Abstract
A three-dimensional composite material was produced from microporous coralline origin hydroxyapatite (HA) microgranules, chitosan fibers and chitosan membrane. Cylindrical HA microgranules were oriented along channel direction within multichannel mould space and aligned particles were supported with fibers and a chitosan membrane. The positive replica of mould channels was clasp fixed to produce thicker scaffolds. Light microphotographs of the developed complex structure showed good adhesion between the HA particles, the fibers and the supporting membrane. The composite material showed 88% (w/w) swelling in one hour and preserved the complex structure of the original material upon long-term incubation in physiological medium. MEM extract test of HA chitosan complex showed no cell growth inhibition and cell viability assay (MTS) indicated over 90% cell viability.
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References
D. W. Hutmacher, Biomaterials 21 (2000) 2529.
L. E. Freed, G. Vunjak, R. J. Biron, D. Eagles, D. Lesnoy, S. K. Barlow and R. Langer, Bio Technology 12 (1994) 689.
A. G. Mikos, G. Sarakinis, S. M. Leite, J. P. Vacanti and R. Langer, Biomaterials 14 (1993) 323.
A. G. Mikos and J. S. Temenoff, EJB Electronic J. Biotechnol. 3 (2000) 114.
T. H. Ang, F. S. A. Sultana, D. W. Hutmacher, Y. S. Wong, J. Y. H. Fuh, X. M. Mo, H. T. Loh, E. Burdet and S. H. Teoh, Mater. Sci. Eng. 20 (2002) 35.
M. K. Agarwala, V. R. Jamalabad, N. A. Langrana, A. Safari, P. J. Whalen and S. C. Danforth, Rapid Prototyping J. 4 (1996) 4.
E. Sach, M. Cima, P. Williams, D. Brancazio and J. Cornie, J. Eng. Industry 114 (1992) 481.
L. G. Cima, J. P. Vacanti, C. Vacanti, D. Ingber, D. Mooney, R. Langer, J. Biomed. Eng. 113 (1991) 143.
D. J. Barnes and J. M. Lough, Glob. Change Biol. 6 (1996) 569.
R. Vago, D. Plotquin, A. Bunin, I. Senelnikov, D. Atar and D. Itzhak, J. Biochem. Biophys. Methods 50 (2002) 253.
R. E. Holmes, Plast. Reconstr. Surg. 63 (1979) 626.
J. Vuola, R. Taurino, H. Goransson and S. Askoseljavaara, Biomaterials 19 (1998) 223.
S. Hirano, Polym. Int. 48 (1999) 732.
I. Yamaguchi, K. Tokuchi, H. Fukuzaki, Y. Koyama, K. Takakuda, H. Monhaa and J. Tanaka, J. Biomed. Mater. Res. 55 (2000) 20.
M. Nakajima, H. Yagasaki and A. H. Kafrawy, ibid. 45 (1999) 204.
Y. Zhang and M. Zhang, J. Non-Cryst. Solids 282 (2001) 159.
R. L. Reis, A. M. Cunha and M. J. Bevis, J. Appl. Med. Polym. 2 (1998) 49.
R. L. Reis, A. M. Cunha, P. S. Allan and J. Bevis, Adv. Polym. Technol. 16 (1997) 263.
R. Muzzarrelli, Carbohydr. Polym. 20 (1993) 7.
M. Takanori, K. Takashi and H. Shojiro, JPN Patent 0347263 (1991).
J. Z. Knaul, S. M. Hudson and K. A. M. Creber, J. Appl. Poly. Sci. 72 (1999) 1721.
J. Z. Knaul, M. Hooper, C. Chanyi and A. M. Creber, J. Appl. Polym. Sci. 69 (1998) 1435.
S. Hirano, K. Nagamura, M. Zhang, S. K. Kim, B. G. Chung, M. Yoshikawa and T. Midorikawa, Carbohydr. Polym. 38 (1999) 293.
K. Tuzlakoglu, C. M. Alves, J. F. Mano and R. L. Reis, Macromol. Biosci. (2003) submitted.
ISO/EN 10993-5. Biological evaluation -Part 5. Tests for cytotoxicity: in vitro methods (1992).
A. H. Cory, T. C. Owen, J. A. Barltrop and J. G. Cory, Cancer Commun. 7 (1998) 207.
V. Salih, K. Franks, M. James, G. W. Hastings and J. C. Knowles, J. Mater. Sci: Mater Med. 11 (2000) 615.
A. Lauto, M. Ohebshalom, M. Esposito, J. Mingin, P. S. Li, D. Felsen, M. Goldstein and P. Poppas, Biomaterials 22 (2001) 1869.
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Baran, E.T., Tuzlakoglu, K., Salgado, A.J. et al. Multichannel mould processing of 3D structures from microporous coralline hydroxyapatite granules and chitosan support materials for guided tissue regeneration/engineering. Journal of Materials Science: Materials in Medicine 15, 161–165 (2004). https://doi.org/10.1023/B:JMSM.0000011818.85273.cd
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DOI: https://doi.org/10.1023/B:JMSM.0000011818.85273.cd