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Changes in cytokinin and auxin concentrations in seaweed concentrates when stored at an elevated temperature

Journal of Applied Phycology volume 16, pages 31–39 (2004)Cite this article

Abstract

Two seaweed concentrates were made from the kelps Ecklonia maxima and Macrocystis pyrifera using a cell burst method. Cytokinin- and auxin-like activities were measured using the soybean callus and mungbean bioassays, respectively. Cytokinin-like activity was detected in both seaweed concentrates, being equivalent to approximately 50 μg L−1 kinetin. Auxin-like activity was also detected in both concentrates with the E. maxima derived concentrate having higher biological activity, equivalent to 10−5–10−4 M indole-butyric acid. Two replicates of each concentrate were stored at 54 °C for 14 days to accelerate the effects of storage. Both fresh and stored samples of the two seaweed concentrates were analysed for their endogenous cytokinin and auxin content. The samples were purified using a combined DEAE-Sephadex octadecylsilica column and immunoaffinity chromatography based on wide-range cytokinin and IAA specific monoclonal antibodies. These extracts were analysed by HPLC linked to a Micromass single quadrupole mass spectrophotometer. Eighteen and nineteen different cytokinins were detected, respectively, in the two concentrates, with trans-zeatin-O-glucoside being the main cytokinin present. Accelerated storage of the concentrates caused an increase in the total cytokinin concentration with a large increase in the aromatic meta-topolin. Indole-3-acetic acid was the main auxin in both seaweed concentrates. Indole conjugates, including amino acid conjugates, were also quantified. The total auxin concentration decreased with accelerated storage for both concentrates.

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References

  • Abe H, Uchiyama M, Sato R (1972) Isolation and identification of native auxins in marine algae. Agric. biol. Chem. 36: 2259-2260.

    CAS  Google Scholar 

  • Auer CA (1997) Cytokinin conjugation: recent advances and patterns in plant evolution. Plant Growth Reg. 23: 17-32.

    Article  CAS  Google Scholar 

  • Bartel B (1997) Auxin biosynthesis. Ann Rev. Plant Physiol. Plant Mol. Biol. 48: 51-66.

    Article  CAS  Google Scholar 

  • Crouch IJ, Smith MT, van Staden J, Lewis MJ, Hoad GV (1992) Identification of auxins in a commercial seaweed concentrate. J. Plant Physiol. 139: 590-594.

    CAS  Google Scholar 

  • Crouch IJ, van Staden J (1993) Evidence for the presence of plant growth regulators in commercial seaweed products. Plant Growth Reg. 13: 21-29.

    CAS  Google Scholar 

  • Crouch IJ, van Staden J (1994) Commercial seaweed products as biostimulants in horticulture. J. Home Consumer Hort. 1: 19-75.

    Google Scholar 

  • Duan D, Lui X, Pan F, Lui H, Chen N, Fei X (1995) Extraction and identification of cytokinin from Laminaria japonicaAresch. Bot. mar. 38: 409-412.

    Article  CAS  Google Scholar 

  • Faiss M, Zalubilová J, Strnad M, Schmulling T (1997) Conditional expression of the iptgene indicates a function for cytokinins in paracrine signalling in whole tobacco plants. Plant J. 12: 401-415.

    Article  PubMed  CAS  Google Scholar 

  • Featonby-Smith FC, van Staden J (1984) Identification and seasonal variation of endogenous cytokinins in Ecklonia maxima(Osbeck) Papenf. Bot. mar. 27: 527-531.

    Article  CAS  Google Scholar 

  • Florence AT, Attwood D (1988) Physicochemical Principles of Pharmacy. 2nd edition. MacMillan Press, UK, pp. 81-129.

    Google Scholar 

  • Hess CE (1964) The mung bean bioassay for the detection of root promontory substances. Plant Physiol. (Suppl.) 36: XXI.

    Google Scholar 

  • Holub J, Hanuš J, Hanke DE, Strnad M (1998) Biological activity of cytokinins derived from ortho-and meta-hydroxybenzyladenine. Plant Growth Reg. 26: 109-115.

    Article  CAS  Google Scholar 

  • Jacobs WP (1993) A search for some angiosperm hormones and their metabolites in Caulerpa paspaloides(Chlorophyta). J. Phycol. 29: 595-600.

    Article  CAS  Google Scholar 

  • Jacobs WP, Falkenstein K, Hamilton RH (1985) Nature and amount of auxin in algae. Plant Physiol. 78: 844-848.

    Article  PubMed  CAS  Google Scholar 

  • MacDonald EMS, Akiyoishi DE Morris RO (1981) Combined high-performance liquid chromatography-radioimmunoassay for cytokinins. J. Chromatogr. 214: 101-109.

    Article  CAS  Google Scholar 

  • Máthé A (1995) Storage of medicinal and aromatic plants: an important item of good agricultural and good manufacturing practice. Storage ofMedicinal PlantsWorkshop, Halle/Saale. pp. 5-18.

