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Griscelli Syndrome: Characterization of a New Mutation and Rescue of T-Cytotoxic Activity by Retroviral Transfer of RAB27A Gene

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Abstract

Griscelli syndrome (GS) is caused by mutations in the MYO5A (GS1), RAB27A (GS2), or MLPH (GS3) genes, all of which lead to a similar pigmentary dilution. In addition, GS1 patients show primary neurological impairment, whereas GS2 patients present immunodeficiency and periods of lymphocyte proliferation and activation, leading to their infiltration in many organs, such as the nervous system, causing secondary neurological damage. We report the diagnosis of GS2 in a 4-year-old child with haemophagocytic syndrome, immunodeficiency, and secondary neurological disorders. Typical melanosome accumulation was found in skin melanocytes and pigment clumps were observed in hair shafts. Two heterozygous mutant alleles of the RAB27A gene were found, a C-T transition (C352T) that leads to Q118stop and a G-C transversion on the exon 5 splicing donor site (G467+1C). Functional assays showed increased cellular activation and decreased cytotoxic activity of NK and CD8+ T cells, associated with defective lytic granules release. Myosin-Va expression and localization in the patient lymphocytes were also analyzed. Most importantly, we show that cytotoxic activity of the patient's CD8+ T lymphocytes can be rescued in vitro by RAB27A gene transfer mediated by a recombinant retroviral vector, a first step towards a potential treatment of the acute phase of GS2 by RAB27A transduced lymphocytes.

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Authors and Affiliations

  1. Departamentos de Biologia Celular, Molecular e Bioagentes Patogênicos, São Paulo, Brazil

    João C. S. Bizario

  2. Departamentos de Curso de Medicina da Universidade de Ribeirão Preto (UNAERP), São Paulo, Brazil

    João C. S. Bizario

  3. Departamentos de Unité, de Recherches sur le Dévelopement Normal et Pathologique du Système Immunitaire, INSERM U-429, Paris, France

    Jérôme Feldmann, Gaël Ménasché & Geneviève de Saint-Basile

  4. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas da Faculdade de Ciências Farmacêuticas de Ribeirão Preto-USP, São Paulo, Brazil

    Fabíola A. Castro

  5. Departamentos de Clínica Médica da Faculdade de Medicina de Ribeirão, Preto-Universidade de São Paulo (USP), Brazil

    Fabíola A. Castro & Júlio C. Voltarelli

  6. Departamento de Pediatria da Faculdade de Medicina-USP, São Paulo, Brazil

    Cristina M. A. Jacob, L. Cristofani & Erasmo B. Casella

  7. Departamentos de Biologia Celular, Molecular e Bioagentes Patogênicos, São Paulo, Brazil

    Enilza M. Espreafico

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  1. João C. S. Bizario
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  2. Jérôme Feldmann
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  9. Geneviève de Saint-Basile
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Bizario, J.C.S., Feldmann, J., Castro, F.A. et al. Griscelli Syndrome: Characterization of a New Mutation and Rescue of T-Cytotoxic Activity by Retroviral Transfer of RAB27A Gene. J Clin Immunol 24, 397–410 (2004). https://doi.org/10.1023/B:JOCI.0000029119.83799.cb

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  • DOI: https://doi.org/10.1023/B:JOCI.0000029119.83799.cb

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