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Modification of human platelet adhesion on biomaterial surfaces by protein preadsorption under static and flow conditions

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Abstract

Biomaterial-induced thrombosis remains one of the main complications of vascular implant devices. Preadsorbed proteins on the biomaterial/blood interface will modify the adhesion and activation of platelets (PTLs) during the initial contact-phase. Our results clearly show that PTL-adherence on biomaterials is influenced not only by protein preadsorption, but also by flow conditions. The covalent coating of TCPS and glass by phosphorylcholine (PC) induces a significant decrease of PTL adhesion but leads to a slight, but nevertheless significant activation of PTL, which was detected by the induction of P-selectin expression using FACS analysis. Methodologically, the visualization of PTL adhesion gave more reliable results for measurement of PTL adhesion than the cell-enzyme immunoassay (EIA) for P-selectin. Human citrated plasma caused an inhibition of PTL. It is probable, that the contained sodium citrate may inhibit PTL adhesion by its calcium ion-binding capacity. The flow experiment as dynamic system is in our view absolutely essential for the evaluation of biomaterials for vascular prosthesis, and is in accordance with the international standards. The results of the experiments also suggest that investigations under static and flow conditions are needed to determine the influence of protein adsorption on mixed blood cell populations, for example, on PTL and PMN mixtures/co-cultures in order to achieve a better simulation of the in vivo situation.

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Authors and Affiliations

  1. Institute of Pathology, Johannes Gutenberg-University of Mainz, Langenbeckstr. 1, D-55101, Mainz, Germany and

    Mike Otto, Torsten Hansen & Charles James Kirkpatrick

  2. Department of Molecular Pathology of the WHO Collaborating Centre for Development of Histopathological Classification and Criteria of Rheumatoid Arthritis and Allied Diseases, Institute of Pathology of Trier, Reference Centre for Implant- and Biomaterials-Pathology, Moltkestr. 32, D-54292, TRIER, Germany

    Mike Otto

  3. Clinic of Gynaecology and Obstetrics, Johannes Gutenberg-University of Mainz, Langenbeckstr. 1, D-55101, Mainz, Germany

    Arno Franzen

Authors
  1. Mike Otto
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Correspondence to Mike Otto.

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Otto, M., Franzen, A., Hansen, T. et al. Modification of human platelet adhesion on biomaterial surfaces by protein preadsorption under static and flow conditions. Journal of Materials Science: Materials in Medicine 15, 35–42 (2004). https://doi.org/10.1023/B:JMSM.0000010095.19693.67

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  • DOI: https://doi.org/10.1023/B:JMSM.0000010095.19693.67

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