels?

  • James A. Pfister
  • Frederick D. Provenza
  • Gary D. Manners
  • Dale R. Gardner
  • Michael H. Ralphs
Article

Abstract

Tall larkspur (Delphinium barbeyi) is a toxic forb often consumed by cattle on mountain rangelands, with annual fatalities averaging about 5%. This study examined the relationship between food ingestion and toxicity in cattle. Two grazing studies suggested that larkspur consumption above 25–30% of cattle diets for one or two days led to reduced larkspur consumption on subsequent days. We subsequently hypothesized that cattle can generally limit intake of larkspur to sublethal levels. This hypothesis was tested by feeding a 27% larkspur pellet in experiment 1. Cattle given a 27% larkspur pellet ad libitum showed distinct cyclic patterns of intake, where increased larkspur consumption on one or two days was followed by reduced (P < 0.025) consumption on the following day. The amount of larkspur (mean 2007 g/day; 17.8 mg toxic alkaloid/kg body wt) consumed was just below a level that would produce overt signs of toxicity. Experiment 2 was conducted to examine cattle response to a toxin dose that varied with food intake. Lithium chloride (LiCl) paired with corn ingestion was used as a model toxin, and we hypothesized that if increased (decreased) consumption was followed by a stronger (weaker) dose of LiCl, cattle would show a transient reduction (increase) in corn intake. There was no difference (P > 0.05) between controls and treatment animals at the 20 or 40 mg LiCl/kg dose in the percentage of corn consumed, but the 80 mg LiCl/kg dose induced a cyclic response (mean 46%) compared to intake by controls (mean 96%) (P < 0.001). At the 80 mg/kg dose, LiCl induced an aversion to corn; when corn intake decreased on subsequent days and LiCl dose also decreased, cattle responded by increasing corn intake and apparently extinguishing the transient food aversion. Experiment 3 was similar to the LiCl trial, except that tall larkspur was the toxin. Cattle responded to oral gavage of ground larkspur with distinct cycles; days of higher corn consumption were followed by one to three days of reduced consumption. Corn intake for controls was higher (P < 0.01) than for larkspur-treated animals (means 84 and 52%, respectively; day × treatment interaction P < 0.01). The threshold for toxic effects on corn intake was 14 mg toxic alkaloid/kg body weight. In conclusion, cattle apparently limit ingestion of some toxins so that periods of high consumption are followed by periods of reduced consumption to allow for detoxification. Cyclic consumption generally enables cattle to regulate tall larkspur consumption below a toxic threshold and allows cattle the opportunity to safely use an otherwise nutritious, but toxic, plant.

