Abstract
Purpose : To test the effect of cyclic adenosine 3′, 5′-monophosphate (cAMP) on early human ovarian follicles during prolonged culture period.
Methods : Donated ovarian biopsies from 16 women undergoing gynecological laparoscopy were cut into slices and cultured in parallel for 1, 2, or 3 weeks in the presence and the absence of 0.5 mM 8-bromo-cAMP. The developmental stages, sizes, and viability of the follicles were recorded from histological sections of all samples.
Results : On day 14, cortical slices cultured with 8-bromo-cAMP showed a significantly higher proportion of secondary follicles (50.0% vs. 20.0%) and a lower proportion of primordial follicles (9.7% vs. 26.7%) when compared with those cultured without 8-bromo-cAMP. On day 21, the proportion of viable follicles in cortical slices with 8-bromo-cAMP treatment was significantly higher than that without 8-bromo-cAMP treatment (79.6% vs. 55.2%).
Conclusion : CyclicAMP promoted folliculogenesis and follicle survival during 14–21 days' culture of human ovarian cortical slices.
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Zhang, P., Louhio, H., Tuuri, T. et al. In Vitro Effect of Cyclic Adenosine 3′, 5′-Monophosphate (cAMP) on Early Human Ovarian Follicles. J Assist Reprod Genet 21, 301–306 (2004). https://doi.org/10.1023/B:JARG.0000043704.10845.87
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DOI: https://doi.org/10.1023/B:JARG.0000043704.10845.87