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Phosphorus-31 Transverse Relaxation Rate Measurements by NMR Spectroscopy: Insight into Conformational Exchange Along the Nucleic Acid Backbone

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Abstract

Phosphorus (31P) NMR spectroscopy can provide important information about the dynamics of nucleic acids. In this communication, we propose an inversely detected 31P transverse relaxation rate (R 2) measurement experiment. This experiment enables fast measurement of accurate 31P transverse relaxation rates and provides the possibility to detect slow motions mapped by the phosphorus nuclei along the nucleic acid backbone. Dispersion curves show some 31P nuclei experiencing chemical exchange in the millisecond time scale. Under the assumption of a two-state exchange process, the reduced lifetimes of the exchanging sites (τex) obtained are in accordance with base pair lifetime estimates deduced from imino proton exchange measurements.

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Author notes

  1. Laurent J. Catoire

    Present address: Laboratoire de Physico-Chimie Moléculaire des Membranes Biologiques, CNRS/Université Paris VII, UMR 7099, Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, 75005, Paris, France

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  1. Laboratoire de Biotechnologie et de Pharmacogénétique Appliquée, CNRS UMR 8113, Institut Gustave Roussy, 39 rue Camille Desmoulins, 94805, Villejuif, France

    Laurent J. Catoire

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Catoire, L.J. Phosphorus-31 Transverse Relaxation Rate Measurements by NMR Spectroscopy: Insight into Conformational Exchange Along the Nucleic Acid Backbone. J Biomol NMR 28, 179–184 (2004). https://doi.org/10.1023/B:JNMR.0000013825.45299.fe

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