Larkspur alkaloids intake regulation toxicity cattle 

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REFERENCES

  1. BENN, M. H., and JACYNO, J. 1983. The toxicology and pharmacology of diterpenoid alkaloids, pp. 153–210, in S. W. Pelletier (ed.). Alkaloids: Chemical and Biological Perspectives. John Wiley & Sons, New York.Google Scholar
  2. BURRITT, E. A., and PROVENZA, F. D. 1989. Food aversion learning: Ability of lambs to distinguish safe from harmful foods. J. Anim. Sci. 70:1732–1739.Google Scholar
  3. BURRITT, E. A., and PROVENZA, F. D. 1991. Ability of lambs to learn with a delay between food ingestion and consequences given meals containing novel and familiar foods. Appl. Anim. Behav. Sci. 32:179–189.Google Scholar
  4. BURRITT, E. A., and PROVENZA, F. D. 1996. Amount of experience and prior illness affect the acquisition and persistence of conditioned food aversions in lambs. Appl. Anim. Behav. Sci. 48:73–80.Google Scholar
  5. DOBELIS, P., MADL, J. E., MANNERS, G. D., PFISTER, J. A., and WALROND, J. P. 1993. Antagonism of nicotinic receptors by Delphinium alkaloids. Neurosci. Abstr. 631:12.Google Scholar
  6. DUTOIT, J. T., PROVENZA, F. D., and NASTIS, A. 1991. Conditioned food aversions: How sick must a ruminant get before it learns about toxicity in foods? Appl. Anim. Behav. Sci. 30:35–46.Google Scholar
  7. FREELAND, W. J., and JANZEN, D. H. 1974. Strategies in herbivory by mammals: the role of plant secondary compounds. Am. Nat. 108:269.Google Scholar
  8. FREELAND, W. J., and SALADIN, L. R. 1989. Choice of mixed diets by herbivores: The idiosyncratic effects of plant secondary compounds. Biochemical Syst. Ecol. 17:493–497.Google Scholar
  9. KYRIAZAKIS, I., and EMMANS, G. C. 1992. Selection of a diet by growing pigs given choices between foods differing in contents of protein and rapeseed meal. Appetite 19:121–132.PubMedGoogle Scholar
  10. KYRIAZAKIS, I., and OLDHAM, J. D. 1993. Diet selection in sheep: The ability of growing lambs to select a diet that meets their crude protein (nitrogen × 6.25) requirements. Br. J. Nutr. 69:617–629.PubMedGoogle Scholar
  11. LANE, M. A., RALPHS, M. H., OLSEN, J. D., PROVENZA, F. D., and PFISTER, J. A. 1990. Conditioned taste aversion: potential for reducing cattle loss to tall larkspur. J. Range Manage. 43:127–131.Google Scholar
  12. LAUNCHBAUGH, K. L., PROVENZA, F. D., and BURRITT, E. A. 1993. How herbivores track variable environments: Response to variability of phytotoxins. J. Chem. Ecol. 19:1047–1056.Google Scholar
  13. LEFKOWITZ, R. J., HOFMAN, B. B., and TAYLOR, P. 1990. Neurohumoral transmission: The autonomic and somatic motor systems, pp. 84–121, in A. G. Gilman, T. W. Rall, A. S. Nies, and P. Taylor (eds.). The Pharmacological Basis of Therapeutics, 8th ed. Pergamon Press, New York.Google Scholar
  14. MANNERS, G. D., and PFISTER, J. A. 1993. Normal phase liquid chromatographic analysis of toxic norditerpenoid alkaloids. Phytochem. Anal. 4:14–18.Google Scholar
  15. MANNERS, G. D., PFISTER, J. A., RALPHS, M. H., OLSEN, J. D., and PANTER, K. E. 1992. Larkspur chemistry: Toxic alkaloids in tall larkspurs. J. Range Manage. 45:63–67.Google Scholar
  16. MANNERS, G. D., PANTER, K. E., RALPHS, M. H., PFISTER, J. A., and OLSEN, J. D. 1993. The occurrence and toxic evaluation of norditerpenoid alkaloids in the tall larkspurs (Delphinium sp.). J. Food Agric. Chem. 41:96–100.Google Scholar
  17. MANNERS, G. D., PANTER, K. E., and PELLETIER, S. W. 1995. Structure-activity relationships of norditerpenoid alkaloids occurring in toxic larkspur (Delphinium) species. J. Nat. Prod. 58:863–869.PubMedGoogle Scholar
  18. NEWMAN, J. A., PARSONS, A. J., and HARVEY, A. 1992. Not all sheep prefer clover: Diet selection revisited. J. Agric. Sci. Camb. 119:275–283.Google Scholar
  19. OLSEN, J. D., and RALPHS, M. H. 1986. Feed aversion induced by intraruminal infusion with larkspur extract in cattle. Am. J. Vet. Res. 47:1829–1833.PubMedGoogle Scholar
  20. PFISTER, J. A., and MANNERS, G. D. 1991. Mineral supplementation of cattle grazing larkspur-infested rangeland during drought. J. Range Manage. 44:105–111.Google Scholar
  21. PFISTER, J. A., and CHENEY, C. D. 1997. Operant behavioral analysis of acute tall larkspur (Delphinium barbeyi) intoxication in cattle. Behaviorology. In press.Google Scholar
