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Stress resistance induced by paramylon treatment in Artemia sp.

Abstract

A study was undertaken to investigate the effectiveness of paramylon extracted from the non-photosynthetic WZSL mutant of Euglena gracilis in potentiating the resistance of the brine shrimp Artemia sp. to stress conditions resulting from poor growth medium quality and daily handling. The experimental design incorporated four different treatments: I) paramylon addition/no growth medium replacement; II) no paramylon addition/no growth medium replacement; III) paramylon addition/growth medium replacement; IV) noparamylon addition/growth medium replacement. As shown by the survival curves of Artemia sp., the addition of paramylon significantly reduced susceptibility of animals to the stressors. Paramylon effectively increased also the ability of offspring to withstand poor water quality and handling damages. Without paramylon almost all offspring died before adulthood, whereas addition of paramylon allowed the animals to moult to the adult stage. Moreover, reproductive success (higher number of living offspring) was enhanced considerably in animals treated with paramylon treated under both stress conditions. These results show that paramylon extracted from Euglena represents a promising biologically active compound for aquaculture purposes. It could be used as a purified product or as component of whole cells, since the Euglena mutant, because of the high intracellular amount of paramylon it can accumulate, could be added to the feed or to water in tanks and ponds without prior processing.

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Correspondence to Paolo Gualtieri.

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Vismara, R., Vestri, S., Frassanito, A.M. et al. Stress resistance induced by paramylon treatment in Artemia sp.. Journal of Applied Phycology 16, 61–67 (2004). https://doi.org/10.1023/B:JAPH.0000019113.77013.0d

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  • Artemia
  • β-1,3-glucan
  • Euglena gracilis
  • immunostimulant
  • paramylon
  • stress
  • WZSL mutant