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Triple Resonance MAS NMR with (13C, 15N) Labelled Molecules: Reduced Dimensionality Data Acquisition Via 13C-15N Heteronuclear Two-Spin Coherence Transfer Pathways

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Abstract

A reduced dimensionality magic angle spinning solid-state NMR experimental protocol for obtaining chemical shift correlation spectra of dipolar coupled nuclei in uniformly (13C, 15N) labelled biological systems is described and demonstrated. The method involves a mapping of the evolution frequencies of heteronuclear 13C-15N zero- and double-quantum coherences. In comparison to a reduced dimensionality procedure involving the simultaneous incrementation of two single-quantum chemical shift evolution periods, the approach described here could be potentially advantageous for minimising the heat dissipated in the probe by high power 1H decoupling in experiments requiring long t 1 acquisition times.

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Authors and Affiliations

  1. Abteilung Molekulare Biophysik/NMR-Spektroskopie, Institut für Molekulare Biotechnologie, 07745, Jena, Germany

    Jörg Leppert, Bert Heise, Oliver Ohlenschläger, Matthias Görlach & Ramadurai Ramachandran

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  1. Jörg Leppert
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  2. Bert Heise
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  3. Oliver Ohlenschläger
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  4. Matthias Görlach
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  5. Ramadurai Ramachandran
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Leppert, J., Heise, B., Ohlenschläger, O. et al. Triple Resonance MAS NMR with (13C, 15N) Labelled Molecules: Reduced Dimensionality Data Acquisition Via 13C-15N Heteronuclear Two-Spin Coherence Transfer Pathways. J Biomol NMR 28, 185–190 (2004). https://doi.org/10.1023/B:JNMR.0000013834.36262.79

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  • DOI: https://doi.org/10.1023/B:JNMR.0000013834.36262.79

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