Abstract
Purpose : To determine the viability of long-term, stored serum, which is used in human in vitro production as a source of energy substrates, amino acids, vitamins, growth factors, and other nutrients.
Method : Two-cell mouse embryos were used in this prospective, cohort study. Serum stored for 1-, 5-, 10-, and 12-year intervals was subjected to four replications of mouse-embryo testing.
Result(s) : There were no significant differences in blastocyst rates between any of the replicates by year (80–100% blastocyst rate; P > 00.1) or between pooled replicate means by time period.
Conclusion(s) : Serum may be frozen up to 12 years.
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Graves, J.E., Higdon, H.L., Johnson, J.E. et al. Longevity of Donor Serum. J Assist Reprod Genet 21, 51–53 (2004). https://doi.org/10.1023/B:JARG.0000025938.67218.47
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DOI: https://doi.org/10.1023/B:JARG.0000025938.67218.47