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Gibberellin signal in barley aleurone: early activation of PLC by G protein mediates amylase secretion

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Abstract

The early events related to intracellular GA-signals in aleurone are not clearly established. We demonstrate that GA treatment induced increases in phosphatidylinositol 4P, 5-kinase (PtdInsP-k), diacylglycerol kinase (DAG-k) and phosphatidate kinase (PA-k) activities in barley aleurone within 5 min. The response to GA was also observed as a rapid and transient InsPs/InsP 3 time-dependent accumulation. U73122, a phospholipase C (PLC) inhibitor, reduced the InsPs and InsP 3 levels and amylase secretion. The G protein activator Mas7 was able to trigger the α-amylase secretion as strongly as GA did; U73122, also reduced this effect. ABA evoked only an increase in phosphatidic acid (PtdOH) and diacylglycerol pyrophosphate (DGPP) levels. This is the first time that a rapid and transient response to GA in correlation with amylase secretion, involving PLC and G protein as well as PA-k activity in the GA signalling pathway, has been demonstrated in aleurone cells.

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Authors and Affiliations

  1. Química Biológica, Argentina

    Ana L. Villasuso, María Lía Molas & Graciela Racagni

  2. 5800 Río Cuarto, Córdoba, Argentina

    Guillermina Abdala

  3. 358 467-6232))

    Estela Machado-Domenech

Authors
  1. Ana L. Villasuso
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  2. María Lía Molas
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  3. Graciela Racagni
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  4. Guillermina Abdala
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  5. Estela Machado-Domenech
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Villasuso, A.L., Lía Molas, M., Racagni, G. et al. Gibberellin signal in barley aleurone: early activation of PLC by G protein mediates amylase secretion. Plant Growth Regulation 41, 197–205 (2003). https://doi.org/10.1023/B:GROW.0000007505.37356.89

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