Abstract
Samples from a total of 134 lakes, practically all freshwater lakes in Åland, SW Finland, were screened for microcystins, hepatotoxic peptides of cyanobacteria, using high performance liquid chromatography (HPLC), time-resolved fluoroimmunoassay (TR-FIA), and protein phosphatase inhibition (PPIA) during two summers. Microcystins were recorded in 4 of 54 lakes in 1999 and 14 of 134 lakes in 2000, especially in eutrophic lakes but also in some dystrophic lakes and one oligotrophic. There was a satisfactory agreement between results obtained with different methods. The most likely microcystin-producers involved, as judged by microscopical examination, were Planktothrix agardhii, Microcystis aeruginosa, and Anabaena lemmermannii. One drinking-water reservoir and several lakes used for irrigation were microcystin-positive. Microcystins were also detected in late autumn samples from some eutrophic lakes.
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References
Carmichael, W.W., 1996. Toxic Microcystis and the environment. In Watanabe, M. F. (ed.), Toxic Microcystis. CRC Press, Inc. Boca Raton: 1–11.
Cedercreutz, C., 1934. Die Algenflora und Algenvegetation auf Åland. Acta Bot. Fenn. 15: 1–120.
Cedercreutz, C., 1947. Die Gefässpflanzenvegetation der Seen auf Åland. Acta Bot. Fenn. 38: 1–77.
Chorus, I., 2001. Cyanotoxins – occurrence, causes, consequences. Springer, Berlin. 357 p.
Chorus, I. & J. Bartram, 1999. Toxic cyanobacteria in water. A guide to their public health consequences, monitoring, and management. WHO. E & FN Spon, London. 416 p.
Codd, G. A., S. G. Bell, K. Kaya, C. J. Ward, K. A. Beattie & J. S. Metcalf, 1999. Cyanobacterial toxins, exposure routes and human health. Eur. J. Phycol. 34: 405–415.
Eriksson, J. E., J. A. O. Meriluoto & T. Lindholm, 1986. Can cyanobacterial toxins accumulate in aquatic food chains? In Megusar, F. & M. Gantar (eds), Perspectives in Microbial Ecology. Proc. 4th Int. Symp. Microbial Ecol., Ljubljana, Yugoslavia: 655–658.
Fontal, O. I., M. R. Vieytes, J. M. V. Baptista de Sousa, M. C. Louzao & L. M. Botana, 1999. A fluorescent microplate assay for microcystin-LR. Anal. Biochem. 269: 289–296.
Gee, K. R., W.-C. Sun, M. K. Bhalgat, R. H. Upson, D. H. Klaubert, K. A. Latham & R. P. Haugland, 1999. Fluorogenic substrates based on fluorinated umbelliferones for continuous assays of phosphatases and ?-galactosidases. Anal. Biochem. 273: 41–48.
Kotak, B., R. W. Zurawelli, E. E. Prepas & C. F. B. Holmes, 1996. Microcystin-LR concentration in aquatic food web compartments from lakes of varying trophic status. Can. J. Fish. aquat. Sci. 53: 1974–1985.
Kukkonen, J., 1999. Syanobakteerien maksatoksiinien osoitusmenetelmien vertailu (in Finnish). Finnish Environment Institute, Helsinki. 64 p.
Lampert, W., 1981. Inhibitory and toxic effects of blue-green algae on Daphnia. Int. Rev. ges. Hydrobiol. 66: 285–298.
Lindholm, T., 1991. Från havsvik till insjö. Miljöförlaget, Helsinki/ Helsingfors. 160 p.
Lindholm, T., 1996. A survey of small lakes in the archipelago of NW Åland (SW Finland). Memoranda Soc. Fauna Flora Fenn. 72: 31–36.
Lindholm, T., M. Degerlund, L. Spoof & J. Meriluoto, 2002. A century of water quality changes in a coastal lake with toxic Planktothrix. Verh. int. Ver. Limnol., 28: 984–987.
Lindholm, T. & J. E. Eriksson, 1990. Limnological changes in two recently isolated lakes. Limnologica 20: 273–278.
Lindholm, T., J. E. Eriksson & J. A. O. Meriluoto, 1989. Toxic cyanobacteria and water quality problems – examples from a eutrophic lake on Åland, South West Finland. Water Res. 23: 481–486.
