Abstract
The growth and flowering response of a cold-requiring cauliflower (Brassica oleracea var. botrytis cv. ‘60 day’) to a range of temperatures under 10 h photoperiod and to growth regulator application were investigated. Endogenous gibberellin A1(GA1) concentrations were also assessed under these treatments. Flowering and growth of the inflorescence stalk were correlated with plant developmental stage at the time of a vernalizing cold treatment. Temperature and its duration also affected flowering and inflorescence development. The most effective temperature for inflorescence induction was 10 °C. Flowering did not occur in non-vernalized plants (25 °C) even though they had been treated with GA3. Application of GA3 promoted inflorescence stalk elongation greatly in vernalized plants (10 °C), but less so in partially vernalized plants (15 °C or 20 °C). Paclobutrazol (PP333) sprayed at the 8–9 leaf stage significantly suppressed inflorescence stalk length and slightly delayed flower bud formation and anthesis. Vernalization at 10 °C increased endogenous GA1 content in both leaves and the inflorescence stalk irrespective of GA3 or PP333 treatment. Application of GA3 tended to increase GA1 levels, while PP333 significantly reduce GA1, both irrespective of vernalization. Vernalization is an important factor for flowering, but not curd formation in this cauliflower cv. ‘60 day’ and GA1 is likely a causal factor in inflorescence stalk elongation.
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References
Atherton J.G., Hand D.J. and Williams C.A. 1987. Curd initiation in the cauliflower (Brassica oleracea var. botrytis L.). In: Atherton J.G. (ed.), Manipulation of Flowering. Butterworths, London, pp. 133–145.
Chandler J. and Dean C. 1994. Factors influencing the vernalization response and flowering time of late flowering mutants of Arabidopsis thaliana (L.) Heynh. J. Exp. Bot. 45: 1279–1288.
Cheng K.H. and More E.L. 1967. Relation of seedling size and length of cold exposure to the incidence of flowering in Brassica oleracea L. var. acephala DC. Proc. Am. Soc. Hort. Sci. 91: 363–367.
Dahanayake S.R. and Galwey N.W. 1999. Effects of interactions between low-temperature treatment, gibberellin (GA3) and photoperiod on flowering and stem height of spring rape (Brassica napus var. annua). Ann. Bot. 84: 321–327.
Fernandez J.A., Banon S., France J.A., Gonzalez A. and Martinez P.F. 1997. Effects of vernalization and exogenous gibberellins on curd induction and carbohydrate levels in the apex of cauliflower (Brassica oleracea var. botrytis). Sci. Hort. 70: 223–230.
Friend D.J.C. 1985. Brassica. In: Halevy A.H. (ed.), CRC Handbook of Flowering, Vol. 2. CRC Press, Boca Raton, FL, pp. 48–62.
Gaskin P. and MacMillan J. 1991. GC-MS of the Gibberellina and Related Compounds: Methodology and a Library of Spectra. Cantock's Enterprises, Bristol, UK.
Grindal G., Ernstsen A., Reid J.B., Junttila O., Lindgard B. and Moe R. 1998. Endogenous gibberellin A1 levels control thermoperiodic stem elongation in Pisum sativum. Physiol. Plant. 102: 523–531.
Hamano M., Yamato Y., Yamazaki H. and Miura H. 2002. Endogenous gibberellins and their effects on flowering and stem elongation in cabbage (Brassica oleracea var. capitata). J. Hort. Sci. Biotechnol. 77: 220–225.
Hand D.J. and Atherton J.G. 1987. Curd initiation in the cauliflower. I. Juvenility. J. Exp. Bot. 38: 2050–2058.
Hedden P. and Kamiya Y. 1997. Gibberellin biosynthesis: enzymes, genes and their regulation. Ann. Rev. Plant Physiol. Plant Mol. Biol. 48: 431–460.
Kato T. 1964. On the flower head formation and development of cauliflower plants. I. Ecological studies on the flower head formation and development. J. Jpn. Soc. Hort. Sci. 33: 316–326 (in Japanese with English summary).
