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Tapasin Decreases Immune Responsiveness to a Model Tumor Antigen

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Abstract

The T-cell response against cancer is dependent on the cell surface presentation of tumor-associated or tumor-specific peptides by major histocompatibility complex (MHC) class I molecules. We found that tapasin, a chaperone protein that normally assists in the assembly of MHC class I molecules, is undetectable in an unstimulated pancreatic tumor cell line, Panc02, and only very weakly expressed after γ-interferon stimulation. Transfection of tapasin into the Panc02 cells did not quantitatively increase MHC class I surface expression or detectably affect MHC class I association with peptide and β2-microglubulin (β2m). However, we found that transfected tapasin downregulated immune reactivity against a model tumor antigen, MUC1. Although tapasin has been previously shown by others to increase immune recognition of particular antigens, our results suggest that tapasin has a negative impact on the presentation of an immunodominant epitope from a specific model tumor antigen.

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Authors and Affiliations

  1. Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska

    Heth R. Turnquist, Karl G. Kohlgraf, Mary M. McIlhaney, Michael A. Hollingsworth & Joyce C. Solheim

  2. Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska

    Heth R. Turnquist, Karl G. Kohlgraf, R. Lee Mosley, Michael A. Hollingsworth & Joyce C. Solheim

  3. Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska

    Michael A. Hollingsworth & Joyce C. Solheim

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  1. Heth R. Turnquist
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  2. Karl G. Kohlgraf
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  3. Mary M. McIlhaney
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  4. R. Lee Mosley
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  5. Michael A. Hollingsworth
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  6. Joyce C. Solheim
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Turnquist, H.R., Kohlgraf, K.G., McIlhaney, M.M. et al. Tapasin Decreases Immune Responsiveness to a Model Tumor Antigen. J Clin Immunol 24, 462–470 (2004). https://doi.org/10.1023/B:JOCI.0000029118.51587.d9

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  • DOI: https://doi.org/10.1023/B:JOCI.0000029118.51587.d9

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