Lindholm, T. & J. A. O. Meriluoto, 1991. Recurrent depth maxima of the hepatotoxic cyanobacterium Oscillatoria agardhii. Can. J. Fish. aquat. Sci. 48: 1629–1634.
Lindholm, T., P. Öhman, K. Kurki-Helasmo, B. Kincaid & J. Meriluoto, 1999. Toxic algae and fish mortality in a brackishwater lake in Åland, SW Finland. Hydrobiologia 397: 109–120.
Mehto, P., M. Ankelo, A. Hinkkanen, A. Mikhailov, J. E. Eriksson, L. Spoof & J. Meriluoto, 2001. A time-resolved fluoroimmunometric assay for detection of microcystins, cyanobacterial peptide hepatotoxins. Toxicon 39: 831–836.
Meriluoto, J., 2000. Toxins of freshwater cyanobacteria (bluegreen algae). In Bogusz, M. J. (ed.), Handbook of Analytical Separations, vol. 2: Forensic Science. Elsevier, Amsterdam: 359–390.
Meriluoto, J., L. Lawton & K.-i. Harada, 2000. Isolation and detection of microcystins and nodularins, cyanobacterial peptide hepatotoxins. In Holst, O. (ed.), Bacterial Toxins: Methods and Protocols. Humana Press, Totowa, NJ: 65–87.
Mikhailov, A., A.-S. Härmälä-Braskén, J. Meriluoto, Y. Sorokina, D. Dietrich & J. E. Eriksson, 2001. Production and specificity of mono and polyclonal antibodies against microcystins conjugated through N-methyldehydroalanine. Toxicon 39: 477–483.
Pflugmacher, S., C. Wiegand, K. A. Beattie, G. A. Codd & C. E. W. Steinberg, 1998. Uptake of cyanobacterial hepatotoxin microcystin-LR by aquatic macrophytes. J. Appl. Bot. 72: 228–232.
Sipiä, V., H. Kankaanpää, K. Lahti, W. W. Carmichael & J. Meriluoto, 2001. Detection of nodularin in flounders and cod from the Baltic Sea. Environ. Toxicol. 16: 121–126.
Sivonen, K., S. I. Niemelä, R. M. Niemi, L. Lepistö, T. H. Luoma & L. A. Räsänen, 1990. Toxic cyanobacteria (blue-green algae) in Finnish fresh and coastal waters. Hydrobiologia 190: 267–275.
Spoof, L., K. Karlsson & J. Meriluoto, 2001. High-performance liquid chromatographic separation of microcystins and nodularin, cyanobacterial peptide toxins, on C18 and amide C16 sorbents. J. Chromatogr. A 909: 225–236.
Tikkanen, T. & T. Willén, 1992. Växtplanktonflora. Statens Naturvårdsverk, Solna, Sweden. 280 p.
Ueno, Y., S. Nagata, T. Tsutsumi, A. Hasegawa, F. Yoshida, M. Suttajt, D. Mebs, M. Pütsch & V. Vasconcelos, 1996 a. Survey of microcystins in environmental water by a highly sensitive immunoassay based on monoclonal antibody. Natural Toxins 4: 271–276.
Ueno, Y., S. Nagata, T. Tsutsumi, A. Hasegawa, M. F. Watanabe, H-D. Park, G-C. Chen & S-Z. Yu, 1996 b. Detection of microcystins, a blue-green algal hepatotoxin, in drinking water sampled in Haimen and Fusui, endemic areas of primary liver cancer in China, by highly sensitive immunoassay. Carcinogenesis 47: 1317–1321.
Willén, T. & R. Mattsson, 1997. Water-blooming and toxinproducing cyanobacteria in Swedish fresh and brackish waters 1981–1995. Hydrobiologia 353: 181–192.
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Lindholm, T., Vesterkvist, P., Spoof, L. et al. Microcystin occurrence in lakes in Åland, SW Finland. Hydrobiologia 505, 129–138 (2003). https://doi.org/10.1023/B:HYDR.0000007301.89200.ad
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DOI: https://doi.org/10.1023/B:HYDR.0000007301.89200.ad