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Functional Characterization of Human Tc0, Tc1 and Tc2 CD8+ T Cell Clones: Control of X4 and R5 HIV Strain Replication

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Abstract

CD8+ T lymphocytes have the potential ability to inhibit human immunodeficiency virus (HIV) replication, by secreting soluble(s) factor(s) known as CD8+ T lymphocyte antiviral factor (CAF). A panel of CD8+ and CD4+ T cell clones from HIV1-infected and uninfected donors were generated to better define the phenotype of CAF-producing cells. We first verified that the different CD4+ T cell subsets (Th0, Th1, and Th2) were productively infected by X4 and R5 virus strains. X4 viral replication in CD4+ T cells was controlled by the three CD8+ T cell subsets (Tc0, Tc1, and Tc2); however, the frequency of Tc clones controlling R5 strain was much lower with a dramatic absence of this activity among Tc clones from uninfected donor. Finally, capacity to control viral replication showed an heterogeneity: some clones could control both virus strains, some controlled only the X4 virus, whereas the majority exerted no suppressive activity.

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Authors and Affiliations

  1. Unité d'Immunopathologie Virale, URA CNRS 1930, Institut Pasteur, 28 rue du Docteur Roux, 75724, Paris cedex 15, France

    Michèle Février, Sylvie le Borgne & Yves Rivière

  2. Service de la lutte anti-vénérienne, 97300, Cayenne, French Guiana

    Christian Marty

  3. Centre hospitalier Général de Cayenne, 97306, Cayenne, French Guiana

    Antoine Talarmin

Authors
  1. Michèle Février
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  2. Sylvie le Borgne
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  3. Christian Marty
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  4. Antoine Talarmin
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  5. Yves Rivière
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Correspondence to Michèle Février.

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Février, M., Borgne, S.l., Marty, C. et al. Functional Characterization of Human Tc0, Tc1 and Tc2 CD8+ T Cell Clones: Control of X4 and R5 HIV Strain Replication. J Clin Immunol 24, 272–280 (2004). https://doi.org/10.1023/B:JOCI.0000025448.08570.2d

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  • DOI: https://doi.org/10.1023/B:JOCI.0000025448.08570.2d

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