  • Mazur H, Konop A, Synak R (2001) Indole-3-acetic acid in the culture medium of two axenic green microalgae. J. appl. Phycol. 13: 35-42.

    Article  CAS  Google Scholar 

  • Metting B, Zimmerman WJ, Crouch IJ, van Staden J (1990) Agronomic uses of seaweed and microalgae. In: Akatsuka I (ed.). Introduction to Applied Phycology. SPB Academic Publishing, The Hague, The Netherlands, pp. 589-627.

    Google Scholar 

  • Miller CO (1965) Evidence for the natural occurrence of zeatin and derivatives: Compounds from maize which promote cell division. Proc. natl Acad. Sci. USA 54: 1052-1058.

    Article  PubMed  CAS  Google Scholar 

  • Normanly J (1997) Auxin metabolism. Physiol. Plant. 100: 431-442.

    Article  CAS  Google Scholar 

  • Novák O, Tarkowski P, Tarkowská D, Dolezal K, Lenobel R, Strnad M (2003) Quantitative analysis of cytokinins in plants by liquid chromatography/single-quadrupole mass spectrometry. Anal. Chim. Acta 480: 207-218.

    Article  CAS  Google Scholar 

  • Sanderson KJ, Jameson PE (1986) The cytokinins in a liquid seaweed extract: Could they be the active ingredients? Acta Hort. 179: 113-116.

    Google Scholar 

  • Sanderson KJ, Jameson PE, Zabkiewicz JA (1987) Auxins in a seaweed extract: Identification and quantification of indole-3-acetic acid by gas chromatography-mass spectrometry. J. Plant Physiol. 129: 363-367.

    CAS  Google Scholar 

  • Stirk WA, van Staden J (1997a) Comparison of cytokinin-and auxin-like activity in some commercially used seaweed extracts. J. appl. Phycol. 8: 503-508.

    Article  Google Scholar 

  • Stirk WA, van Staden J (1997b) Isolation and identification of cytokinins in a new commercial seaweed product made from Fucus serratusL. J. appl. Phycol. 9: 327-330.

    Article  CAS  Google Scholar 

  • Stirk WA, van Staden J (2003) Cytokinins in macroalgae. Plant Growth Reg.: in press.

  • Strnad M (1997) The aromatic cytokinins. Physiol. Plant. 101: 674-688.

    Article  CAS  Google Scholar 

  • Tay SAB, MacLeod JK, Palni LMS, Letham DS (1985) Detection of cytokinins in a seaweed extract. Phytochem. 24: 2611-2614.

    Article  CAS  Google Scholar 

  • Tay SAB, Palni LMS, MacLeod JK (1987) Identification of cytokinin glucosides in a seaweed extract. J. Plant Growth Reg. 5: 133-138.

    Article  CAS  Google Scholar 

  • van Staden J, Drewes FE (1991) The biological activity of cytokinin derivatives in the soybean callus bioassay. Plant Growth Reg. 10: 109-115.

    Article  CAS  Google Scholar 

  • Zhang W, Chapman DJ, Phinney BR, Spray CR (1991) Identification of cytokinins in Sargassum muticum(Phaeophyta) and Porphyra perforata(Rhodophyta). J. Phycol. 27: 87-91.

    Article  CAS  Google Scholar 

  • Zhang W, Yamane H, Chapman DJ (1993) The phytohormone profile of the red alga Porphyra perforata. Bot. mar. 36: 257-266.

    Article  CAS  Google Scholar 

  • Zhang W, Yamane H, Takahashi N, Chapman DJ, Phinney BO (1989) Identification of a cytokinin in the green alga Chara globularis. Phytochem. 28: 337-338.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Research Centre for Plant Growth and Development, University of Natal Pietermaritzburg, P/Bag X01, Scottsville, 3209, South Africa

    W. A. Stirk, G. D. Arthur & J. van Staden

  2. Kelp Products (Pty) Ltd., P.O. Box 325, Simon's Town, 7995, South Africa

    A. F. Lourens

  3. Laboratory of Growth Regulators, Palacky University and Institute of Experimental Botany ASCR, Slechtitelu 11, 783 71, Olomouc, Czech Republic

    O. Novák & M. Strnad

Authors
  1. W. A. Stirk
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  2. G. D. Arthur
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  3. A. F. Lourens
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  4. O. Novák
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  5. M. Strnad
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  6. J. van Staden
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Correspondence to W. A. Stirk.

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Stirk, W.A., Arthur, G.D., Lourens, A.F. et al. Changes in cytokinin and auxin concentrations in seaweed concentrates when stored at an elevated temperature. Journal of Applied Phycology 16, 31–39 (2004). https://doi.org/10.1023/B:JAPH.0000019057.45363.f5

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  • DOI: https://doi.org/10.1023/B:JAPH.0000019057.45363.f5

  • accelerated storage
  • auxins
  • cytokinins
  • Ecklonia maxima
  • Macrocystis pyrifera
  • seaweed concentrate