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Novel Techniques for Weak Alignment of Proteins in Solution Using Chemical Tags Coordinating Lanthanide Ions

Journal of Biomolecular NMR volume 29, pages 339–349 (2004)Cite this article

Abstract

A molecule with an anisotropic magnetic susceptibility is spontaneously aligned in a static magnetic field. Alignment of such a molecule yields residual dipolar couplings and pseudocontact shifts. Lanthanide ions have recently been successfully used to provide an anisotropic magnetic susceptibility in target molecules either by replacing a calcium ion with a lanthanide ion in calcium-binding proteins or by attaching an EDTA derivative to a cysteine residue via a disulfide bond. Here we describe a novel enantiomerically pure EDTA derived tag that aligns stronger due to its shorter linker and does not suffer from stereochemical diversity upon lanthanide complexation. We observed residual 15N,1H-dipolar couplings of up to 8 Hz at 800 MHz induced by a single alignment tensor from this tag.

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Affiliations

  1. Max-Planck Institute for Biophysical Chemistry, Department of NMR Based Structural Biology, University of Frankfurt, am Faßberg 11, D-37077, Göttingen, Germany

    Takahisa Ikegami, Laurent Verdier, Peyman Sakhaii & Christian Griesinger

  2. Institute for Organic Chemistry, Marie-Curie-Str. 11, D-60439, Frankfurt am Main, Germany

    Susanne Grimme, Barbara Pescatore & Krishna Saxena

  3. Affinium Pharmaceutical, 100 University Avenue, Toronto, Ontario, M5J 1V6, Canada

    Klaus M. Fiebig

Authors
  1. Takahisa Ikegami
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  2. Laurent Verdier
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  3. Peyman Sakhaii
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  4. Susanne Grimme
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  5. Barbara Pescatore
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  6. Krishna Saxena
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  7. Klaus M. Fiebig
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  8. Christian Griesinger
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Ikegami, T., Verdier, L., Sakhaii, P. et al. Novel Techniques for Weak Alignment of Proteins in Solution Using Chemical Tags Coordinating Lanthanide Ions. J Biomol NMR 29, 339–349 (2004). https://doi.org/10.1023/B:JNMR.0000032611.72827.de

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  • DOI: https://doi.org/10.1023/B:JNMR.0000032611.72827.de

  • dipolar coupling
  • disulfide bond
  • lanthanide ion
  • molecular alignment
  • NMR