Abstract
Purpose : To study if second day intracytoplasmic sperm injection (ICSI) results in chromosomal abnormalities in the embryos.
Methods : Rescue ICSI was performed on 14 metaphase II (MII) oocytes after unsuccessful conventional IVF, four were fertilized. Fluorescent in situ hybridization (FISH) was performed on these four embryos and was informative for three.
Results : There were two tetraploid embryos, one mosaic embryo with trisomy 21, tetrasomy 18, and tetrasomy for sex chromosomes in one cell and trisomy 22 in another cell.
Conclusions : We discourage the use of second day ICSI due to the observed increase in chromosomal abnormalities in these embryos.
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References
Tsirigotis M, Nicholson N, Taranissi M, Bennett V, Pelekanos M, Craft I: Late intracytoplasmic sperm injection in unexpected failed fertilization in vitro: Diagnostic or therapeutic? Fertil Steril 1995;63:816-819
Lundin K, Sjogren A, Hamberger L: Reinsemination of one-day-old oocytes by use of intracytoplasmic sperm injection. Fertil Steril 1996;66:118-121
Morton PC, Yoder CS, Tucker MJ, Wright G, Brockman WD, Kort HI: Reinsemination by intracytoplasmic sperm injection of 1-day-old oocytes after complete conventional fertilization failure. Fertil Steril 1997;68:488-491
Nagy ZP, Joris H, Liu J, Staessen C, Devroey P, Van Steirteghem AC: Intracytoplasmic single sperm injection of 1-day-old unfertilized human oocytes. Hum Reprod 1993;8:2180-2184
Yuzpe AA, Liu Z, Fluker MR: Rescue intracytoplasmic sperm injection (ICSI)-salvaging in vitro fertilization (IVF) cycles after total or near-total fertilization failure. Fertil Steril 2000;73:1115-1119
Nagy ZP, Staessen C, Liu J, Joris H, Devroey P, Van Steirteghem AC: Prospective, auto-controlled study on reinsemination of failed-fertilized oocytes by intracytoplasmic sperm injection. Fertil Steril 1995;64:1130-1135
Pellicer A, Simon C, Miro F, Castellvi RM, Ruiz A, Ruiz M, Perez M, Bonilla-Musoles F: Ovarian response and outcome of in-vitro fertilization in patients treated with gonadotrophin-releasing hormone analogues in different phases of the menstrual cycle. Hum Reprod 1989;4:285-289
Vidal F, Gimenez C, Rubio C, Simon C, Pellicer A, Santalo J, Egozcue J: FISH preimplantation diagnosis of chromosome aneuploidy in recurrent pregnancy wastage. J Assist Reprod Genet 1998;15:310-313
Rawe VY, Olmedo SB, Nodar FN, Doncel GD, Acosta AA, Vitullo AD: Cytoskeletal organization defects and abortive activation in human oocytes after IVF and ICSI failure. Mol Hum Reprod 2000;6:510-516
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Pehlivan, T., Rubio, C., Ruiz, A. et al. Embryonic Chromosomal Abnormalities Obtained After Rescue Intracytoplasmic Sperm Injection of 1-Day-Old Unfertilized Oocytes. J Assist Reprod Genet 21, 55–57 (2004). https://doi.org/10.1023/B:JARG.0000025939.26834.93
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DOI: https://doi.org/10.1023/B:JARG.0000025939.26834.93