Skip to main content
Log in

In vitro assessment of the biological response to nano-sized hydroxyapatite

  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

Nano-sized, rod-like hydroxyapatite (nHA) crystals were produced and shown to be phase-pure by X-ray diffraction analysis, as no secondary phases were observed. The nHA suspension was electrosprayed onto glass substrates using a novel processing routine to maintain nanocrystals of hydroxyapatite. The biocompatibility of nHA was determined using human monocyte-derived macrophages and human osteoblast-like (HOB) cell models. The release of lactate dehydrogenase (LDH) from human monocyte-derived macrophages was measured as an indicator of cytotoxicity. The release of the inflammatory cytokine, tumour necrosis factor alpha (TNF-α) from cells in the presence of nHA crystallites was used as a measure of the inflammatory response. Although there was some evidence of LDH release from human monocyte-derived macrophages when in contact with high concentrations of nHA crystals, there was no significant release of TNF-α. Moreover, nHA-sprayed substrates were able to support the attachment and the growth of HOB cells. These results indicate that nHA crystals may be suitable for intraosseous implantation and offers the potential to formulate enhanced composites for biomedical applications.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. W. Bonfield, Metals Mater. 3 (1987) 712.

    Google Scholar 

  2. W. Bonfield, M. Wang and K. E. Tanner, Acta. Mater. 46 (1998) 2509.

    Google Scholar 

  3. C. Durucan and P. W. Brown, J. Biomed. Mater. Res. 51 (2000) 717.

    Google Scholar 

  4. W. Zhang, S. S. Liao and F. Z. Cui, Chem. Mater. 15 (2003) 3221.

    Google Scholar 

  5. J. Huang, S. N. Jayasinghe, S. M. Best, M. J. Edirisinghe, R. A. Brooks and W. Bonfield, J. Mater. Sci. Lett. 39 (2004) 1029.

    Google Scholar 

  6. J. Sun, Y. Tsuang, W. H. Chang, J. Li, H. Liu and F. Lin, Biomaterials 18 (1997) 683.

    Google Scholar 

  7. J. T. Ninomiya, J. A. Struve, C. T. Stelloh, J. M. Toth and K. E. Crosby, J. Ortho. Res. 19 (2001) 621.

    Google Scholar 

  8. Y. Harada, J. Wang, V. Doppalapudi, A. Willis, M. Jasty, W. Harris, M. Nagase and S. Goldring, J. Biomed. Mater. Res. 32 (1996) 19.

    Google Scholar 

  9. J. A. Wimhurst, R. A. Brooks and N. Rushton, J. Bone Joint Surg. 83-B (2001) 278.

    Google Scholar 

  10. S. N. Jayasinghe AND M. J. Edirisinghe, J. Mater. Res. Innovat. 7 (2003) 62.

    Google Scholar 

  11. S. N. Jayasinghe, M. J. Edirisinghe and T. Dewilde, ibid. 6 (2002) 92.

    Google Scholar 

  12. M. J. Dalby, L. Disilvio, E. J. Harper and W. Bonfield, Biomaterials 23 (2002) 569.

    Google Scholar 

  13. S. N. Jayasinghe and M. J. Edirisinghe, J. Aerosol. Sci. 33 (2002) 1379.

    Google Scholar 

  14. S. N. Jayasinghe and M. J. Edirisinghe, ibid. 35 (2004) 233.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to J. Huang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Huang, J., Best, S.M., Bonfield, W. et al. In vitro assessment of the biological response to nano-sized hydroxyapatite. Journal of Materials Science: Materials in Medicine 15, 441–445 (2004). https://doi.org/10.1023/B:JMSM.0000021117.67205.cf

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/B:JMSM.0000021117.67205.cf

Keywords

Navigation