King R.W. and Evans L.T. 2003. Giobberellins and flowering of grasses and cereals: prizing open the lid of the ''florigen'' black box. Ann. Rev. Plant Biol. 54: 307–328.
Lang A. 1965. Physiology of flower initiation. In: Ruhland W. (ed.), Encyclopedia of Plant Physiology (XV/1). Springer, Berlin, pp. 1380–1536.
Mander L.N., Rood S.B. and Pharis R.P. 1991. Bolting and floral induction in annual and cold-requiring biennial Brassica spp.: effect of photoperiod and exogenous gibberellin. In: Karssen C.M., van Loon L.C. and Vreugdenhil D. (eds), Progress in Plant Growth Regulation. Kluwer Acadmic Publishers, Amsterdam, pp. 371–379.
Nishijima T., Katsura N., Koshioka M., Yamazaki H. and Mander L.N. 1997. Effects of uniconazole and GA3 in coldinduced stem elongation and flowering of Raphanus sativus L. Plant Growth Regul. 21: 207–214.
Rood S.B., Mandel R. and Pharis R.P. 1989. Endogenous gibberellins and shoot growth and development in Brassica napus. Plant Physiol. 89: 269–273.
Ross J.J., Reid J.B., Gaskin P. and MacMillan J. 1989. Internode length in Pisum. Estimation of GA1 levels in genotypes Le, le and led. Physiol. Plant. 76: 173–176.
Sadik S. 1961. Morphology of the curd of cauliflower. Am. J. Bot. 40: 290–297.
Sadik S. 1967. Factors involved in curd and flower formation in cauliflower. Proc. Am. Soc. Hort. Sci. 90: 252–259.
Shah G.A., Guo D.P. and Zeng G.W. 1993a. Curd formation and flowering of plantlets regenerated from cauliflower curd explants. HortScience 28: 677.
Shah G.A., Zeng G.W., Li S.J. and Guo D.P. 1993b. Effects of GA3 and PP333 on inflorescence growth and pod formation of cauliflower. J. Zhejiang Agric. Univ. 19: 351–358(in Chinese with English abstract).
Suge H. 1980. Inhibition of flowering and growth in pharbitis nil by the growth retardant. Ancymidol. Plant Cell Physiol. 21: 1187–1192.
Suge H. and Rappaport L. 1968. Role of gibberellins in stem elongation and flowering in radish. Plant Physiol. 43: 1208–1214.
Talon M. and Zeevaart J.A.D. 1990. Gibberellins and stem growth as related to photoperiod in Silene armeria L. Plant Physiol. 92: 1094–1100.
Wiebe H.J. 1972. Effect of temperature and light on growth and development of cauliflower. II. Optimal vernalization temperature and exposure time. Gartenbauwissenschaft 37: 293–303.
Wilson R.N., Heckman J.W. and Somerville C.R. 1992. Gibberellin is required for flowering in Arabbidopsis thaliana under short days. Plant Physiol. 100: 403–408.
Wurr D.C.E., Fellows J.R. and Crisp P. 1982. Leaf and curd production in cauliflower varieties cold-treated before transplanting. J. Agric. Sci. 99: 425–432.
Zeevaart J.A.D. 1983. Gibberellins and flowering. In: Crozier, A (ed.), The Biochemistry and Physiology of Gibberellins, Vol. 2. Praeger Publications, East Sussex, pp. 333–374.
Zeevaart J.A.D., Gage D.A. and Talon M. 1993. Gibberellin A1 is required for stem elongation in spinach. Proc. Natl. Acad. Sci. USA 90: 7401–7405.
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Guo, DP., Ali Shah, G., Zeng, GW. et al. The Interaction of Plant Growth Regulators and Vernalization on the Growth and Flowering of Cauliflower (Brassica oleracea var. botrytis). Plant Growth Regulation 43, 163–171 (2004). https://doi.org/10.1023/B:GROW.0000040114.84122.11
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DOI: https://doi.org/10.1023/B:GROW.0000040114.84122.11