  22. PFISTER, J. A., MANNERS, G. D., RALPHS, M. H., HONG, Z. X., and LANE, M. A. 1988a. Effects of phenology, site and rumen fill on tall larkspur consumption by cattle. J. Range Manage. 41:509–514.Google Scholar
  23. PFISTER, J. A., RALPHS, M. H., and MANNERS, G. D. 1988b. Cattle grazing tall larkspur on Utah mountain rangeland. J. Range Manage. 41:118–122.Google Scholar
  24. PFISTER, J. A., ADAMS, D. C., ARAMBEL, M. J., OLSEN, J. D., and JAMES, L. F. 1989. Sublethal levels of toxic larkspur: effects on intake and rumen dynamics in cattle. Nutr. Rep. Int. 40:629–636.Google Scholar
  25. PFISTER, J. A., PROVENZA, F. D., and MANNERS, G. D. 1990. Ingestion of tall larkspur by cattle: Separating effects of flavor from postingestive consequences. J. Chem. Ecol. 16:1697–1705.Google Scholar
  26. PFISTER, J. A., MANNERS, G. D., GARDNER, D. R., and RALPHS, M. H. 1994a. Toxic alkaloid levels in tall larkspur (Delphinium barbeyi) in western Colorado. J. Range Manage. 47:355–358.Google Scholar
  27. PFISTER, J. A., PANTER, K. E., and MANNERS, G. D. 1994b. Effective dose in cattle of toxic alkaloids from tall larkspur (Delphinium barbeyi). Vet. Hum. Toxicol. 36:10–11.PubMedGoogle Scholar
  28. PFISTER, J. A., PANTER, K. E., MANNERS, G. D., and CHENEY, C. D. 1994c. Reversal of tall larkspur (Delphinium barbeyi) toxicity with physostigmine. Vet. Hum. Toxiciol. 36:511–514.Google Scholar
  29. PROVENZA, F. D. 1995. Postingestive feedback as an elementary determinant of food selection and intake in ruminants. J. Range Manage. 48:2–17.Google Scholar
  30. PROVENZA, F. D. 1996. Acquired aversions as the basis for varied diets of foraging on rangelands. J. Anim. Sci. 74:2010–2020.PubMedGoogle Scholar
  31. PROVENZA, F. D., PFISTER, J. A., and CHENEY, C. D. 1992. Mechanisms of learning in diet selection with reference to phytotoxicosis in herbivores. J. Range Manage. 45:36–45.Google Scholar
  32. PROVENZA, F. D., NOLAN, J. V., and LYNCH, J. J. 1993. Temporal contiguity between food ingestion and toxicosis affects the acquisition of food aversions in sheep. Appl. Anim. Behav. Sci. 38:269–281.Google Scholar
  33. PROVENZA, F. D., LYNCH, J. J., BURRITT, E. A., and SCOTT, C. B. 1994. How goats learn to distinguish between novel foods that differ in postingestive consequences. J. Chem. Ecol. 20:609–624.Google Scholar
  34. RALPHS, M. H. 1992. Conditioned food aversion: training livestock to avoid eating poisonous plants. J. Range Manage. 45:46–51.Google Scholar
  35. RALPHS, M. H., and CHENEY, C. D. 1993. Influence of cattle age, lithium chloride dose level, and food type in retention of food aversions. J. Anim. Sci. 71:373–379.PubMedGoogle Scholar
  36. RALPHS, M. H., and OLSEN, J. D. 1990. Adverse influence of social facilitation and learning context in training cattle to avoid eating larkspur. J. Anim. Sci. 68:1944–1952.PubMedGoogle Scholar
  37. RALPHS, M. H., OLSEN, J. D., PFISTER, J. A., and MANNERS, G. D. 1988. Plant-animal interactions in larkspur poisoning in cattle. J. Anim. Sci. 66:2334–2342.PubMedGoogle Scholar
  38. RILEY, A. L., and TUCK, D. L. 1985. Conditioned taste aversions: A behavioral index of toxicity, pp. 272–292, in N. S. Braveman and P. Bronstein (eds.). Experimental Assessments and Clinical Applications of Conditioned Food Aversions. New York Academy of Science, New York.Google Scholar
  39. SAS. 1987. SAS/STAT User's Guide, Version 6 Edition, SAS Institute Inc., Cary, North Carolina.Google Scholar
  40. TAYLOR, P. 1990. Agents acting at the neuromuscular junction and autonomic ganglia, pp. 166–186. in A. G. Gilman, T. W. Rall, A. S. Nies, and P. Taylor (eds.). The Pharmacological Basis of Therapeutics, 8th ed. Pergamon Press, New York.Google Scholar
  41. VILLALBA, J. J., and PROVENZA, F. D. 1997. Preference for wheat straw by lambs conditioned with intraruminal infusions of starch. Br. J. Nutr. In press.Google Scholar
  42. WALLWORK, J. C., FOSMIRE, G. J., and SANDSTEAD, H. H. 1981. Effect of zinc deficiency on appetite and plasma amino acid concentrations in the rat. Br. J. Nutr. 45:127–136.PubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1997

Authors and Affiliations

  • James A. Pfister
  • Frederick D. Provenza
  • Gary D. Manners
  • Dale R. Gardner
  • Michael H. Ralphs

There are no affiliations available

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